GB 14753-2010 English PDFUS$229.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB 14753-2010: National food safety standards -- Food additives -- Vitamin B6 (hydrochloric acid, pyridoxine alcohol) Status: Valid GB 14753: Historical versions
Basic dataStandard ID: GB 14753-2010 (GB14753-2010)Description (Translated English): National food safety standards -- Food additives -- Vitamin B6 (hydrochloric acid, pyridoxine alcohol) Sector / Industry: National Standard Classification of Chinese Standard: X42 Classification of International Standard: 67.220.20 Word Count Estimation: 10,110 Date of Issue: 2010-12-21 Date of Implementation: 2011-02-21 Older Standard (superseded by this standard): GB 14753-1993 Regulation (derived from): Ministry of Health Bulletin No. 19 of 2010 Issuing agency(ies): Ministry of Health of the People's Republic of China Summary: This Chinese standard applies to chemical synthesis of food additives vitamin B6. GB 14753-2010: National food safety standards -- Food additives -- Vitamin B6 (hydrochloric acid, pyridoxine alcohol)---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. National food safety standards - food additives - vitamin B6 (hydrochloric acid, pyridoxine alcohol) National Food Safety Standard Food additives Vitamin B6 (pyridoxine hydrochloride) Issued on. 2010-12-21 2011-02-21 implementation National Standards of People's Republic of China People's Republic of China Ministry of Health issued ForewordThis standard replaces GB 14753-1993 "Food additive vitamin B6". This standard compared with GB 14753-1993, the main changes are as follows. - Added Infrared Spectroscopy; - Vitamin B6 ≥98.0% by the index revised to 98.0% - 100.5%; - Modify the method for the determination of heavy metals, indicators from the ≤0.003% revised to ≤10mg/kg; - Added arsenic indicators and measurement methods. The Standard Annexes A and B are normative appendix. This standard replaces the standards previously issued as follows. --GB 14753-1993 National Food Safety Standard Food additives Vitamin B6 (pyridoxine hydrochloride)1 ScopeThis standard applies to chemical synthesis of food additives vitamin B6.2 Normative referencesThe standard file referenced in the application of this standard is essential. For cited documents with dates, only the date of Version applies to this standard. For undated references, the latest edition (including any amendments) applies to this standard. 3 chemical name, molecular formula, molecular mass and structural formula 3.1 Chemical Name 6-methyl-5-hydroxy-3,4-dimethanol pyridine hydrochloride Formula 3.2 C8H11NO3 · HCl 3.3 formula 3.4 relative molecular mass 205.64 (according to 2007 international relative atomic mass) 4. Technical Requirements 4.1 Sensory requirements. comply with the requirements of Table 1. Table 1 Sensory requirements Project requires test methods White or white color proper amount of sample is placed in a clean, dry test tube, in natural light The line, observe the color and texture, smell the smell. Odour odorless Organization state crystal or crystalline powder 4.2 Physical indicators. in accordance with Table 2. Table 2. Physical and chemical indicators Item Index Test Method Vitamin B6 (C8H11NO3 · HCl, dry basis), w /% 98.0 ~ 100.5 Appendix A A.4 Loss on drying, w /% ≤ 0.5 A.5 in Appendix A Residue on ignition, w /% ≤ 0.1 A.6 in Appendix A pH (100g/L solution) 2.4 ~ 3.0 GB/T 9724 Arsenic (As)/(mg/kg) ≤ 2 Appendix A A.7 Heavy metals (Pb)/(mg/kg) ≤ 10 Appendix A A.8Appendix A(Normative) Testing method A.1 Safety Tips Reagents used in this test method has toxic or corrosive, according to the relevant provisions of the operation, should be used with caution. If the Splashed on the skin should immediately wash with water, severe cases should be treated immediately. When using a volatile acid, to be carried out in a fume hood. A.2 General Provisions The reagents used in this standard, unless otherwise noted, only in the analysis confirmed analytically pure reagents and GB/T 6682-2008 in Regulation Given three water. Test Methods standard titration solution, when measured impurity standard solution, preparations and products, with no other requirements noted, According to GB/T 601, GB/T 602, the provisions of the preparation of GB/T 603's. A.3 Identification Test A.3.1 Reagents and materials A.3.1.1 sodium acetate solution.200g/L. A.3.1.2 boric acid. 40g/L. A.3.1.3 chloro-imino-2,6-dichloro-quinone solution. 