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GA 655-2006 English PDF

Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GA 655-2006219 Add to Cart 3 days Detection method for ABO blood type in human hairs--Elution test Obsolete

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Basic data

Standard ID: GA 655-2006 (GA655-2006)
Description (Translated English): Detection method for ABO blood type in human hairs--Elution test
Sector / Industry: Public Security (Police) Industry Standard
Classification of Chinese Standard: A92
Classification of International Standard: 13.310
Word Count Estimation: 5,529
Date of Issue: 2006-11-03
Date of Implementation: 2007-03-01
Quoted Standard: GA 471-2004
Issuing agency(ies): Ministry of Public Security
Summary: This standard prescribes the material preparation, operation method and result judgment of ABO blood type test of human hair in forensic biological material examination. This standard is applicable to the forensic examination of human hair ABO blood type.

GA 655-2006: Detection method for ABO blood type in human hairs--Elution test

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection method for ABO blood type in human hairs--Elution test ICS 13.310 A92 People's Republic of China Public Safety Industry Standard Human hair ABO blood group detection dissociation method Released on November 11,.2006 2007-03-01 implementation Ministry of Public Security

Foreword

4.1犪 of this standard is mandatory and the rest are recommended. Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Criminal Technology Standardization Committee. This standard was drafted. Ministry of Public Security Material Certification Center, Heilongjiang Provincial Public Security Department. Drafters of this standard. Wen Peizhong, Zhang Daan, Liu Jun, Li Xiaoping, Zhu Aimin, Li Wei, Liu Xudan, Chang Caiqin, Zhang Yinglan, Li Zhaolong, etc. Human hair ABO blood group detection dissociation method

1 Scope

This standard stipulates the material preparation, operation method and result judgment of human hair ABO blood group test in the physical examination of the doctor. This standard applies to the forensic science of human hair ABO blood type.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GA 471-2004 anti-A, anti-B serum reagent

3 Principle

The dissociation method is an antigen-antibody complex formed by the reaction of an antigen and an antibody, and the antigen-antibody complex can be released under certain conditions. Antibodies are designed to maintain the original properties of antigens and antibodies. The ABO blood group substance in the hair sample is present in the medulla In the case where an anti-A antibody or an anti-B antibody is present, an antigen-antibody reaction occurs to form an antigen-antibody complex. When at 50 ° C ~ 56 ° C Under the condition, the antigen-antibody complex dissociates and the antibody is released into the liquid. When the temperature is lowered to about 37 ° C, the free antibody and phase The agglutination reaction of the red blood cell antigen should be indicated, and agglomerates can be observed under the microscope. According to the corresponding indication of red blood cell agglutination The ABO blood type can be judged according to the situation.

4 Reagents and equipment

4.1 Reagents a) Standard anti-A antibody, standard anti-B antibody reagent. Meet the requirements of GA 471-2004; b) Type A and Type B indicate red blood cell suspension (0.2%) (see Appendix A); c) absolute ethanol; d) anhydrous ether; e) washing liquid; f) saline. 4.2 Equipment a) bench top centrifuge; b) optical microscope; c) a vacuum suction device; d) an incubator; e) refrigerator; f) the applicator and the applicator tip; g) ophthalmic scissors, ophthalmology; h) a crimping machine or other alternative equipment or equipment; i) concave glass plate; j) slides, coverslips, slide holders, humidors, capillaries; k) Marker, filter paper, cotton wool.
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