GB 1886.242-2016 English PDFUS$159.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB 1886.242-2016: National Food Safety Standard -- Food Additives -- Ammonium Glycyrrhetate Status: Valid
Basic dataStandard ID: GB 1886.242-2016 (GB1886.242-2016)Description (Translated English): National Food Safety Standard -- Food Additives -- Ammonium Glycyrrhetate Sector / Industry: National Standard Classification of Chinese Standard: X40 Word Count Estimation: 8,852 Date of Issue: 2016-08-31 Date of Implementation: 2017-01-01 Regulation (derived from): Announcement of the State Administration of Public Health and Family Planning 2016 No.11 Issuing agency(ies): National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration GB 1886.242-2016: National Food Safety Standard -- Food Additives -- Ammonium Glycyrrhetate---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.(Food safety national standard - Food additive - Ammonium glycyrrhizinate) National Standards of People's Republic of China National Food Safety Standard Licorice ammonium food additives Published 2016-08-31 2017-01-01 implementation People's Republic of China National Health and Family Planning Commission issued National Food Safety Standard Licorice ammonium food additives 1 ScopeThis standard applies to the raw material licorice (Glycyrrhiza legume GlycyrrhizauralensisFisch., Glycyrrhiza inflata Glycyrrhizain- flataBat. Glycyrrhiza glabra and GlycyrrhizaglabraL.), by Aqueous Extraction, sulfuric acid (or hydrochloric acid) the precipitate was acidified, ethanol extraction, amide, junction Crystal and other food additive prepared by the process ammonium glycyrrhizinate. 2 chemical name, formula, formula, relative molecular mass 2.1 Chemical Name Licorice ammonium 2.2 Structure Formula 2.3 C42H61O16NH4 2.4 Molecular Weight 839.96 (according to 2007 international relative atomic mass)3 Technical requirements3.1 Sensory requirements Sensory requirements shall comply with the requirements in Table 1. Table 1 Sensory requirements Project requires test methods Color white, yellowish white or pale yellow State of the fine particles powder Proper amount of sample is placed in a clean, dry white porcelain plate , Under natural light, the color observed and the state 3.2 Physical and Chemical Indicators Physical and chemical indicators should be consistent with the provisions of Table 2. Table 2. Physical and chemical indicators Item Index Test Method Ammonium glycyrrhizinate content (in terms 18β- ammonium glycyrrhizinate, dry basis), w /% ≥ 65.0 Appendix A A.4 Drying loss, w /% ≤ 7.0 Appendix A A.3 Specific rotation [α] 20 ° D/[(°) · mL · dm-1 · g-1] 45 ~ 53 in Appendix A A.5 Ash, w /% ≤ 0.5 A.6 Appendix A Lead (Pb)/(mg/kg) ≤ 2.0 Appendix A A.7 Total arsenic (As)/(mg/kg) ≤ 2.0 Appendix A A.8Appendix ATesting method A.1 General Provisions This standard reagents and water, in the absence of other requirements specified, refer to three analytical reagent water and GB/T 6682 provisions. test Test used in the standard solution, the standard solution impurities, products and preparations, in the absence of other requirements specified, are by GB/T 601, GB/T 602, GB/T 603 Preparation predetermined. The test does not indicate when the solution which is formulated with a solvent, refer to the aqueous solution. A.2 Identification Test A.2.1 Method summary After met alkali salts to produce ammonia, and volatile alkaline using ammonia wetting characteristics above enables the tube alkaline pH test paper color, Kam Do not salt presence. A.2.2 Reagents and materials Sodium hydroxide solution A.2.2.1. 40g/L. A.2.2.2 pH test paper. A.2.3 identification method Take a sample of about 0.2g ammonium glycyrrhizinate in 10mL test tube, adding about 1mL sodium hydroxide solution (A.2.2.1), shaken, which has been rapidly PH paper wetted with water placed in the tube mouth, and generating volatilized ammonia can wet pH paper color is alkaline. NOTE. The solution was heated, the color reaction is more obvious. Determination of loss on drying A.3 A.3.1 Instruments and Equipment A.3.1.1 vacuum drying oven. A.3.1.2 weighing bottle. A.3.1.3 dryer. Procedure A.3.2 The weighing bottle placed in a vacuum oven, 78 ℃ ± 0.5 ℃, 30min dried under 20mm Hg condition, taken out into the dryer The cooled to room temperature, and weighed. 