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GB/T 17999.9-2008 English PDF

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Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 17999.9-2008 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.

US$139.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 17999.9-2008: SPF chicken -- Microbiological surveillance -- Part 9: Tube agglutination test for SPF chicken
Status: Valid

GB/T 17999.9: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 17999.9-2008	139	Add to Cart	3 days	SPF chicken -- Microbiological surveillance -- Part 9: Tube agglutination test for SPF chicken	Valid
GB/T 17999.9-1999	199	Add to Cart	2 days	SPF chicken--Indirect immunofluorescent assay	Obsolete

Similar standards

GB/T 25165 NY/T 1663 GB/T 20365 GB/T 17999.2 GB/T 17999.3 GB/T 17999.1

Basic data

Standard ID: GB/T 17999.9-2008 (GB/T17999.9-2008)
Description (Translated English): SPF chicken -- Microbiological surveillance -- Part 9: Tube agglutination test for SPF chicken
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 6,696
Date of Issue: 2008-12-31
Date of Implementation: 2009-05-01
Older Standard (superseded by this standard): GB/T 17999.8-1999
Regulation (derived from): National Standard Approval Announcement 2008 No.27 (Total No.140)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the technical requirements for tube agglutination test. This section applies to the SPF chickens Salmonella pullorum serum antibodies. Serum plate agglutination test for Salmonella pullorum antibody test conducted, the results difficult to judge, can be carried out in this part of the re-examination.

GB/T 17999.9-2008: SPF chicken -- Microbiological surveillance -- Part 9: Tube agglutination test for SPF chicken

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
. SPF chicken Microbiological surveillance Part 9. Tube agglutination test for SPF chicken ICS 11.220 B41 National Standards of People's Republic of China Replacing GB/T 17999.8-1999 SPF chickens microbiological monitoring Part 9. SPF chickens tube agglutination test Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 17999 "SPF chickens microbiological monitoring" is divided into 10 parts. --- The first part 1. SPF chicken microbiological monitoring General; Part --- Article 2. SPF chicken hemagglutination inhibition test; Part --- Section 3. SPF chicken serum neutralization test; Part --- Chapter 4. SPF chicken serum plate agglutination test; Part --- Article 5. SPF chicken agar diffusion test; --- Part 6. SPF chickens ELISA; Part --- Article 7. SPF chicken embryo susceptibility testing; Part --- Article 8. SPF chicken Salmonella testing; --- Part 9. SPF chickens tube agglutination test; Part --- Article 10. SPF chickens indirect immunofluorescence assay. This is Part 9 GB/T 17999 of. This Part replaces GB/T 17999.8-1999 "SPF chickens tube agglutination test." This section compared with the GB/T 17999.8-1999 The main changes are as follows. --- Added Appendix A "Preparation of Reagents"; --- Modify the judgment agglutination test results; --- Remove chicken Salmonella typhimurium test content; --- In scope to further clarify the situation in this part. This section of Appendix A normative appendix. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Animal Epidemic Prevention Standardization Technical Committee (SAC/TC181) centralized. This section is drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China Animal Health and Epidemiology Center, Jinan Spa law Adams Poultry Limited. The main drafters of this section. song even East, Jiang Qian, Han Lingxia, satellite Shao, Zhu fruit, single Zhong Fang, Liu Jiasen Secretary Chand, Guodong Chun, Yu Haibo, Mengqing Wen. This part of the standard replaces the previous editions are. --- GB/T 17999.8-1999. SPF chickens microbiological monitoring Part 9. SPF chickens tube agglutination test

1 Scope

This section GB/T 17999 specifies the technical requirements of the tube agglutination test. This section applies to SPF chickens Salmonella serum antibodies. Serum plate agglutination test carried out to detect Salmonella antibody, the result is difficult to judge, it can be part of this re-examination. Principle 2 After the bacterial antigen and the corresponding antibody binding, an appropriate amount of electrolyte in the participation, after a period of time appear visible agglutination, The results determined in agglutination titer expressed.

3 Reagents and Equipment

3.1 Reagents Pullorum antigen (bacteria 109CFU), the standard positive and negative sera, sera were seized, 0.5% phenol saline (see preparation method Appendix A). 3.2 Equipment Tube (diameter 8mm ~ 10mm). Procedure 4 4.1 take five test tubes, the first one carbolic acid saline 1.8mL, plus the rest of the tube 1mL, plus the first one was seized serum 0.2mL, and Carbolic acid saline mix (1.10 dilution), moved to take 1mL first two consecutive dilution through 4, from 4 aspirate 1mL Discarded, the tubes last serum dilution were 1.20,1.40,1.80,1.160,1.320. 4.2 antigen solution was added to each tube 1mL. 4.3 Antigen Control. 1mL plus antigen solution in a test tube, add 0.5% phenol saline 1mL. 4.4 positive serum control. A positive serum was diluted to a working concentration (according to instructions). Plus antigen solution in a test tube 1mL, diluted Yang Serum 1mL. 4.5 Negative serum control. with positive serum dilution use. 4.6 After shaking the test tube at 37 ℃ incubator incubation, 24h later moved to 4 ℃ ~ 8 ℃ refrigerator overnight. 4.7 Results observation. antigen control results and negative serum controls, showed "-", the positive control serum showed varying degrees of aggregation, show trials established. The results are shown in Table 1 agglutination judgment. Agglutination titer to render "++" serum agglutination test tube as the highest dilution of the serum agglutination titer. Table 1 agglutination test result determination Category result of the determination in vitro findings 1 big block of aggregation, the supernatant liquid is completely transparent ++++ 2 significant aggregates, the supernatant was 75% transparency +++ 3 show no visible agglutination particles, supernatant transparency up to 50% + Positive 4 slight visible aggregate particles, the supernatant was suspicious transparency of 25% ± 5 No aggregates, uniform turbidity liquid - negative ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 17999.9-2008_English be delivered?

Answer: Upon your order, we will start to translate GB/T 17999.9-2008_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 17999.9-2008_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 17999.9-2008_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GB/T 17999.9-2008?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 17999.9-2008 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.