GB 23200.113-2018 English PDF

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GB 23200.113-2018: National food safety standard -- Determination of 208 pesticides and their metabolite residues in plant-derived foods -- Gas chromatography -- Mass spectrometry
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB 23200.113-2018	989	Add to Cart	10 days	National food safety standard -- Determination of 208 pesticides and their metabolite residues in plant-derived foods -- Gas chromatography -- Mass spectrometry	Valid

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GB/T 38211   GB/T 18418   GB/T 18419   GB 23200.120   GB 23200.121   GB 23200.119   

Basic data

Standard ID: GB 23200.113-2018 (GB23200.113-2018)
Description (Translated English): National food safety standard -- Determination of 208 pesticides and their metabolite residues in plant-derived foods -- Gas chromatography -- Mass spectrometry
Sector / Industry: National Standard
Classification of Chinese Standard: G25
Word Count Estimation: 46,477
Date of Issue: 2018-06-21
Date of Implementation: 2018-12-21
Regulation (derived from): National Health and Wellness Commission Announcement No.6 of 2018
Issuing agency(ies): National Health Commission of the People's Republic of China, State Administration for Market Regulation

GB 23200.113-2018: National food safety standard -- Determination of 208 pesticides and their metabolite residues in plant-derived foods -- Gas chromatography -- Mass spectrometry

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National Standards of People's Republic of China National food safety standards 208 pesticides and their metabolites in plant-derived foods Determination of residual amounts Gas Chromatography-Mass Spectrometry State Administration for Market Regulation Ministry of Agriculture and Rural Affairs of the People's Republic of China National Health Commission of the People's Republic of China

1 Scope

This standard specifies a gas chromatography-mass spectrometry method for the determination of 208 pesticides and their metabolites (see Appendix A) in plant-derived foods: This standard is applicable to the determination of 208 pesticides and their metabolites residues in plant-derived foods:

2 Normative reference documents

The following documents are essential for the application of this document: For dated application documents, only the dated version applies to this document: For undated referenced documents, the latest version (including all amendments) applies to this document: GB 2763 National Food Safety Standard Maximum Residue Limits of Pesticides in Foods GB/T 6682 Specifications and test methods for water used in analytical laboratories

3 principles

The sample is extracted with acetonitrile, and the extract is purified by solid-phase extraction or dispersive solid-phase extraction: The vegetable oil sample is purified by gel permeation chromatography, detected by gas chromatography-mass spectrometry, and quantified by the internal standard method or external standard method:

4 Reagents and materials

Unless otherwise stated, only analytically pure reagents are used in the analysis, and the water is first-grade water specified in GB/T 6682: 4:1 Reagents 4:1:1 Acetonitrile (CH₃CN, CAS number: 75-05-8): 4:1:2 Ethyl acetate (CH₃COOC₂Hs, CAS number: 141-78-6): chromatographically pure: 4:1:3 Toluene (C₇Hg, CAS number: 108-88-3): chromatographically pure: 4:1:4 Cyclohexane (C₆Hi, CAS number: 110-82-7): chromatographically pure: 4:1:5 Sodium chloride (NaCI, CAS number: 7647-14-5): 4:1:6 Sodium acetate (CH₃COONa, CAS number: 6131-90-4): 4:1:7 Acetic acid (CH₃COOH, CAS number: 55896-93-0): 4:1:8 Magnesium sulfate (MgSO₄, CAS number: 7487-88-9): 4:1:9 Sodium citrate (Na₃C₆H₅O₂, CAS number: 6132-04-3): 4:1:10 Disodium hydrogen citrate (C₆H₆Na₂O₂, CAS number: 6132-05-4): 4:2 Solution preparation 4:2:1 Acetonitrile-acetic acid solution (99 1, volume ratio): Measure 10 mL acetic acid and add it to 990 mL acetonitrile, and mix well: 4:2:2 Acetonitrile-toluene solution (3 1, volume ratio): Measure 100 mL of toluene and add it to 300 mL of acetonitrile, and mix well: 4:2:3 GPC mobile phase: cyclohexane-ethyl acetate solution (1 1, volume ratio): Measure 500 mL of cyclohexane and add it to 500 mL of ethyl acetate, and mix well: 4:3 Standard products Heptachlor epoxide B internal standard and 208 pesticides and their metabolite standards, see Appendix A, purity ≥95%: 4:4 Preparation of standard solution 4:4:1 Standard stock solution (1000mg/L): Accurately weigh 10mg (accurate to 0:1mg) of each pesticide standard, dissolve it in a solvent such as acetone or n-hexane according to the solubility of the standard and the needs of the measurement, and dilute to 10mL , Store away from light at -18℃, valid for 1 year: 4:4:2 Mixed standard solution (Mixed standard solution A and B): According to the properties and retention time of the pesticides, 208 pesticides and their metabolites Divide into two groups, A and B: Pipette a certain amount of pesticide standard stock solution into a 250 mL volumetric flask, and dilute to the mark with ethyl acetate: The mixed standard solution should be stored at 0℃~4℃ away from light and is valid for 1 month: 4:4:3 Internal standard solution: Accurately weigh 10 mg of heptachlor B epoxide (accurate to 0:1 mg), dissolve it in ethyl acetate, transfer it to a 10 mL volumetric flask, adjust the volume and mix well to become the internal standard stock solution: The internal standard stock solution was diluted with ethyl acetate to 5 mg/L to become the internal standard solution: 4:4:4 Matrix mixed standard working solution: Blow dry the matrix solution with nitrogen, add 20 μL of internal standard solution, add 1 mL of mixed standard solution of corresponding mass concentration to reconstitute, and pass through a microporous filter membrane (4:5:6): The matrix mixed standard working solution should be prepared freshly: Note: The sampling volume of blank matrix solution should be consistent with the corresponding sample processing volume: 4:5 Materials 4:5:1 Solid phase extraction column: graphitized carbon black-amino composite column, 500mg/500mg, volume 6mL: 4:5:2 Ethylenediamine-N-propyl silanized silica gel (PSA): 40 μm ~ 60 μm: 4:5:3 Octadecylsilane bonded silica gel (Cig): 40 μm ~ 60 μm: 4:5:4 Graphitized carbon black (GCB): 40 μm ~ 120 μm: 4:5:5 Ceramic homogeneous proton: 2cm (length) × 1cm (outer diameter): 4:5:6 Microporous filter membrane (organic phase): 13mm×0:22μm:

5 instruments

5:1 Gas chromatograph-triple quadrupole mass spectrometer: equipped with electron impact source (ED): 5:2 Gel permeation chromatograph or device: equipped with 25 mm (inner diameter) × 500 mm, equipped with Bio-Beads SX-3 packing or equivalent purification column: 5:3 Analytical balance: sensitive to 0:1mg and 0:01g: 5:4 High-speed homogenizer: the rotation speed is not less than 15000 r/min: 5:5 Centrifuge: the rotation speed is not less than 4200 r/min: 5:6 Tissue masher: 5:7 Rotary evaporator: 7:2 Solid phase extraction pretreatment 7:2:1 Extraction 7:2:1:1 Vegetables, fruits and edible fungi Weigh 20g of sample (accurate to 0:01g) into a 100mL plastic centrifuge tube, add 40mL of acetonitrile, homogenize with a high-speed homogenizer at 15000r/min for 2 minutes, add 5g to 7g of sodium chloride, shake vigorously several times, and centrifuge at 4200r/min: 5 minutes: Accurately pipette 10mL of supernatant into a 100mL eggplant-shaped bottle: Rotate evaporate in a 40°C water bath to about 1 mL, blow with nitrogen until almost dry, and wait for purification: 7:2:1:2 Cereals, oils, nuts, tea and spices Weigh 5g of sample (accurate to 0:01g) into a 100mL plastic centrifuge tube, add 10mL of water, vortex and mix, and let stand for 30 minutes: Add 20 mL of acetonitrile, homogenize with a high-speed homogenizer at 15,000 r/min for 2 min, add 5 to 7 g of sodium chloride, shake vigorously several times, and centrifuge at 4,200 r/min for 5 min: Accurately transfer 5 mL of the supernatant into a 100 mL eggplant-shaped bottle, and rotary evaporate it to about 1 mL in a 40°C water bath: Blow it with nitrogen until it is almost dry and wait for purification: 7:2:2 Purification Prewash the solid phase extraction column (4:5:1) with 5 mL of acetonitrile-toluene solution (4:2:2) and discard the effluent: Connect the 150mL chicken heart bottle and place it on the fixed rack: Wash the above sample to be purified with 3 mL of acetonitrile-toluene solution (4:2:2) into the solid phase extraction column, and then use 2 mL of acetonitrile-toluene solution (4:2:2) to Wash with benzene solution (4:2:2), move the washing liquid into the column, and repeat twice: Add a 50 mL liquid reservoir to the column, rinse the column with 25 mL of acetonitrile-toluene solution, collect all the above effluents in a 150 mL chicken heart bottle, and spin and concentrate in a 40°C water bath until nearly dry: Add within 50μL Standard solution, add 2:5mL ethyl acetate to reconstitute, pass through a microporous filter membrane (4:5:6), and use it for measurement: ......

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