GB 14888.1-2010 English PDFUS$459.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB 14888.1-2010: National food safety standards -- Food additives -- New red Status: Valid GB 14888.1: Historical versions
Basic dataStandard ID: GB 14888.1-2010 (GB14888.1-2010)Description (Translated English): National food safety standards -- Food additives -- New red Sector / Industry: National Standard Classification of Chinese Standard: X42 Classification of International Standard: 67.220.20 Word Count Estimation: 20,285 Date of Issue: 2010-12-21 Date of Implementation: 2011-02-21 Older Standard (superseded by this standard): GB 14888.1-1994 Regulation (derived from): Ministry of Health Bulletin No. 19 of 2010 Issuing agency(ies): Ministry of Health of the People's Republic of China Summary: This Chinese standard applies to sulfanilic acid by diazotization with a 5 acetamido-4 naphthol -2, 7 disulfonate coupling, salting, refined food additives the new red. GB 14888.1-2010: National food safety standards -- Food additives -- New red---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.National food safety standards - food additives - new red National Food Safety Standard Red new food additive Issued on. 2010-12-21 2011-02-21 implementation National Standards of People's Republic of China People's Republic of China Ministry of Health issued ForewordThis standard replaces GB 14888.1-1994 "new food additive red." This standard compared with GB 14888.1-1994, the main changes are as follows. - Added safety tips; - New Red content ≥80% by the index revised to ≥85%; - Modify the discrimination test methods; - Colorimetric method replicates allowable difference from 2% revised to 1.0%; - An increase of chloride and sulfate indicators and detection methods, combined with the loss on drying, indicators ≤15.0%; - Cancel the quality specifications of isopropyl ether extract; - Increase the sum of the unreacted intermediate indicators and detection methods; - Added unsulfonated primary aromatic amine (aniline meter) indicators and detection methods; - Arsenic (As) is modified by chemical detection methods limit law atomic absorption spectrometry; - Cancel the heavy metals (Pb) quality specifications; - Increase the lead (Pb) indicator and detection methods. The Standard Appendix A, Appendix B and Appendix C are normative appendices, Appendix D is an informative annex. This standard replaces the standards previously issued as follows. --GB 14888.1-1994. National Food Safety Standard Red new food additive1 ScopeThis standard applies to the sulfanilic acid by diazotization with 5-acetamido-4-naphthol-2,7-disulfonate coupling, salting and refining From the new food additive red.2 Normative referencesThe standard file referenced in the application of this standard is essential. For dated references, only the edition date of the note Apply to this standard. For undated references, the latest edition (including any amendments) applies to this standard. 3 chemical name, structural formula, molecular formula, relative molecular mass 3.1 Chemical Name 7 - [(4-phenyl sulfonic acid group) azo] -1-acetylamino-8-naphthol-3,6-disulfonic acid trisodium salt 3.2 formula N a O 3 SNN OHNHCOCH 3 SO 3 N aN a O 3 S Formula 3.3 C18H12O11N3Na3S3 3.4 relative molecular mass 611.47 (according to 2007 international relative atomic mass) 4. Technical Requirements 4.1 Sensory requirements. comply with Table 1. Table 1 Sensory requirements Project requires test methods Reddish-brown color By visual assessment of natural light. State organization powders or granules 4.2 Physical indicators. to comply with Table 2. Table 2. Physical and chemical indicators Item Index Test Method The new red, w /% ≥ 85.0 Appendix A A.4 Loss on drying, chloride (based on NaCl) and sulfate (NaSO4 in dollars) of the total, w /% ≤ 15.0 Appendix A A.5 Water-insoluble, w /% ≤ 0.20 A.6 in Appendix A Deputy dye, w /% ≤ 2.0 Appendix A A.7 Unreacted intermediate sum, w /% ≤ 0.50 Appendix A A.8 Unsulfonated primary aromatic amine (aniline dollars), w /% ≤ 0.01 A.9 in Appendix A Arsenic (As)/(mg/kg) ≤ 1.0 Appendix A A.10 Lead (Pb)/(mg/kg) ≤ 10.0 Appendix A A.11Appendix A(Normative) Testing