GB/T 5522-2008 English PDF

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GB/T 5522-2008: Inspection of grains and oils -- Determination of hydrogen peroxidase activity of grain and oilseeds
Status: Valid

GB/T 5522: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 5522-2008	139	Add to Cart	3 days	Inspection of grains and oils -- Determination of hydrogen peroxidase activity of grain and oilseeds	Valid
GB/T 5522-1985	199	Add to Cart	2 days	Inspection of grain and oilseeds--Methods for determination of hydrogen peroxides activity	Obsolete

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Basic data

Standard ID: GB/T 5522-2008 (GB/T5522-2008)
Description (Translated English): Inspection of grains and oils -- Determination of hydrogen peroxidase activity of grain and oilseeds
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.040
Word Count Estimation: 6,665
Date of Issue: 2008-11-04
Date of Implementation: 2009-01-20
Older Standard (superseded by this standard): GB/T 5522-1985
Regulation (derived from): National Standard Approval Announcement 2008 No.18 (Total No.131)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the grain, fuel in catalase activity measurement terminology and definitions, principle, reagents and materials, steps, , sample preparation, instruments and appliances, and repeatability of the results of the calculation. This standard applies to food, determination of oil in catalase activity of.

GB/T 5522-2008: Inspection of grains and oils -- Determination of hydrogen peroxidase activity of grain and oilseeds

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of grains and oils. Determination of hydrogen peroxidase activity of grain and oilseeds ICS 67.040 X04 National Standards of People's Republic of China Replacing GB/T 5522-1985 Inspection of grain Determination of grain and oilseeds of catalase activity of Posted 2008-11-04 2009-01-20 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard replaces GB/T 5522-1985 "grain and oilseeds testing catalase activity assay." The main changes compared to 5522-1985 standard and GB/T as follows. --- Increasing the terms and definitions; --- Modify the concentration of the concentration of potassium permanganate standard solution and hydrogen peroxide solution; --- Unified reaction temperature and time; --- Increase the reproducibility of test results requirements. The standard proposed by the National Food Authority. This standard by the National Standardization Technical Committee OILS. This standard was drafted. Anhui Grain and Oil Product Quality Supervision and Inspection Station, Henan University of Technology, the National Food Authority Chengdu Grain Storage science Institute, Tianjin Grain and Oil inspection station. The main drafters of this standard. a quarter of cis, MINORITIES, Zhang Hao, Li Xueqin, Cheng Jianhua, Zhao Shuyuan, peach Wang, Wu Yun, Wang Jing. This standard replaces the standards previously issued as follows. --- GB/T 5522-1985. Inspection of grain Determination of grain and oilseeds of catalase activity of

1 Scope

This standard specifies the grain and oilseeds in catalase activity Determination of terms and definitions, principles, reagents and materials, apparatus and appliances, Sample preparation steps, the results of calculation and repeatability. This standard applies to food, measured in oil catalase activity of.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Preparation of GB/T 601 chemical reagent standard titration solution GB 5491 grain and oilseeds testing for sampling, and sample method GB/T 5497 grain and oilseeds - Determination of water test GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987. MOD)

3 Terms and Definitions

The following terms and definitions apply to this standard. 3.1 Under specified conditions, a certain amount of amount of hydrogen peroxide in a sample and catalase and hydrogen peroxide consumption, per gram of sample (dry basis) Hydrogen peroxide consumed expressed in milligrams. Principle 4 Under pH7.7 conditions, catalase extracted from the sample, adding a certain amount in the extract hydrogen peroxide, hydrogen peroxide in the past Catalase decomposes action, and then potassium permanganate titration of excess hydrogen peroxide. According to the consumption of potassium permanganate solution is calculated again Samples catalase activity.

5 Reagents and materials

The following reagents were analytical grade reagents. 5.1 Water. in line with GB/T 6682 laboratory water three requirements. 5.2 2% hydrogen peroxide solution. Take 30% hydrogen peroxide solution 2mL, diluted with water to 30mL. 5.3 10% sulfuric acid solution. 10mL water and 90mL of concentrated sulfuric acid were added. 5.4 potassium permanganate standard solution. Ba (1/5KMnO4) = 0.2mol/L, according to GB/T 601 formulation and calibration. 5.5 pH7.7 phosphate buffer Soren Johnson. A solution. Weigh-hydrogen phosphate, disodium 11.876g, dissolved in 1L of water. B solution. Weigh potassium dihydrogen phosphate 9.078g, dissolved in 1L of water. When you use, A solution, B solution mixed by 9.1. ......

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