5g/L ethanol solution. A.3.1.4 manganese dioxide. A.3.1.5 sulfuric acid. A.3.1.6 starch - iodide test paper. A.3.2 Analysis step A.3.2.1 color reaction Take about 10 mg laboratory samples, after adding 100 mL of water dissolved from each 1 mL, respectively, set A, B two tubes, add 2 mL each Sodium acetate solution, A tube was added 1 mL of water, the tube was added 1 mL of acetic acid solution, mix, add 1 mL of each rapid-chloro-2,6-imino Dichloro-quinone solution A tube was blue, disappeared after a few minutes, and converted to red; tube B was not blue. A.3.2.2 infrared absorption spectrum identification Using potassium bromide tablet method, infrared spectroscopy laboratory samples should be consistent with the control of the map (see map control Appendix B). A.3.2.3 Identification chloride 0.5g of laboratory samples, test tube, add 0.5g of manganese dioxide, mixing, wet sulfuric acid, slowly heated, namely chlorine generation Gas, moistened with water to make starch - iodide test paper was blue. A.4 Determination of vitamin B6 A.4.1 Method summary Crystal violet as indicator, titrate with perchloric acid standard solution sample, according to the amount of standard titration with perchloric acid solution, in order to calculate Vitamin B6 content C8H11NO3 · HCl meter. A.4.2 Reagents and materials A.4.2.1 glacial acetic acid. A.4.2.2 mercuric acetate solution. Take 5 g mercuric acetate, and research fine, plus warm glacial acetic acid to dissolve into 100 mL. A.4.2.3 crystal violet indicator solution. 5 g/L of glacial acetic acid. A.4.2.4 perchloric acid standard titration solution. c (HClO4) = 0.1 mol/L. A.4.3 Analysis step Weigh 0.15 g laboratory samples, accurate to 0.000 2 g, after 20 mL glacial acetic acid and 5 mL of mercuric acetate solution was warmed to dissolve Canada, Cool to room temperature, add 1 drop of crystal violet indicator solution, titration with perchloric acid standard solution titration until the solution was blue-green, and titration With blank test correction. A.4.4 Calculation Results Vitamin B6 (in C8H11NO3 · HCl meter) mass fraction w1, expressed in%, according to formula (A.1) Calculated. () 100 VV c Mw - × × = ××% (A.1) Where. V - standard test solution consumed perchloric acid titration solution (A.4.2.4) the value of the volume, in milliliters (mL); V0 - blank test consumed perchloric acid standard titration solution (A.4.2.4) the value of the volume, in milliliters (mL); c - accurate perchloric acid standard titration solution concentration value in units of moles per liter (mol/L); m - Numerical sample mass (dry product) in grams (g); M - Numerical Vitamin B6 (C8H11NO3 · HCl) molar mass of the units of grams per mole (g/mol) (M = 205.64). Take two parallel determination results of the arithmetic average of the measurement results, the results of two parallel determination of the absolute difference is not more than 0.5%. A.5 Determination of loss on drying A.5.1 Analysis step Weigh 1.0 g laboratory samples, accurate to 0.000 2 g, according to GB/T 6284 Method Determination. A.5.2 Calculation Results Loss on drying mass fraction w2, expressed in%, according to equation (A.2) Calculated. twenty one 2 100% m mw - = × (A.2) Where. m2 - weighing bottle before drying and the total mass of the sample in grams (g); m1 - After drying the samples and weighing bottle total mass in grams (g); m - mass of the sample in grams (g). Take the arithmetic mean of the parallel determination results of the measurement results, the results of two parallel determination of the absolute difference is not more than 0.05%. A.6 Residue on ignition A.6.1 Reagents and materials sulfuric acid. A.6.2 Analysis step Weigh about 1.0 g laboratory samples, accurate to 0.000 2 g, ignition has been set to a constant weight of the crucible, slowly burn to completely carbonized, Cool to