method A.1 Safety Tips Reagents The standard test methods used for toxic or corrosive, according to the relevant provisions of the operation, the operation need to be careful. If splash On the skin should immediately wash with water, severe cases should be treated immediately. When using a volatile acid, to be carried out in a fume hood. A.2 General Provisions This standard reagents and water, did not indicate when the other requirements, refer to the three water analytical reagent and GB/T 6682-2008 requirements. Standard test solution required impurity standard solution, preparations and products at the time did not indicate other provisions, according to GB/T 601, GB/T 602, GB/T 603 regulations formulated and calibration. A.3 Identification Test A.3.1 Reagents and materials A.3.1.1 sulfuric acid. A.3.1.2 ammonium acetate solution. 1.5g/L. A.3.2 Instruments and Equipment A.3.2.1 Spectrophotometer. A.3.2.2 cuvette. 10mm. A.3.3 Identification method It should meet the following conditions. A.3.3.1 Weigh about 0.1g sample (accurate to 0.001g), was dissolved in 100mL of water, red clear solution. A.3.3.2 Weigh about 0.2g sample (accurate to 0.001g), was dissolved in 20mL sulfuric acid was dark purple, take this solution 2 drops to 3 drops, 5 mL of water, shake, red. A.3.3.3 Weigh about 0.1g sample (accurate to 0.001g), was dissolved in 100mL ammonium acetate solution of this solution 1mL, plus ammonium acetate solution Equipped to 100mL, the maximum absorption wavelength of the solution was 525 nm ± 2nm. A.4 Determination new red A.4.1 Titanium trichloride titration (Arbitration Act) A.4.1.1 Method summary In acidic medium, the new red azo group is titanium trichloride reduced and decomposed into amino compounds by elimination of titanium trichloride standard titration solution Consumption, calculate the content. A.4.1.2 Reagents and materials A.4.1.2.1 trisodium citrate. A.4.1.2.2 titanium trichloride standard titration solution. c (TiCl3) = 0.1mol/L (now with the existing, method of preparation see Appendix B). A.4.1.2.3 cylinders loaded carbon dioxide. A.4.1.3 instruments and equipment A-- conical flask (a 500 mL); B-- Brown burette (50mL); C-- under glass bottle package black paper (2000mL); D-- containing 100g/L solution of ammonium carbonate and 100g/L ferrous sulfate solution container of an equivalent mixture (5000mL); E-- piston; F-- bottles; G-- washing bottle filled with water. Figure A.1 apparatus of FIG titanium trichloride titration A.4.1.4 analysis step Weigh about 0.5g sample (accurate to 0.0001g), in 500mL conical flask, 15g trisodium citrate and 200mL boiled water, After shaking dissolved, according to Figure A.1 installed equipment at surface through carbon dioxide while heating boil, with titanium trichloride standard titration solution Titration end point so that the inherent color disappeared. A.4.1.5 Calculation Results New red mass fraction 1w its value is expressed in%, according to formula (A.1) Calculated. 0) 4 /) (1000/( 1 × = m MVcw (A.1) Where. c - accurate titanium trichloride standard titration solution concentration value in units of moles per liter (mol/L); V - accurate value of the sample consumed titration of titanium trichloride standard titration solution volume in milliliters (mL); M - molar mass of new red value, in units of grams per mole (g/mol) [M (C18H12O11N3Na3S3) = 611.47]; m1 - mass value of the sample in grams (g). The results represent a decimal. The absolute difference between the parallel determination results is not more than 1.0% (mass fraction), the arithmetic mean value as a measurement result. A.4.2 Colorimetric method A.4.2.1 Method summary The samples with known content of new red standard were dissolved in water and diluted to volume with ammonium acetate solution, at the maximum absorption wavelength, respectively Measuring the absorbance, and then calculate its content. A.4.2.2 Reagents and materials A.4.2.2.1 ammonium acetate solution. 1.5g/L. A.4.2.2.2 new red standard. ≥85.0% (mass fraction, measured according to A.4.1). A.4.2.3 instruments and equipment A.4.2.3.1 spectrophotometer; A.4.2.3.2 cuvette. 