GB 28404-2012 English PDFUS$229.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB 28404-2012: National food safety standards in health food α-linolenic acid, eicosapentaenoic acid, docosapentaenoic acid and 22 carbon 60-acid determination Status: Valid
Basic dataStandard ID: GB 28404-2012 (GB28404-2012)Description (Translated English): National food safety standards in health food ��-linolenic acid, eicosapentaenoic acid, docosapentaenoic acid and 22 carbon 60-acid determination Sector / Industry: National Standard Classification of Chinese Standard: C54;X40 Classification of International Standard: 67.220.20 Word Count Estimation: 10,153 Regulation (derived from): Ministry of Health Bulletin 2012 No. 9 Issuing agency(ies): Ministry of Health of the People's Republic of China Summary: This Chinese standard specifies the health food ��- linolenic acid, eicosapentaenoic acid (referred to EPA, the same below), docosapentaenoic acid (abbreviated DPA, the same below) and docosahexaenoic acid (abbreviated DHA, the same below) of the gas chrom GB 28404-2012: National food safety standards in health food α-linolenic acid, eicosapentaenoic acid, docosapentaenoic acid and 22 carbon 60-acid determination---This is a DRAFT version for illustration, not a final translation. 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National food safety standards in health food α-linolenic acid, eicosapentaenoic acid, docosapentaenoic acid and 22 carbon 60-acid determination National Standards of People's Republic of China National standards for food safety Health food in the α-linolenic acid, eicosapentaenoic acid, Docosahexaenoic acid and docosahexaenoic acid Of the determination 2012-05-17 release 2012-07-17 Implementation Issued by the Ministry of Health of the People's Republic of China National standards for food safety Health food in the α-linolenic acid, eicosapentaenoic acid, Docosahexaenoic acid and docosahexaenoic acid Of the determination 1 ScopeThis standard specifies the health food α-linolenic acid, eicosapentaenoic acid (referred to as EPA, the same below), docosahexaenoic acid (referred to as DPA, the same below) and docosahexaenoic acid (hereinafter referred to as DHA, the same below) by gas chromatography. This standard is applicable to the determination of α-linolenic acid, EPA, DPA and DHA in health food products, and is not suitable for the determination of fatty acid Determination of α-linolenic acid, EPA, DPA, DHA in health food.2 principleThe samples were extracted by acid hydrolysis to extract fats, including alpha-linolenic acid, eicosapentaenoic acid (EPA), docosahexaenoic acid (DPA), twenty-two Carboxyhexaenoic acid (DHA) was transesterified to form methyl ester, which was separated by gas chromatography to determine the time qualitative and external standard method.3 reagents and materialsNote. Unless otherwise stated, the reagents used in this method are of analytical grade and water is a primary water specified in GB/T 6682. 3.1 Reagents 3.1.1 Potassium hydroxide (KOH). 3.1.2 hydrochloric acid (HCl). 3.1.3 anhydrous ether (C2H5OC2H5). 3.1.4 Ethanol (CH3CH2OH). volume fraction ≥ 95%. 3.1.5 petroleum ether. boiling range 30 ℃ ~ 60 ℃. 3.1.6 n-Hexane [CH3 (CH2) 4CH3]. Chromatographic Purification. 3.1.7 Methanol (CH3OH). Chromatographic Purification. 3.1.8 anhydrous sodium sulfate (Na2SO4). 3.2 reagent preparation Potassium hydroxide solution of methanol (0.5mol/L). Weigh 2.8g potassium hydroxide, dissolved in methanol and volume to 100mL, and mix. 3.3 standards 3.3.1 α-linolenic acid methyl ester (C19H32O2). purity ≥ 99.0%. 3.3.2 EPA methyl ester (C21H32O2). purity ≥ 98.5%. 3.3.3 DPA methyl ester (C23H36O2). purity ≥ 98.0%. 3.3.4 DHA methyl ester (C23H34O2). purity ≥ 98.5%. 3.4 Preparation of standard solution 3.4.1 Single Fatty Acid Methyl Ester Standard Stock Solution (4.0 mg/mL). Weigh 100.0 mg alpha-linolenic acid methyl ester, EPA methyl ester, DPA A Ester, DHA methyl ester standard material in 25.0mL volumetric flask, respectively, with n-hexane dissolved and fixed to the scale, shake. This solution should be stored In -18 ℃ refrigerator. 