1g sample was weighed to the nearest 0.0001g, stoppered, weighed. Gently shake the horizontal transverse weighing bottle, Uniformly distributed as a sample layer thickness of not more than 5mm. The plated sample weighing bottle placed in a vacuum oven, open the cork, in At 78 ℃ ± 0.5 ℃, 20mmHg conditions, dried under reduced pressure 6h, taken into the dryer, stoppered, allowed to cool to room temperature, and weighed. A.3.3 calculation results Loss on drying of the mass fraction w1, calculated according to formula (A.1). w1 = m1-m2 m × 100% (A.1) Where. --- M1 plus sample weighed bottle under reduced pressure before drying mass in grams (G); --- M2 plus sample weighed bottle under reduced pressure after drying mass in grams (G); --- m sample mass values, in units of grams (g). The results parallel arithmetic mean subject to measurement results (Reserved 1 decimal place). Obtained in two independent measurements under repeatability conditions Absolute difference determination result is not more than 1.0%. A.4 Determination of Ammonium glycyrrhizinate content (in terms 18β- ammonium glycyrrhizinate, dry basis) of A.4.1 Method summary Sample acetonitrile - aqueous solution of filtered, determined by liquid chromatography (UV detector), external standard. When the calculation results, the sample Weigh the sample by subtracting the mass of the sample mass of water, the result is a dry mass fraction yl (C42H65NO16) meter of ammonium glycyrrhizinate. A.4.2 Reagents and materials A.4.2.1 Acetonitrile. HPLC grade. A.4.2.2 glacial acetic acid. excellent pure. A.4.2.3 0.45μm membrane (organic phase). A.4.2.4 ammonium glycyrrhizinate standards. purity ≥90% (HPLC or identification). Note 1. ammonium glycyrrhizinate standards used in the process is a single component 18β- ammonium glycyrrhizinate standards. Note 2. Nonaqueous standard (NT) or other non-HPLC method for determination of the identification, which can not be directly used to calculate the value of the content liquid chromatography content. A.4.2.5 acetonitrile - aqueous solution. mixing a volume of acetonitrile and water. A.4.2.6 acetonitrile - acetic acid - solution. acetonitrile acetate ice water (38,161), mixed, and passed through 0.45μm filter (organic phase) Filtration, thoroughly degassed. A.4.3 Instruments A.4.3.1 HPLC equipped with UV detector. A.4.3.2 balance, a sense of the amount of 0.01mg. A.4.3.3 vacuum drying oven. A.4.4 Analysis step A.4.4.1 Standard Preparation Weigh 12.5mg ammonium glycyrrhizinate standard, accurate to 0.01 mg, placed in a 25mL volumetric flask with acetonitrile - aqueous solution (A.4.2.5) was dissolved Solution, and dilute to the mark. Filtered through a 0.45μm membrane (organic phase). Using now. Preparation of sample solution A.4.4.2 Weigh the sample 50.0mg ammonium glycyrrhizinate, accurate to 0.01 mg, placed in 100mL volumetric flask with acetonitrile - aqueous solution (A.4.2.5) was dissolved Solution to volume, filtered through a 0.45μm membrane (organic phase), to be tested. A.4.4.3 Reference chromatographic conditions A.4.4.3.1 Column. C18 column, length 150mm, internal diameter 4.6mm, particle size 5μm. Or equivalent chromatographic column separation effect. A.4.4.3.2 mobile phase. acetonitrile - acetic acid - solution (A.4.2.6). A.4.4.3.3 flow rate. 1.0mL/min. A.4.4.3.4 Column temperature. room temperature. A.4.4.3.5 Injection volume. 10μL. A.4.4.4 Determination Injection of sample solution and standard solution are sequentially ammonium glycyrrhizinate, measured at 254nm, external standard. High performance liquid ammonium licorice And the retention time of the chromatogram see Appendix B. A.4.5 calculation results Ammonium glycyrrhizinate content (in terms 18β- ammonium glycyrrhizinate, dry basis) the mass fraction w2, calculated according to equation (A.2). w2 = V × cs × A m3 × (1-w1) × As × 100% (A.2) Where. V --- sample constant volume in milliliters (mL); CS --- Ammonium glycyrrhizinate standard solution concentration in milligrams per milliliter (mg/mL); A --- sample