10mm. A.4.2.4 preparation of new red standard sample solution Weigh about 0.25g of new red standard sample (accurate to 0.0001g), was dissolved in an appropriate amount of water, transferred to 1000mL volumetric flask, diluted with water To the mark. Draw 10mL, transferred to 500mL volumetric flask, add ammonium acetate solution was diluted to the mark, shake well and set aside. Formulating new red sample solution A.4.2.5 A.4.2.4 weighing and methods of operation with standard solution preparation. A.4.2.6 analysis step The new red standard sample solution and the new red sample solution were placed in 10mm cuvettes, with the maximum absorption wavelength measured with a spectrophotometer Given their absorbance values with ammonium acetate solution as the reference solution. A.4.2.7 Calculation Results New red mass fraction 1w its value is expressed in%, according to formula (A.2) Calculated. 1 wmA Amw × = (A.2) Where. A - absorbance values of the new red sample solution; Quality value m0-- new red standard sample in grams (g); Absorbance A0-- new red standard sample solution value; Quality m-- sample value in units of grams (g); w0-- new red standard mass fraction%. The results represent a decimal. The absolute difference between the parallel determination results is not more than 1.0% (mass fraction), the arithmetic mean value as a measurement result. A.5 Determination of loss on drying, chloride (based on NaCl) and sulfate (Na2S04 in dollars) of the total A.5.1 Determination of loss on drying A.5.1.1 analysis step Weigh about 2g sample (accurate to 0.001g), has been placed in the weighing bottle constant at 135 ℃ ± 2 ℃ constant temperature oven at 135 ℃ ± 2 ℃ Thermostatic oven drying to constant weight. A.5.1.2 Calculation Results Loss on drying mass fraction 2w and its value is expressed in%, according to formula (A.3) Calculated. 2 × - = m mmw (A.3) Where. Numerical m2-- sample before drying mass in grams (g); m3-- sample dried to a constant value of mass in grams (g). The results represent a decimal. The absolute difference between the parallel determination results is not more than 0.2% (mass fraction), the arithmetic mean value as a measurement result. A.5.2 chloride (as NaCl) Determination A.5.2.1 Reagents and materials A.5.2.1.1 nitrobenzene. A.5.2.1.2 activated carbon; 767 needle. A.5.2.1.3 nitric acid solution. 11. A.5.2.1.4 silver nitrate solution. c (AgNO3) = 0.1mol/L. A.5.2.1.5 ammonium ferric sulfate solution Preparation method. Weigh about 14g of ammonium ferric sulfate, dissolved in 100mL of water, filter, add 10mL of nitric acid, stored in a brown bottle; A.5.2.1.6 ammonium thiocyanate standard titration solution. c (NH4CNS) = 0.1mol/L. A.5.2.2 preparation of the sample solution Weigh about 2g sample (accurate to 0.001g), was dissolved in 150mL of water, add about 15g of activated carbon, a moderate boil 2 min ~ 3min, added 1mL solution of nitric acid, constantly rocking evenly placed 30min (during shaking from time to time). Filtered through a dry filter paper. Such as colored filtrate is combined with 5g Activated carbon, occasionally shaking place 1h, then dried filter paper (such as color still replace the activated carbon Repeat until the filtrate colorless). Each time 10mL washed activated charcoal three times, combined filtrate move 200mL volumetric flask, add water to the mark. For chloride and sulfate content Determination. A.5.2.3 analysis step Pipette 50mL sample solution, placed in 500mL conical flask, add 2mL nitric acid solution and 10mL silver nitrate solution (chloride content for a long time To add more) and 5mL nitrobenzene, shake vigorously to condense silver chloride was added 1mL solution of ammonium ferric sulfate, ammonium thiocyanate standard titration solution Titrate the excess silver nitrate to the end and keep 1min, at the same time in the same way to make a blank test. A.5.2.4 Calculation Results Chloride (as NaCl) mass fraction 3w and its value is expressed in%, according to formula (A.4) Calculated. ) 200/50 ( ] 1000 /) [( 3 × - = m MVVcw (A.4) Where. c1 - accurate ammonium thiocyanate standard titration solution concentration value in units of moles per liter (mol/L); V1 - accurate value of consumption