GB/T 16550-2020 English PDF

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GB/T 16550-2020: Diagnostic techniques for newcastle disease
Status: Valid

GB/T 16550: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 16550-2020	299	Add to Cart	3 days	Diagnostic techniques for newcastle disease	Valid
GB/T 16550-2008	519	Add to Cart	3 days	Diagnostic techniques for new castle disease	Obsolete
GB 16550-1996	279	Add to Cart	3 days	Technical code of quarantine for newcastle disease	Obsolete

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Basic data

Standard ID: GB/T 16550-2020 (GB/T16550-2020)
Description (Translated English): Diagnostic techniques for newcastle disease
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 16,186
Date of Issue: 2020-12-14
Date of Implementation: 2020-12-14
Older Standard (superseded by this standard): GB/T 16550-2008
Quoted Standard: GB 19489; NY/T 541; NY/T 1948
Regulation (derived from): National Standard Announcement No. 28 of 2020
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration
Summary: This standard specifies the clinical diagnosis of Newcastle disease, virus isolation and identification, hemagglutination and hemagglutination inhibition tests, reverse transcription polymerase chain reaction (RT-PCR) and real-time fluorescent RT-PCR (Real-time RT-PCR) Technical requirements. This standard is applicable to the diagnosis, quarantine, detection, surveillance and epidemiological investigation of Newcastle disease.

GB/T 16550-2020: Diagnostic techniques for newcastle disease

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Newcastle disease diagnosis technology) ICS 11:220 B41 National Standards of People's Republic of China Replace GB/T 16550-2008 Newcastle disease diagnosis technology 2020-12-14 release 2020-12-14 implementation State Administration for Market Regulation Issued by the National Standardization Management Committee

Foreword

This standard was drafted in accordance with the rules given in GB/T 1:1-2009: This standard replaces GB/T 16550-2008 "Newcastle Disease Diagnostic Technology", and compared with GB/T 16550-2008, the main technical changes as follows: ---Modified the determination results of hemagglutination and hemagglutination inhibition test (see 7:3 and 7:4, 5:3 and 6:3 of the:2008 edition); --- Deleted MDT and IVPI in the virulence evaluation index (see 4:3 and 4:4 in the:2008 edition); --- Added real-time fluorescent RT-PCR detection method (see Chapter 9): Please note that certain contents of this document may involve patents: The issuing agency of this document is not responsible for identifying these patents: This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China: This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181): This standard was drafted: China Animal Health and Epidemiology Center, Yangzhou University: The main drafters of this standard: Liu Hualei, Wang Jingjing, Yu Xiaohui, Lu Yan, Wu Yantao, Hu Shunlin, Zheng Dongxia, Zhao Yunling, Liu Wenbo, Liu Xiufan and Wang Zhiliang: The previous versions of the standard replaced by this standard are as follows: ---GB 16550-1996, GB/T 16550-2008:

Introduction

Newcastledisease (ND) is a virulent strain of Newcastledisease virus (NDV) that infects poultry It caused an acute and severe infectious disease, causing huge economic losses to the world's poultry industry: The World Organization for Animal Health (OIE) will Listed as a statutory animal disease, the Ministry of Agriculture and Rural Affairs of my country has listed it as a type of animal disease: Newcastle disease virus can infect more than 240 kinds of poultry, among which domestic chickens and guinea fowls are the most susceptible: Source of infection: The Newcastle disease virus is mainly transmitted through the digestive tract and respiratory tract: Contaminated water, feed, egg trays (boxes), breeding eggs, chicken embryos and poisoned wild Live birds, insects and related personnel can all become transmission media: Newcastle disease virus belongs to Paramyxoviridae (Paramyxoviridae), Orthoavulavirus (Orthoavulavirus): The epidemic virus has only one serotype, but it can be divided into multiple genotypes: OIE stipulates that Newcastle disease is caused by a virulent strain of Newcastle disease virus in poultry Infection, therefore, for the diagnosis of Newcastle disease, in addition to identifying the Newcastle disease virus, it is also necessary to evaluate its pathogenicity: For pathogenicity assessment The estimation method can be determined by the 1-day-old SPF chicken ICPI, or molecular biology techniques, such as RT-PCR combined with sequence determination Wait: According to the partial sequence (47nt~420nt) of the F gene of Newcastle disease virus, the Newcastle disease virus can be divided into Class I and Class II: Among them, Class Ⅰ is an attenuated strain in China: Therefore, the detection method for Class Ⅰ NDV has no diagnostic significance: The diagnostic methods involved are all against the virulent strain of ClassⅡ Newcastle Disease: Newcastle disease diagnosis technology

1 Scope

This standard specifies the clinical diagnosis of Newcastle disease, virus isolation and identification, hemagglutination and hemagglutination inhibition tests, reverse transcription polymerase chain reaction (RT-PCR) and real-time fluorescent RT-PCR (Real-timeRT-PCR) technical requirements: This standard is applicable to the diagnosis, quarantine, detection, surveillance and epidemiological investigation of Newcastle disease:

2 Normative references

The following documents are indispensable for the application of this document: For dated reference documents, only the dated version applies to this article Pieces: For undated references, the latest version (including all amendments) applies to this document: GB 19489 Laboratory Biosafety General Requirements NY/T 541 Technical Specification for Collection, Storage and Transportation of Veterinary Diagnostic Samples NY/T 1948 General Rules of Biosafety Requirements for Veterinary Laboratories

3 Abbreviations

The following abbreviations apply to this document: Ct value: The number of cycles (Cyclethreshold) experienced by the amount of fluorescent signal in each reaction tube reaching the set threshold DEPC: Diethyl pyrocarbonate (Diethypyrocarbonate) HA: Hemagglutinin HI: Haemagglutinationinhibition ICPI: Intracerebralpathogenicityindex (Intracerebralpathogenicityindex) ND: Newcastledisease NDV: Newcastlediseasevirus PBS: Phosphate buffered saline (Phosphatebufferedsaline) RBC: Chicken Red Blood Cell Suspension (Redbloodcel) RNA: Ribonucleic acid (Ribonucleicacid) RT-PCR: Reverse transcription-polymerase chain reaction (Reversetranscription-polymerasechain reaction) Real-timeRT-PCR: Real-time fluorescent RT-PCR (Real-timereversetranscription-polymerasechainreac- tion) SPF: No specific pathogen (Specificpathogenfree)

4 Clinical diagnosis

4:1 Epidemiology 4:1:1 The host range is wide, chicken, turkey, quail, pigeon, goose and other poultry and wild birds are susceptible, and ducks can also be infected: 4:1:2 The source of infection is mainly infected poultry (wild bird) and poisoned poultry (wild bird), which are mainly transmitted through the digestive tract and respiratory tract: Contaminated water, feed, Egg trays (boxes), breeding eggs, chicken embryos, and poisonous wild birds, insects and related personnel can all become the main transmission media: ......

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