solution chromatogram peak area of ammonium glycyrrhizinate; --- M3 sample quality value, in milligrams (mg); --- dried sample mass fraction W1 decrement,%; Ammonium glycyrrhizinate peak area of the standard solution --- AS chromatogram. The results parallel arithmetic mean subject to measurement results (Reserved 1 decimal place). Obtained in two independent measurements under repeatability conditions Absolute difference determination result is no more than 5% of the arithmetic mean. A.5 optical rotation measured ratio A.5.1 Reagents and materials A.5.1.1 ethanol. A.5.1.2 40% ethanol - solution. Take 2 parts of ethanol and 3 parts water were mixed. A.5.2 Instruments and Equipment Optical photometer, minimum scale measurement 0.05 °. A.5.3 Analysis step Preparation of sample solution A.5.3.1 1.5 g was weighed (accurate to 0.01g) of the sample is not dried, dissolved in 100mL of ethanol - solution (A.5.1.2) in the test. A.5.3.2 Instrument Conditions A.5.3.2.1 measurement wavelength. sodium lamp D-line (589.3nm). A.5.3.2.2 measured temperature. ± 0.5 ℃ 20 ℃. A.5.3.3 Determination After the instrument is turned on stability, with ethanol - solution (A.5.1.2) as a blank solution, zero calibration of the instrument (not auto-zeroing the instrument, to be Blank value was measured, deduction blank), test solution is filled with clean, dry optical tube, carefully remove air bubbles, the instrument by screwing the cap described Book operation, measured at a solution temperature condition of the sample 20 ℃ ± 0.5 ℃, optical rotation is read. Zuo Xuan minus sign "-" indicates, D-positive No. "" indicates. A.5.4 calculation results To specific rotation [α] 20 ° D represents a numerical order "(°) · mL · dm-1 · g-1" basis, according to equation (A.3) is calculated. [Α] 20 ° D = α × 100 l × (m4-m4 × w1) (A.3) Where. --- [alpha] of the sample solution measured optical rotation in degrees ([deg.]); Of ethanol was dissolved 100 --- ammonium glycyrrhizinate sample - volume of the aqueous solution, in milliliters (mL); L --- optical tube length in decimeters (DM); --- M4 said ammonium glycyrrhizinate sample quality taken in grams (G); --- dried sample mass W1 fraction reductions,%. The arithmetic average of three measurement results taken parallel to the measurement result to an integer reserved bits. A.6 Determination of ash A.6.1 Instruments and Equipment High temperature furnace. A.6.2 Analysis step 3g sample was weighed (accurate to 0.0001g), was placed in 100mL constant weight porcelain crucible, Fo Tan of small non-through. Move 550 ℃ ± 25 ℃ A high temperature furnace burning until the sample is completely ash (approximately 3H), cooled to room temperature in a desiccator, weighed, repeated ignition until constant weight (referred to the two front The difference between the amount does not exceed 0.5mg). A.6.3 calculation results Ash content w3, calculated according to formula (A.4). w3 = m5-m6 m7 × 100% (A.4) Where. After the crucible was fired M5 --- ash mass in grams (G); --- M6 crucible mass in grams (G); --- M7 sample mass, in grams (g). The results parallel arithmetic mean subject to measurement results (2 decimal places). Obtained in two independent measurements under repeatability conditions Absolute difference determination result is not more than 0.1%. Determination A.7 lead (Pb) of Lead content GB 5009.12 Method according determined by dry ashing a sample. A.8 Determination of total arsenic (As) of Determination of arsenic content in a method according to GB 5009.11.Appendix BAmmonium glycyrrhizinate and the components of FIGS HPLC retention time reference Ammonium glycyrrhizinate liquid chromatography shown in Figure B.1, FIG B.2. Figure B.1 Ammonium glycyrrhizinate standards (18β- ammonium glycyrrhizinate) FIG liquid chromatography Figure B.2 ammonium glycyrrhizinate FIG sample liquid chromatography ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB 1886.242-2016_English be delivered?Answer: Upon your order, we will start to translate GB 1886.242-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB 1886.242-2016_English with my colleagues?Answer: Yes. 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