of titration blank solution of ammonium thiocyanate standard titration solution volume in milliliters (mL); V0 - accurate value of the sample solution was titrated with ammonium thiocyanate standard titration solution consumed volume in milliliters (mL); M1 - the value of the molar mass of sodium chloride, in units of grams per mole (g/mol) [M1 (NaCl) = 58.4]; Mass values m4-- sample in grams (g). The results represent a decimal. Parallel determination results of absolute difference is not more than 0.3% (mass fraction), the arithmetic mean value as a measurement result. A.5.3 Sulfate (Na2S04 meter) measurement A.5.3.1 Reagents and materials A.5.3.1.1 sodium hydroxide solution. 2g/L. A.5.3.1.2 hydrochloric acid solution. 11999. A.5.3.1.3 barium chloride standard titration solution. c (1/2BaCl2) = 0.l mol/L (preparation see Appendix C). A.5.3.1.4 phenolphthalein indicator solution. 10g/L. A.5.3.1.5 Rose sodium indicator solution. Weigh 0.lg red roses, sodium dissolved in 10mL of water (using now). A.5.3.2 analysis step Draw 25mL sample solution A.5.2.2, placed in 250mL conical flask, add 1 drop of phenolphthalein indicator solution, a solution of sodium hydroxide solution was pink, Then a solution of hydrochloric acid solution to the pink color disappeared, shake, shaking constantly dissolved barium chloride standard titration solution titration, acid red roses Sodium indicator solution for outward indicator solution, and the reaction liquid indicator solution on filter paper presents the intersection of rose red spots and kept 2min does not fade as the end point. At the same time in the same manner as a blank test. A.5.3.3 Calculation Results Sulfate (Na2SO4 meter) mass fraction 4w and its value is expressed in%, according to formula (A.5) Calculated. ) 200/25 ( ) 2 /] (1000 /) [( 4 × - = m MVVcw (A.5) Where. c2 - accurate barium chloride standard titration solution concentration value in units of moles per liter (mol/L); The exact value of the sample solution V2-- titration consumption of barium chloride standard titration solution volume in milliliters (mL); Accurate value of V3-- titrate blank solution consumed barium chloride standard titration solution volume in milliliters (mL); M2 - the value of the molar mass of sodium sulfate, units of grams per mole (g/mol) [M2 (Na2SO4) = 142.04]; m4 - mass of the sample value in units of grams (g). The results represent a decimal. The absolute difference between the parallel determination results is not more than 0.2% (mass fraction), the arithmetic mean value as a measurement result. Results A.5.4 Loss on drying, chloride (based on NaCl) and sulfate (Na2SO4 in dollars) the total amount of calculation Total loss on drying and chloride (based on NaCl) and sulfate (Na2SO4 to count) to the mass fraction 5w and its value is expressed in%, according to public Formula (A.6) Calculated. 4325 wwww = (A.6) Where. 2w - drying loss mass fraction%; 3w - chloride (as NaCl) mass fraction%; 4w - Sulfate (Na2SO4 meter) mass fraction%. The results represent a decimal. A.6 Determination of insoluble matter A.6.1 Instruments and Equipment A.6.1.1 sand core glass crucible. G4, a pore size of 5μm ~ 15μm. A.6.1.2 oven thermostat. A.6.2 Analysis step Weigh about 3g sample (accurate to 0.001g), placed in 500mL beaker, add 50 ℃ ~ 60 ℃ hot water 250mL, dissolved with It has been baked at 135 ℃ ± 2 ℃ to constant weight of G4 sintered glass crucible filtration, and thoroughly washed with hot water to wash colorless liquid at 135 ℃ ± 2 ℃ constant Constant temperature oven to bake. A.6.3 Calculation Results Water insoluble mass fraction 6w and its value is expressed in%, according to formula (A.7) calculated as follows. 6 × = m mw (A.7) Where. m6 - Numerical dried water insoluble mass in grams (g); m5 - the value of the sample mass, in grams (g). The results represent two decimal. The absolute difference between parallel determination results is not more than 0.05% (mass fraction), the arithmetic mean value as a measurement result. Determination A.7 deputy dye A.7.1 Method summary The components are separated by paper chromatography, eluted and quantified by spectrophotometry. A.7.2 Reagents and materials A.7.2.1 ethanol. A.7.2.2 n-butanol. Acetone solution A.7.2.3. 