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GB/T 42475-2023 English PDF

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GB/T 42475-2023: Chemicals - Apis cerana Fabricius acute oral toxicity test
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 42475-2023279 Add to Cart 3 days Chemicals - Apis cerana Fabricius acute oral toxicity test Valid

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Basic data

Standard ID: GB/T 42475-2023 (GB/T42475-2023)
Description (Translated English): Chemicals - Apis cerana Fabricius acute oral toxicity test
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A80
Classification of International Standard: 13.300; 13.020
Word Count Estimation: 14,112
Date of Issue: 2023-03-17
Date of Implementation: 2023-10-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 42475-2023: Chemicals - Apis cerana Fabricius acute oral toxicity test

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 13.300;13.020 CCSA80 National Standards of People's Republic of China Acute Oral Toxicity Test of Chemicals in Apis chinensis Released on 2023-03-17 2023-10-01 implementation State Administration for Market Regulation Released by the National Standardization Management Committee

foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules for Standardization Documents" drafting. Please note that some contents of this document may refer to patents. The issuing agency of this document assumes no responsibility for identifying patents. This document is proposed and managed by the National Standardization Technical Committee on Hazardous Chemicals Management (SAC/TC251). This document was drafted by. Eco-Environmental Research Center of the Chinese Academy of Sciences, Beijing Kerun Tianlang Environmental Technology Co., Ltd., CCIC Kejian (Tian Jin) Inspection and Testing Co., Ltd., China Academy of Inspection and Quarantine, Xiamen Yinchao Technology Co., Ltd., Guangdong Mingkai Technology Co., Ltd. Division, Zhejiang Huiri Environmental Testing Co., Ltd., Jinjiu Technology Co., Ltd. The main drafters of this document. Wan Bin, Wang Huili, Li Jianzhong, Xu Peng, Li Wei, Wang Yinghuan, Han Yongtao, Zhang Yanfeng, Jiang Fuping, Zhou Lili, Li Ji, Yi Zhonghui, Wan Qinglan, Mao Xiaoyao. Acute Oral Toxicity Test of Chemicals in Apis chinensis

1 Scope

This document specifies the test principle and test method for the determination of acute oral toxicity of chemicals to Chinese bees by using the small beaker method and the feeding tube method. method, calculation and presentation of results, quality assurance and quality control, and test reporting. This document is applicable to the determination of acute oral toxicity of chemicals to Apis sinensis using the small beaker method and the feeding tube method.

2 Normative references

The contents of the following documents constitute the essential provisions of this document through normative references in the text. Among them, dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document. GB 5749 Hygienic Standards for Drinking Water

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 acute toxicity Obvious adverse reactions produced by organisms exposed to a certain chemical substance once or more within a short period of time (1d~4d). Note. In this test, acute oral toxicity is expressed by the half-lethal concentration (LC50), that is, within a specific time (up to 96h) 50% of the tested honey Concentration of substance at which bees die. 4.Test principle Mix the test substance and honey water to form solutions of different concentrations to feed the Chinese bees. See Appendix A for the biological background. Take 48h as a test During the test period, record the number of dead bees at 24h and 48h, calculate the death rate of each group, and determine the test period when 50% of the test bees died. The concentration of the substance is represented by 24h-LC50 or 48h-LC50.If the research time is extended, continue to record the number of dead bees at 72h and 96h.

5 Test method

5.1 Instruments and equipment 5mL small beaker, temperature control and continuous temperature measurement equipment, humidifier and temperature and humidity meter, feeding tube, precision balance (at least accurate to ±0.1%). 5.2 Test vessel The test container can be a beehive with a length, width and height of 10cm, 10cm and 10cm, lined with gauze; or a constant temperature and humidity incubator, with Ensure that the test temperature and humidity are stable. When the test substance is volatile, the laboratory should be equipped with ventilation devices; when the test substance is not volatile , the bottom and top of the test container should be open. Clean the container before the test and dry it for later use. Randomly place the test containers in the test area
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