Home Cart Quotation About-Us
www.ChineseStandard.net
SEARCH

GB/T 15805.5-2018 English PDF

Q: How long will the true-PDF of English version of GB/T 15805.5-2018 be delivered?

Upon your order, we will start to translate GB/T 15805.5-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Q: Can I share the purchased PDF of GB/T 15805.5-2018_English with my colleagues?

Yes. The purchased PDF of GB/T 15805.5-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Q: Does the price include tax/VAT?

Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries). Avoidance of Double Taxation Agreements (DTAs) between Singapore and 100+ Countries: https://www.iras.gov.sg/taxes/international-tax

Q: Do you accept my currency other than USD?

Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Q: Should I purchase the latest version GB/T 15805.5-2018?

Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 15805.5-2018 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.

US$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 15805.5-2018: Protocol of diagnosis for spring viraemia of carp
Status: Valid

GB/T 15805.5: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 15805.5-2018	279	Add to Cart	3 days	Protocol of diagnosis for spring viraemia of carp	Valid
GB/T 15805.5-2008	319	Add to Cart	3 days	The quarantine methods of fish -- Part 5: Spring viraemia of carp virus (SVCV)	Obsolete

Similar standards

GB/T 25165 NY/T 1663 GB/T 20365 GB/T 15805.4 GB/T 15805.3 GB/T 15805.2

Basic data

Standard ID: GB/T 15805.5-2018 (GB/T15805.5-2018)
Description (Translated English): Protocol of diagnosis for spring viraemia of carp
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 65.020.30
Word Count Estimation: 14,188
Date of Issue: 2018-12-28
Date of Implementation: 2019-07-01
Older Standard (superseded by this standard): GB/T 15805.5-2008
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 15805.5-2018: Protocol of diagnosis for spring viraemia of carp

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of diagnosis for spring viraemia of carp ICS 65.020.30 B41 National Standards of People's Republic of China Replace GB/T 15805.5-2008 Hunchun viremia diagnosis procedure Published on.2018-12-28 2019-07-01 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces GB/T 15805.5-2008 "Fish quarantine method Part 5. Hunchun viremia virus (SVCV)". versus Compared with GB/T 15805.5-2008, except for editorial changes, the main technical changes are as follows. --- Added abbreviations; --- Added enzyme-linked immunosorbent assay (ELISA) to detect hunchun viremia Method of antigen (springviraemiaofcarpvirus, SVCV); ---Incremental indirect immunofluorescent antibody test (IFAT) to detect SVCV Method of antigen; --- Added real-time quantitative PCR (real-time PCR) method for detecting SVCV nucleic acid; --- Increased the comprehensive judgment of springviraemiaofcarp (SVC). Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic This standard is under the jurisdiction of the National Aquaculture Standardization Technical Committee (SAC/TC156). This standard was drafted. Ningbo Ocean and Fisheries Research Institute, National Aquatic Technology Extension Station, Beijing Customs, People's Republic of China, China Shekou Customs of the People's Republic of China. The main drafters of this standard. Wang Jianping, Zhang Lifeng, Jiang Yulin, Xu Shengwei, Gao Longying, Wu Xiongfei, Chen Aiping, Shen Weiliang. The previous versions of the standards replaced by this standard are. ---GB/T 15805.1-1995; ---GB/T 15805.5-2008. Hunchun viremia diagnosis procedure

1 Scope

This standard specifies the sampling of viremia viremia, virus isolation and culture, RT-PCR, real-time quantitative RT-PCR, enzyme-linked immunosorbent assay Test, indirect immunofluorescent antibody test, comprehensive determination method. This standard applies to the epidemiological investigation, diagnosis, quarantine and epidemic monitoring of Hunchun viremia.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods SC/T 7103 Technical specification for quarantine sampling of aquatic animals

3 Abbreviations

The following abbreviations apply to this document. CO. grass carp ovary cell line (grasscarpovarycelline) CPE. cytopathic effect EB. ethidium bromide (ethidiumbromide) ELISA. enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay) EPC. epithelioma cell line (epitheliomapapulosumcyprinicelline) FCS. fetal calf serum (fetalcalfserum) FHM. fat head muscle cell line (fatheadmonnowcelline) FITC. fluorescein isothiocyanate G protein. glycoprotein IFAT. indirect immunofluorescent antibody test (indirectimmunofluorescentantibodytest) RT-PCR. reverse transcription-polymerase chain reaction (reverse transcription polymerase chain reaction) SVC. spring viremia (springviraemiaofcarp) SVCV. springviraemiaofcarpvirus

4 reagents and materials

4.1 Water. Meet the specifications of the first grade water in GB/T 6682; nucleic acid extraction and RT-PCR should use RNase-free deionized water. 4.2 SVCV reference strain. provided by the designated unit of the Ministry of Agriculture and Rural Areas of the People's Republic of China. 4.3 Fish cell lines. EPC, FHM, CO. 4.4 Fish cell growth solution. Prepared with Earle's balanced salt in M199 medium plus 10% fetal bovine serum (FCS). 4.5 Fish cell maintenance solution. Prepared with Earle's balanced salt in M199 medium plus 2 S. 4.6 Cell Digestion. See Appendix A, A.1. ......

Image

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 15805.5-2018_English be delivered?

Answer: Upon your order, we will start to translate GB/T 15805.5-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 15805.5-2018_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 15805.5-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GB/T 15805.5-2018?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 15805.5-2018 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.