1 1. A.7.2.4 ammonia solution. 496. A.7.2.5 sodium bicarbonate solution. 4g/L. A.7.3 Instruments and Equipment A.7.3.1 Spectrophotometer. A.7.3.2 chromatography filter paper. No. 1 in speed, 150mm × 250mm. A.7.3.3 chromatography tank. φ240mm × 300mm. A.7.3.4 micro injector. 100μL. A.7.3.5 Nessler colorimetric tube. 50mL glass grinding mouth stopper. A.7.3.6 glass frit funnel. G3, pore size of 15μm ~ 40μm. A.7.3.7 50mm cuvette. A.7.3.8 10mm cuvette. A.7.4 Analysis step A.7.4.1 paper chromatographic conditions A.7.4.1.1 developing solvent. n-butanol ethanol solution of aqueous ammonia = 623. A.7.4.1.2 Temperature. 20 ℃ ~ 25 ℃. A.7.4.2 preparation of the sample solution Weigh 1g sample (accurate to 0.001g), placed in a beaker, adding the right amount of water dissolved and transferred to 100mL volumetric flask, dilute to Scale, shake up the concentration of the sample solution is 1%. A.7.4.3 wash out the sample preparation liquid With micro-injector draw 100μL sample solution evenly note on the bottom edge of the filter paper from a baseline of 25mm, a straight line, so that On filter paper width is not more than 5mm, length 130mm, with a hair dryer. The filter paper containing the preparation of good chromatography developing solvent Expand the cylinder, the bottom edge of the filter paper dipped under the agent level l0mm, to be solvent front line rose to 150mm or until the dye separation satisfaction deputy until. Remove the filter paper chromatography, with cold dry. Blank filter paper under the same conditions to expand the blank paper and the above steps should be expanded with the adjacent portion of the filter paper on the same piece of filter paper cut take. Deputy dye paper chromatography is shown in Figure A.2. Baseline Vice-dye (2) Main dye Deputy dye (1) 130mm 150mm 250mm 25mm Figure A.2 deputy dye paper chromatography schematic The respective deputy dye expanded and made on a blank paper to each subsidiary colors corresponding parts of the paper in the same size cut, and cut Into thin strips about 5mm × 15mm, and were placed in 50mL of Nessler colorimetric tube, accurately added 5mL acetone, shaking 3min ~ 5min , Then sodium bicarbonate solution was accurately added to 20mL, shake well, and then were naturally filtered in a sintered glass funnel, the filtrate should be clarified, Without suspension. Add water to the mark. Respectively each subsidiary colors and blank eluate. In the respective maximum absorption wavelength of the dye deputy, with 50mm Cuvette, each deputy dye sample eluate respective measured absorbance values on a spectrophotometer. When the absorbance was measured on a spectrophotometer, with a mixture of sodium bicarbonate solution 5mL and 20mL acetone solution as reference solution. A.7.4.4 preparation of standard solution Draw a sample solution 2mL 1% moved into 100mL volumetric flask, dilute to the mark, shake, and the solution as the standard solution. A.7.4.5 Preparation of standard eluate With micro-injector to draw standard solution 100μL, uniform injection site on the bottom edge of the filter paper from a baseline of 25mm, with a hair dryer. The filter paper containing previously prepared well eluent chromatography tank to expand, to be solvent front line up 40mm, remove with cold dry, cut All dyes partially deployed, according to the method of operation A.7.4.3, obtained eluate standards. 10mm cuvette with maximum absorption wave Strengths measured absorbance values. Meanwhile blank filter paper under the same conditions to start operating in the same mann......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB 14888.1-2010_English be delivered?Answer: Upon your order, we will start to translate GB 14888.1-2010_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB 14888.1-2010_English with my colleagues?Answer: Yes. The purchased PDF of GB 14888.1-2010_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.Question 5: Should I purchase the latest version GB 14888.1-2010?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB 14888.1-2010 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. |
