GB/T 17528-2009 English PDF

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GB/T 17528-2009: Determination of pipeline content -- Method using HPLC
Status: Valid

GB/T 17528: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 17528-2009	139	Add to Cart	3 days	Determination of pipeline content -- Method using HPLC	Valid
GB/T 17528-1998	239	Add to Cart	2 days	Determination of piperine content--Spectrophotometric method	Obsolete

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Basic data

Standard ID: GB/T 17528-2009 (GB/T17528-2009)
Description (Translated English): Determination of pipeline content -- Method using HPLC
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X14
Classification of International Standard: 67.200.10
Word Count Estimation: 6,688
Date of Issue: 2009-04-03
Date of Implementation: 2009-09-01
Older Standard (superseded by this standard): GB/T 17528-1998
Adopted Standard: ISO 11027-1993, MOD
Regulation (derived from): National Standard Approval Announcement 2009 No.5 (Total No.145)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the method for the determination of piperine content by HPLC. This standard applies to black, white pepper and black, white pepper and pepper oleoresin extraction alkali content of the product was measured. This standard method detection limit of 0. 008 g/100 g.

GB/T 17528-2009: Determination of pipeline content -- Method using HPLC

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of piperine content. Method using HPLC ICS 67.200.10 X14 National Standards of People's Republic of China Replacing GB/T 17528-1998 Pepper alkali content by HPLC (ISO 11027.1993, Pepperandpepperoleoresins-Determination chromatography, MOD) Posted 2009-04-03 2009-09-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This revised standard adopts ISO 11027.1993 "pepper and pepper oleoresin pepper alkali content by HPLC" This standard and ISO 11027.1993 main differences are as follows. --- The ISO 11027.1993 the name changed to Determinationofpiperinecontent-Methodusinghigh-per- --- Increasing the detection limit; --- The ISO 11027.1993 in a concentration range of 6.2 piperine standard solution "0.05g/L ~ 0.2g/L" changed to "0.4mg/L ~ 2.0mg/L "; --- The ISO 11027.1993 in 4.3 mobile phase "of acetonitrile and 1% acetic acid (48 + 52)" to "methanol - water (77 + 23)"; --- Cancellation ISO 11027.1993 10.1 10.2 formulas and calculations factor, because no formula to calculate the revised factor. This standard replaces GB/T 17528-1998 "Determination pepper alkali content spectrophotometry." This standard and GB/T 17528-1998 compared to major differences are. --- The spectrophotometric method to the High Performance Liquid Chromatography. Appendix A of this standard is an informative annex. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the national spice Standardization Technical Committee. This standard was drafted. the Ministry of Agriculture Food Quality Supervision and Testing Center (Zhanjiang). The main drafters of this standard. HEALTH LABORATORY Cheng Shenghua, Li Zhen Lin, Zhou Huiling, Lin Ling, Yu Bing search. This standard was first released in October 1998. Pepper alkali content by HPLC

1 Scope

This standard specifies the method for determining pepper alkali content by high performance liquid chromatography. This standard applies to black, white and black pepper, white pepper and pepper oleoresin extraction product Content Determination. The standard method detection limit of 0.008g/100g. Principle 2 The sample was extracted with ethanol piperine detected by HPLC UV detector, external standard.

3 Reagents

Unless otherwise indicated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity. 3.1 Ethanol (CH3CH2OH). 95% (mass fraction). 3.2 methanol (CH3OH). chromatography. 3.3 piperine standard substance. purity ≥98%. Piperine 3.4 Standard solution. Accurately weigh (10 ± 0.1) mg piperine standard brown substance in 10mL beaker, ethanol (3.1) was dissolved Solution, transferred to a 10mL brown volumetric flask with ethanol (3.1) to the mark, the concentration of the solution was 1000mg/L (when used with temporary system). 3.5 piperine standard working solution. lessons 50μL piperine standard solution (3.4) with a micropipette placed in 25mL brown volumetric flask, Ethanol (3.1) diluted to the mark, the concentration of the solution is 2.00mg/L.

4 Instrument

4.1 High performance liquid chromatograph. equipped with a UV detector. 4.2 Organization mashed machines. 4.3 Sample screens. a pore size of 500μm. 4.4 brown round bottom flask. 100mL, supporting reflux condenser apparatus. 4.5 brown volumetric flask. 10mL, 25mL, 100mL.

5 Sample Preparation

5.1 Whole pepper With a stamp mill (4.2) the sample is crushed, until all the samples through the sieve (4.3), in a brown bottle storage spare. 5.2 pepper All samples through the sample screen (4.3), not through a screen, with the masher (4.2) pulverized until the particle size to meet the requirements so far, in the storage Brown bottle aside. 5.3 pepper oleoresin The sample was sufficiently uniform. Step 6 Analysis Note. Because piperine solution is unstable, easily decomposed in light, dark operation is necessary. Operation with black paper or aluminum foil and the flask flask wrapped up, and Determination as soon as possible. ......

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