GA/T 766-2020 English PDF

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GA/T 766-2020: Methods for detection of PSA in human semen - Rapid test strip
Status: Valid

GA/T 766: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GA/T 766-2020	139	Add to Cart	3 days	Methods for detection of PSA in human semen - Rapid test strip	Valid
GA 766-2008	199	Add to Cart	3 days	Detection method of human semen PSA--Rapid test strip	Obsolete

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Basic data

Standard ID: GA/T 766-2020 (GA/T766-2020)
Description (Translated English): Methods for detection of PSA in human semen - Rapid test strip
Sector / Industry: Public Security (Police) Industry Standard (Recommended)
Classification of Chinese Standard: A92
Classification of International Standard: 13.310
Word Count Estimation: 6,611
Date of Issue: 2020-03-12
Date of Implementation: 2020-05-01
Older Standard (superseded by this standard): GA 766-2008
Issuing agency(ies): Ministry of Public Security

GA/T 766-2020: Methods for detection of PSA in human semen - Rapid test strip

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Human semen PSA detection gold standard reagent strip method) ICS 13.310 A92 People's Republic of China Public Safety Industry Standard Replace GA 766-2008 Human semen PSA test Gold-labeled reagent strip method 2020-03-12 released 2020-05-01 Implementation Issued by the Ministry of Public Security of the People's Republic of China

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces GA 766-2008 "Human Semen PSA Detection Gold Standard Reagent Strip Method". Compared with GA 766-2008, the main technical changes are as follows. ---Revised as a recommended standard (see cover, cover of.2008 edition); ---Added the requirements for pure water (see 4.1). This standard was proposed and managed by the Forensic Examination Subcommittee of the National Criminal Technology Standardization Technical Committee (SAC/TC179/SC6). This standard was drafted by. Material Evidence Appraisal Center of the Ministry of Public Security, Heilongjiang Provincial Public Security Department. The main drafters of this standard. Wen Peizhong, Bai Xue, Meng Qingzhen, Euro, Zhang Da'an, Liu Yihui, Li Xiaoping, Zhu Aimin, Li Peng, Liu Xudan, Chang Caiqin and Zhang Yinglan. The previous versions of the standard replaced by this standard are as follows. ---GA 766-2008. Human semen PSA test Gold-labeled reagent strip method

1 Scope

This standard stipulates the test principles for the rapid test of human prostate specific antigen (PSA) through gold-labeled reagent strips in forensic physical evidence testing Matters needing attention in material preparation, operation method, result judgment and inspection. This standard applies to human semen (spots) PSA related to sexual crimes in forensic science examinations, including vagina, oral cavity, anus, and skin wipes And toilet paper, clothing and other biological samples that may adhere to human semen (spots).

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this document. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682-2008 Analysis laboratory water specifications and test methods GA 477 Human Prostate Specific Antigen (PSA) Gold Standard Test Strip

3 Principle

The basic principle of the gold-labeled reagent strip method is the gold-labeled double antibody sandwich method. The gold-labeled reagent strip is divided into three zones, namely the sample addition zone, the reaction zone and the adsorption zone. Gold-labeled monoclonal anti-human PSA antibody adsorbed on the sample area After the body reacts with the human sperm spot soaking solution (containing human PSA), a gold-labeled antigen-antibody complex is formed, and diffuses to the adsorption area of the reagent strip. When the substance diffuses to the reaction zone, it binds to another monoclonal anti-human PSA antibody adsorbed in this zone (linear), and the antigen-antibody complex adsorbs here, A purple-red line (positive reaction line) is formed. Excess complexes continue to diffuse to the adsorption zone, and the other antibody adsorbed in the reaction zone (usually goat antibody) Mouse IgG) combined to form a purple-red line (quality control line). If the soaking solution does not contain human PSA, the antigen-antibody complex cannot be formed in the sample application area, and the gold-labeled anti-human diffusion to the reaction area PSA antibody cannot bind to another monoclonal anti-human PSA antibody adsorbed in this area, and there is no purple-red linear band formed. when it Continue to diffuse to the adsorption zone and combine with another goat anti-mouse IgG adsorbed in the reaction zone to form a purple-red line (quality control line).

4 Reagents and equipment

4.1 Reagents The reagents include. a) Human PSA gold standard test kit (in line with the requirements of GA 477); b) Pure water (conforms to the tertiary water requirements specified in GB/T 6682-2008). 4.2 Equipment Equipment includes. a) ophthalmic scissors ......

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