room temperature; adding 0.5 mL ~ 1 mL of sulfuric acid so moist, low-temperature heating to sulfuric acid vapor divisible after burning at 750 ℃ ± 50 ℃ enable End Full ashing, moved to a dryer, cooled to room temperature, and precisely weighed after set, then at 750 ℃ ± 50 ℃ ignition to constant weight. A.6.3 Calculation Results Burning residue mass fraction w3, expressed in%, according to formula (A.3) Calculated. m mw - = ×% (A.3) Where. m4 - the value of the total mass of the crucible and residue, in grams (g); m3 - the value crucible mass in grams (g); m - mass of sample values in grams (g). Take the arithmetic mean of the parallel determination results of the measurement results, the results of two parallel determination of the absolute difference is not more than 0.02%. A.7 Determination of Arsenic Weigh 1.0 g ± 0.01 g laboratory samples, add 10 mL of water was dissolved as a sample solution; amount of 2 mL ± 0.02 mL (arsenic 2.0 μg) Arsenic (As) standard solution preparation limits. Other conducted in accordance with GB/T 5009.76-2003 Gutzeit method. A.8 Determination of Heavy Metals A.8.1 Reagents and materials A.8.1.1 nitrate. A.8.1.2 glycerol. A.8.1.3 ammonium acetate. A.8.1.4 lead nitrate. A.8.1.5 thioacetamide. A.8.1.6 hydrochloric acid solution. c (HCl) = 2 mol/L. A.8.1.7 ammonia solution. c (NH3 · H2O) = 5 mol/L. A.8.1.8 sodium hydroxide solution. c (NaOH) = 1 mol/L. A.8.1.9 hydrochloric acid solution. c (HCl) = 7 mol/L. A.8.1.10 acetate buffer (pH3.5). Weigh approximately 25 g of ammonium acetate, accurate to 0.01 g, add 25 mL of water dissolved, add 7 mol L hydrochloric acid/38 mL, with 2 mol/L Or a hydrochloric acid solution 5 mol/L aqueous ammonia solution, the pH adjusted to accurately 3.5 of 5 (pH meter), diluted with water to 100 mL. A.8.1.11 thioacetamide test solution. It weighs about 4 g thioacetamide, to the nearest 0.01 g, add water to dissolve into a 100 mL, home refrigerator. Take 5.0 mL prior to use Mixture (by a 1 mol/L 15 mL of sodium hydroxide solution, 5.0 mL of water and 20 mL glycerol), plus the aforementioned 1.0 mL of thioacetamide solution Liquid, water bath heated on 20s, cooled and used immediately. A.8.1.12 lead standard solution. Weigh about 0.160 g of lead nitrate, accurate to 0.000 2g, placed in 1000 mL volumetric flask, add 5 mL of nitric acid and 50 mL of water to dissolve After diluted with water to the mark, shake, as the stock solution. Before use, pipette 10 mL ± 0.02 mL stock solution into 100 mL capacity Volumetric flask, diluted with water to the mark, shake, that was (per 1mL equivalent to 10 μg of Pb). Configuration and glassware are used in storage It may not lead. A.8.2 Analysis step Press the "People's Republic of China Pharmacopoeia" 2005 edition Appendix Ⅷ H method first heavy metal inspection method. Here's how. Take 25 mL Nessler colorimetric tube two, A tube was charged with 2 mL ± 0.2 mL (leaded 20.0 μg) of lead (Pb) and 2 mL standard solution After acetate buffer, diluted with water to 25 mL; 2.0 g Weigh other laboratory samples, accurate to 0.01 g, placed Nessler colorimetric tube B Tube, add 20 mL of water dissolved, add ammonia solution to the litmus paper in case of neutral reaction, add 2 mL acetate buffer (pH3.5), Diluted with water to 25 mL, if the solution is colored, can be dropping a small amount of a dilute solution or other interference-free caramel colored solution A tube To make it consistent with the B pipe; and then were added to the two B thioacetamide test solution in each of 2 mL, shake, place 2min, on the same set of paper, Top-down perspective, B show the color of the tube A tube, not deeper.Appendix B(Normative) Vitamin B6 IR Note. Quoted from "Drug IR set," Volume I (1995 -) Figure B.1 vitamin B6 IR ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB 14753-2010_English be delivered?Answer: Upon your order, we will start to translate GB 14753-2010_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB 14753-2010_English with my colleagues?Answer: Yes. The purchased PDF of GB 14753-2010_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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