3.4.2 Fatty Acid Methyl Ester Mix Standard Intermediate (1.0mg/mL). Separate Fatty Acid Methyl Ester Standard Stock 2.50mL in 10.0mL Capacity bottle, shake, but also for the highest standard curve of the standard, when the preparation of temporary use. 3.4.3 fatty acid methyl ester standard working solution. respectively, to absorb fatty acid methyl ester intermediate 0.40mL, 0.80mL, 1.0mL, 2.0mL, 4.0 mL in a 10 mL volumetric flask, with a volume of 0.040 mg/mL, 0.080 mg/mL, 0.10 mg/mL, 0.20mg/mL, 0.40mg/mL standard working solution, when used in preparation.4 instruments and equipment4.1 Gas Chromatograph. with a hydrogen flame ionization detector (FID). 4.2 balance. sense of 1mg and 0.1mg. 4.3 Rotary Evaporator. 4.4 Centrifuge. speed ≥ 4000r/min. 4.5 Scroll Mixer. 4.6 constant temperature water bath.5 Analysis steps5.1 Preparation of the sample 5.1.1 Sample handling 5.1.1.1 Solid samples Weigh the pulverized and mixed samples to be tested 0.5g ~ 2g (accurate to 0.001g) (including the measured components 5mg/g ~ 10mg/g) plus Into the 50mL colorimetric tube, add 8mL water, mix and add 10mL hydrochloric acid. The color tube into the 70 ℃ ~ 80 ℃ water bath, every time 5min ~ 10min with a vortex mixer mixed once, until the sample hydrolysis is complete, about 40min ~ 50min. Remove the color tube, add Into 10mL of ethanol, mixed. After cooling to room temperature, the mixture was transferred to a 100 mL stopper cylinder, washed with 25 mL of anhydrous ether Color tube, into the cylinder together. Dense plug shaking 1min. Add 25mL petroleum ether, dense plug shaking 1min, standing 30min, stratified, will The extracted organic layer was passed through an anhydrous sodium sulfate (about 5 g) into a concentration flask. Then add 25mL anhydrous ether dense plug shaking 1min, 25mL Petroleum ether, dense plug shaking 1min, standing, stratified, the aspirated organic layer through anhydrous sodium sulfate (about 5g) into the concentration of the bottle. Press "plus Into 25 mL of anhydrous ether, let stand, layer, the organic layer of the suction through anhydrous sodium sulfate (about 5g) filter into the bottle "repeat operation At one time, the whole extract was concentrated to 45 ° C under a rotary evaporator at reduced pressure to near dryness. Dissolve the concentrate a few times with n-hexane and transfer to 25mL volumetric flask and volume, shake. Methylation in accordance with 5.1.2. 5.1.1.2 Oil products Weigh the mixed oil products 0.2g ~ 1g (accurate to 0.001g) (including the measured components 10mg/g ~ 20mg/g) to 25mL Capacity flask, add 5mL n-hexane shake to dissolve, and n-hexane volume to the mark, shake. According to 5.1.2 step methyl esterification treatment, fat For the physical identification of ethyl ethyl ester type oils, see Appendix A. 5.1.2 Methylation Absorb the test solution (5.1.1.1, 5.1.1.1) 2.0mL to 10mL with a plug scale tube, add 2.0mL potassium hydroxide methanol solution Liquid, immediately moved to the vortex mixer for 5min oscillation, put it aside for 5min, add 6mL distilled water, shaking up and down for 0.5min, Layer, the lower layer of liquid, discard and then repeated with a small amount of distilled water for washing, and with a straw to abandon the water layer until the wash to neutral (if organic Phase with the phenomenon of emulsification to 4000r/min centrifugal 10min), the absorption of n-hexane to be on the machine test. Note. If the use of plastic centrifuge tube or plastic scale tube for sample processing should be synchronized to the blank control test. 5.2 Gas Chromatographic Reference Conditions 5.2.1 Column. Bonded cross-linked polyethylene glycol stationary phase, column length 30m, inner diameter 0.32mm, film thickness 0.5μm or equivalent Column. 5.2.2 oven temperature. the initial temperature of 180 ℃, 10 ℃/min temperature to 220 ℃, and then 8 ℃/min temperature to 250 ℃, to maintain 13min. And Rhithers. and Rhithers. Find Results Results Results (Rhithers. Results.1a ... Results Resultss. 5.2.4 FID detector temperature. 