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GB/T 17999.6-2008 English PDF

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Q: Should I purchase the latest version GB/T 17999.6-2008?

Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 17999.6-2008 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.

US$169.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 17999.6-2008: SPF chicken -- Microbiological surveillance -- Part 6: Enzyme-linked immunosorbent assay for SPF chicken
Status: Valid

GB/T 17999.6: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 17999.6-2008	169	Add to Cart	3 days	SPF chicken -- Microbiological surveillance -- Part 6: Enzyme-linked immunosorbent assay for SPF chicken	Valid
GB/T 17999.6-1999	199	Add to Cart	2 days	SPF chicken--Embryo susceptibility test	Obsolete

Similar standards

GB/T 25165 NY/T 1663 GB/T 20365 GB/T 17999.2 GB/T 17999.3 GB/T 17999.1

Basic data

Standard ID: GB/T 17999.6-2008 (GB/T17999.6-2008)
Description (Translated English): SPF chicken -- Microbiological surveillance -- Part 6: Enzyme-linked immunosorbent assay for SPF chicken
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 7,732
Date of Issue: 2008-12-31
Date of Implementation: 2009-05-01
Older Standard (superseded by this standard): GB/T 17999.5-1999
Regulation (derived from): National Standard Approval Announcement 2008 No.27 (Total No.140)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the technical requirements of the enzyme-linked immunosorbent assay. Indirect ELISA This section applies to the SPF chicken serum antibody detection of these pathogenic microorganisms: Haemophilus paragallinarum (Haemophilus), Mycoplasma gallisepticum (Mycoplasma gallisepticum), Mycoplasma synoviae (Mycoplasma synoviae), bird flu virus (Avian Influeza Virus), NDV (Newcastle Disease Virus), infectious bronchitis virus (Infectious Bronchitis Virus), infectious law 's.

GB/T 17999.6-2008: SPF chicken -- Microbiological surveillance -- Part 6: Enzyme-linked immunosorbent assay for SPF chicken

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
SPF chicken Microbiological surveillance Part 6. Enzyme-linked immunosorbent assay for SPF chicken ICS 11.220 B41 National Standards of People's Republic of China Replacing GB/T 17999.5-1999 SPF chickens microbiological monitoring Part 6. SPF chickens ELISA Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 17999 "SPF chickens microbiological monitoring" is divided into 10 parts. --- The first part 1. SPF chicken microbiological monitoring General; Part --- Article 2. SPF chicken hemagglutination inhibition test; Part --- Section 3. SPF chicken serum neutralization test; Part --- Chapter 4. SPF chicken serum plate agglutination test; Part --- Article 5. SPF chicken agar diffusion test; --- Part 6. SPF chickens ELISA; Part --- Article 7. SPF chicken embryo susceptibility testing; Part --- Article 8. SPF chicken Salmonella testing; --- Part 9. SPF chickens tube agglutination test; Part --- Article 10. SPF chickens indirect immunofluorescence assay. This section GB/T 17999 Part 6. The partial revision of the reference to GB/T 18936-2003 "highly pathogenic avian influenza diagnostic techniques", GB/T 19167-2003 "infectious bursal Disease Diagnosis ", NY/T 538-2002" infectious coryza diagnostic techniques ", OIE" terrestrial animals (mammals, birds and bees) diagnosis Manual of Tests and Vaccines "the relevant provisions (fifth edition) of. This Part replaces GB/T 17999.5-1999 "SPF chickens ELISA." This section compared with the GB/T 17999.5-1999 The main changes are as follows. --- Increased indirect enzyme-linked immunosorbent assay; --- Added Appendix A "reagent preparation." This section of Appendix A normative appendix. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Animal Epidemic Prevention Standardization Technical Committee (SAC/TC181) centralized. This section is drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China Animal Health and Epidemiology Center, Jinan Spa law Adams Poultry Limited. The main drafters of this section. song even East, Jiang Qian, Han Lingxia, satellite Shao, Zhu fruit, single Zhong Fang, Liu Jiasen Secretary Chand, Guodong Chun, Yu Haibo, Mengqing Wen. This part of the standard replaces the previous editions are. --- GB/T 17999.5-1999. SPF chickens microbiological monitoring Part 6. SPF chickens ELISA

1 Scope

This section GB/T 17999 specifies the technical requirements ELISA test. Indirect ELISA This section applies to SPF chicken serum antibody detection of the following pathogens. Haemophilus paragallinarum Avian influenza virus (AvianInfluezaVirus), Newcastle disease virus (NewcastleDiseaseVirus), infectious bronchitis virus (InfectiousBronchitisVirus), infectious bursal disease virus (InfectiousBursalDiseaseVirus), reticuloendothelial hyperplasia Virus (ReticuloendotheliosisVirus), chicken infectious anemia virus (ChickenInfectiousAnaemiaVirus), avian reovirus Virus (viral arthritis) (AvianReovirus), avian (AvianEncephalomyelitisVirus), infectious laryngotracheitis gas Tube stomatitis virus (InfectiousLaryngotracheitisVirus), avian adenovirus Ⅲ group (EDS) (AvianAdenovirusGroupⅢ), shower Ba-cell leukemia virus (LymphoidLeukosisVirus). This section double-antibody sandwich enzyme-linked immunosorbent assay suitable for SPF chickens lymphoblastic leukemia virus P27 antigen detection. Principle 2 Indirect enzyme-linked immunosorbent assay using known microbial antigens to detect unknown antibody, double antibody sandwich enzyme-linked immunosorbent assay has been adopted Known unknown antigen antibody detection.

3 indirect ELISA

3.1 Reagents 3.1.1 The envelope antigen negative and positive control sera, goat anti-chicken LgG HRP, saved and used according to instructions. 3.1.2 Appendix coating liquid diluent, substrate solution, stop solution preparation method A. 3.2 Equipment Microtiter plates, pipettes, 37 ℃ incubator, microplate reader. 3.3 Procedure 3.3.1 antigen-coated. the bird flu virus, infectious bronchitis virus, infectious bursal disease virus, infectious laryngotracheitis virus, new City disease virus, Haemophilus paragallinarum, avian adenovirus Ⅲ group (EDS), Mycoplasma gallisepticum, Mycoplasma synoviae, Avian encephalomyelitis virus, lymphocytic Leukemia virus, reticuloendothelial hyperplasia virus, avian reovirus (viral arthritis), chicken infectious anemia virus antigen coating buffer dilute Diluted to working concentration, was added to each well microtiter plates, each well 100μL, at 4 ℃ refrigerator overnight. 3.3.2 washing. rejection net hole antigen solution, with the washing liquid to fill the holes, place 5min, then dumped a net, so to repeat three times. 3.3.3 was tested serum plus. The dilution will be subject to 100 dilution of serum added to each well 100μL, each operation are set to negative control Two holes, the positive control wells, blank comparison wells each, were added to the same negative serum dilution of positive serum and dilutions of each 100μL, Plus different serum samples should change the tip, 37 ℃ incubator role 30min. 3.3.4 Washing. with 3.3.2. 3.3.5 plus goat anti-chicken IgG-HRP. The dilution of the enzyme-labeled antibody diluted to working concentration added to each well 100μL, 37 ℃ incubator The role of 30min. 3.3.6 Washing. with 3.3.2. 3.3.7 color. the addition of solution 100μL, dark at room temperature for about 5min reaction (negative control wells start to produce a color when). ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 17999.6-2008_English be delivered?

Answer: Upon your order, we will start to translate GB/T 17999.6-2008_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 17999.6-2008_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 17999.6-2008_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GB/T 17999.6-2008?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 17999.6-2008 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.