GB/T 5521-2008 English PDF

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GB/T 5521-2008: Inspection of grain and oils -- Determination of alpha-amylase activity in cereal and cereal products -- Colorimetric method
Status: Valid

GB/T 5521: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 5521-2008	209	Add to Cart	3 days	Inspection of grain and oils -- Determination of alpha-amylase activity in cereal and cereal products -- Colorimetric method	Valid
GB/T 5521-1989	199	Add to Cart	2 days	Method for determination of alpha-amylase activity in cereal and cereal products - Colorimetric method	Obsolete

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Basic data

Standard ID: GB/T 5521-2008 (GB/T5521-2008)
Description (Translated English): Inspection of grain and oils -- Determination of alpha-amylase activity in cereal and cereal products -- Colorimetric method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B20
Classification of International Standard: 67.040
Word Count Estimation: 9,948
Date of Issue: 2008-11-04
Date of Implementation: 2009-01-01
Older Standard (superseded by this standard): GB/T 5521-1989
Regulation (derived from): National Standard Approval Announcement 2008 No.17 (Total No.130)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the colorimetric determination of grain and products of ��- amylase activity of terms and definitions, principle, reagents and materials, instrument, steps, calculated results, precision and test reports. This standard applies to the determination of cereals and cereal products ��- amylase activity, can also be used to determine from fungal and bacterial ��- amylase activity of powder.

GB/T 5521-2008: Inspection of grain and oils -- Determination of alpha-amylase activity in cereal and cereal products -- Colorimetric method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of grain and oils. Determination of alpha-amylase activity in cereal and cereal products. Colorimetric method ICS 67.040 B20 National Standards of People's Republic of China Replacing GB/T 5521-1989 Inspection of grain and grain products α- amylase Colorimetric Determination of Activity Posted 2008-11-04 2009-01-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard is GB/T 5521-1989 "cereals and cereal products α- amylase activity measuring method" of amendments. This standard replaces GB/T 5521-1989. This standard compared with GB/T 5521-1989 The main changes are as follows. --- Increasing the normative references; --- Terminology and definitions have been modified; --- Increased test reporting requirements; --- Will be calculated by the body adjusted to the appendix for example, as an information appendix. Appendix A of this standard is an informative annex. The standard proposed by the National Food Authority. This standard by the National Standardization Technical Committee OILS. This standard was drafted. Nanjing University of Finance and Economics. The main drafters of this standard. OILS, Wang Suya, Yuan Jian, Yang Huiping. This standard replaces the standards previously issued as follows. --- GB/T 5521-1985, GB/T 5521-1989. Inspection of grain and grain products α- amylase Colorimetric Determination of Activity

1 Scope

This standard specifies the colorimetry and cereal products α- amylase activity terms and definitions, principles, reagents and materials, equipment, operation Procedures, calculation results, precision and test reports. This standard applies to the determination of cereals and cereal products α- amylase activity can also be used to determine derived from fungi and bacteria α- amylase powder Activity.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to this part of the research agreement Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

3 Terms and Definitions

The following terms and definitions apply to this standard. 3.1 Β- amylase can be completely degraded starch product. 3.2 Samples extracted from 1g enzyme 1L solvent, under specified conditions, the degradation of β- limit dextrin substrate per 1.024 × 10-5 units Solution, α- amylase activity of the sample as a unit. Principle 4 α- β- amylase degradation limit dextrin substrate solution, the enzymatic reaction process, respectively, at different times aliquots of the reaction mixture was added iodine Solution. With the reaction time, the reaction mixture of iodine and reduce color intensity to determine enzyme activity.

5 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade. Test water line with GB/T 6682 in the three water requirements. 5.1 iodine. 5.2 potassium iodide. 5.3 calcium chloride. 5.4 anhydrous sodium acetate. 5.5 sulfuric acid. 5.6 glacial acetic acid. 5.7 soluble starch. NOTE. to use Lintner starch or of comparable quality domestic soluble starch. ......

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