270 ℃. And Rhithers. Refinding polymers Results. Results and round - And Rhithers. Refinding polymers Results. Results Resultss. Rhithertulates Results Results Results All nightss Rhithers. Refinding hearts Results Rhitherical calling Results 5.3 Production of standard curve Add 1 μL of the standard series of each concentration solution (3.4.2, 3.4.3) into the gas chromatograph and measure the corresponding peak area or peak height The standard working fluid concentration is the abscissa, with the peak area or peak height as the ordinate, draw the standard curve (standard solution gas chromatogram see Appendix B Figure B.1). 5.4 Determination of test solution 1 μL of the test solution (5.1.2) was injected into the gas chromatograph to retain the time qualitative, measured peak area or peak height, according to the standard The curve gives the concentration of the components of the fatty acid methyl ester in the test solution (see Figure B.2 in Appendix B for gas chromatograms of the sample solution).6 Analysis of the results of the expressionThe contents of α-linolenic acid, EPA, DPA and DHA in the sample were calculated according to formula (1) Xi = ci × V × F × 100m × 1000 (1) Where. Xi --- The content of α-linolenic acid, EPA, DPA, DHA in grams, in grams per gram (g/100g); ci --- from the standard curve to determine the determination of the concentration of each fatty acid methyl ester in milligrams per milliliter (mg/mL); V --- the final volume of the sample to be measured, in milliliters (mL); m --- sample sample quality, in grams (g); F --- conversion factor of each fatty acid methyl ester into fatty acid, wherein. α-linolenic acid methyl ester is converted into α-linolenic acid conversion system The number of 0.9520; EPA methyl ester conversion to EPA fatty acid conversion coefficient of 0.9557; DPA methyl ester into DPA fatty acid conversion coefficient of 0.9592; DHA methyl ester into DHA fatty acid conversion coefficient of 0.9590; 100 --- unit conversion; 1000 --- unit conversion. The calculated results are expressed as the arithmetic mean of the two independent determinations obtained under repeated conditions, leaving two significant digits.7 precisionThe absolute difference between the two independent determinations obtained under repeatability does not exceed 10% of the arithmetic mean.8 otherWhen the sample volume is 0.5g, the volume of constant volume is 25mL, the limit of each fatty acid is α-linolenic acid 0.010g/100g, EPA 0.018 g/100 g, DPA0.024 g/100 g, DHA 0.018 g/100 g.Appendix APhysical identification of fatty acid ethyl ester oil products Weigh 1 g of the sample, add 1.0 mL of absolute ethanol, in a vortex mixer (2000r/min) for 30s, standing, observe the oil in ethanol In the dissolution of the situation. If the oil is insoluble in ethanol, there is a clear phenomenon of oil and ethanol stratification, then determine this product is fatty acid glyceride Type oil products can be used to analyze this standard; if standing, can not see the oil-like droplets, dissolved completely and the solution is clear and transparent, you can determine this The product is a fatty acid ethyl ester type oil product and can not be analyzed using this standard.Appendix BStandard solution and sample solution Typical gas chromatogram B.1 α-linolenic acid methyl ester, EPA methyl ester, DPA methyl ester, DHA methyl ester standard solution chromatogram, see Figure B.1. 1 --- α-linolenic acid methyl ester; 2 --- EPA methyl ester; 3 --- DPA methyl ester; 4 --- DHA methyl ester. Figure B.1 α-linolenic acid methyl ester, EPA methyl ester, DPA methyl ester, DHA methyl ester standard solution chromatogram B.2 A chromatogram of a sample solution containing methyl α-linolate, EPA methyl ester, DPA methyl ester, DHA methyl ester, see Figure B.2. 1 --- α-linolenic acid methyl ester; 2 --- EPA methyl ester; 3 --- DPA methyl ester; 4 --- DHA methyl ester. Figure B.2 Chromatogram of sample solution containing methyl α-linolate, EPA methyl ester, DPA methyl ester, DHA methyl ester ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB 28404-2012_English be delivered?Answer: Upon your order, we will start to translate GB 28404-2012_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB 28404-2012_English with my colleagues?Answer: Yes. 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