GB/T 41917-2022 English PDF

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GB/T 41917-2022: Nanotechnologies - Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 41917-2022	384	Add to Cart	4 days	Nanotechnologies - Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials	Valid

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Basic data

Standard ID: GB/T 41917-2022 (GB/T41917-2022)
Description (Translated English): Nanotechnologies - Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C04
Classification of International Standard: 07.120
Word Count Estimation: 22,232
Date of Issue: 2022-10-14
Date of Implementation: 2023-05-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 41917-2022: Nanotechnologies - Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Nanotechnologies -- Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials ICS 07.120 CCSC04 National Standards of People's Republic of China Nanotechnology Electron Spin Resonance (ESR) detection of metal oxide nanomaterials Generated reactive oxygen species (ROS) (ISO /T S18827.2017, IDT) Published on 2022-10-12 2023-05-01 Implementation State Administration for Market Regulation Released by the National Standardization Administration directory Preface III Introduction IV 1 Scope 1 2 Normative references 1 3 Terms, Definitions and Abbreviations 1 3.1 Terms and Definitions 1 3.2 Abbreviations 1 4 Principle 2 4.1 Overview 2 4.2 Spin trapping method 2 4.2.1 Overview 2 4.2.2 DMPO 2 4.2.3 BMPO 2 4.2.4 TPC 2 4.3 Free radical positive control 3 4.3.1 Fenton reaction [17] 3 4.3.2 Hypoxanthine/xanthine oxidase system[18] 3 4.3.3 Bengal red photosensitization [19,20] 3 5 Reagent 3 6 Instrument 4 7 Sample Preparation 4 7.1 Test Sample Preparation (Metal Oxide Nanomaterial Suspension) 4 7.2 Preparation of solutions for generating hydroxyl radicals 4 7.2.1 FeSO4 solution 4 7.2.2 H2O2 solution 4 7.3 Solution preparation for superoxide anion generation 4 7.3.1 Phosphate buffer 4 7.3.2 Hypoxanthine solution 4 7.3.3 Xanthine oxidase solution 5 7.4 Preparation of Singlet Oxygen Generation Solution 5 7.5 Preparation of spin traps 5 7.5.1 Overview 5 7.5.2 DMPO stock solution 5 7.5.3 BMPO stock solution 5 7.5.4 TPC stock solution 5 7.6 Reaction between test sample and spin trap 5 7.6.1 Overview 5 7.6.2 Reaction of DMPO 6 7.6.3 Reaction of BMPO 6 7.6.4 Reaction of TPC 6 7.7 Positive Control and Spin Trap Reaction 6 7.7.1 Formation of DMPO Radical Adducts (DMPO/·OH) 6 7.7.2 Formation of BMPO Radical Adducts (BMPO/·OOH) 6 7.7.3 Formation of TPC Radical Adducts (TPC/1O2) 6 7.8 Preparation of Standard Materials for Spin Calculations 6 8 Influencing factors 7 8.1 Sampling 7 8.2 Sampling time 7 9 Operation step 7 9.1 Overview 7 9.2 Sample injection 8 9.3 ESR testing 8 10 Example of test results 13 10.1 DMPO/·OH 13 10.2 BMPO/·OOH 14 10.3 TPC/1O2 14 10.4 TEMPOL 14 Reference 15

foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" drafted. This document is equivalent to ISO /T S18827.2017 "Nanotechnology Electron Spin Resonance (ESR) method for the detection of metal oxide nanomaterials Reactive Oxygen Species (ROS) produced by feedstocks. The file type is adjusted from ISO technical specifications to my country's national standards. The following minimal editorial changes have been made to this document. --- References reordered. Please note that some content of this document may be patented. The issuing agency of this document assumes no responsibility for identifying patents. This document is proposed by the Chinese Academy of Sciences. This document is under the jurisdiction of the National Nanotechnology Standardization Technical Committee (SAC/TC279). This document is drafted by. National Nanoscience Center, Xuchang University. The main drafters of this document. Wu Xiaochun, Ji Yinglu, Fan Huizhen, He Weiwei.

Introduction

In recent years, the application of metal and metal oxide nanomaterials in biomedicine and industry has increased dramatically. However, most man-made nano The scientific rationale for the cytotoxicity and genotoxicity of materials is not fully understood. The production of reactive oxygen species (ROS) is an important aspect of nanotoxicity mechanism. The research on the harmful effects of metal oxide nanomaterials is still in its infancy. ROS-generating ability is the result of metal oxide nanomaterials one of the main sources of toxicity. Excessive ROS can cause oxidative stress, resulting in the inability of cells to maintain normal physiological redox regulation functions. It can lead to DNA damage, dysregulation of cell signaling pathways, changes in cell migration, cytotoxicity, apoptosis and tumorigenesis. The generation of ROS depends on many key factors, including size, shape, particle surface, positive surface charge, surface functional groups, particle solubility, Metal ions released from nanometals and nanometal oxides, UV light activation, aggregation, mode of action with cells, inflammation, and mediator interactions pH[4]. Therefore, in order to detect and quantify ROS generated on the surface of metal oxide nanomaterials, electron spin resonance is proposed in this paper. (ESR) method. Among ROS, the most biologically relevant and widely studied are hydroxyl radical (OH), superoxide anion (O2-), singlet Oxygen (1O2) and hydrogen peroxide (H2O2). However, it is very difficult or impossible to directly detect some free radicals (such as superoxide anion and hydroxyl radicals) in solution at room temperature of [5]. The ESR spin trapping technique is a very useful tool to study transient free radicals [6]. developed in the late 1960s Spin trapping technology uses nitrones or nitro compounds (spin trapping agents) to react with target free radicals to form stable and identifiable free radicals. base (spin adduct), and then use ESR spectrometer to detect a technique. Spin adducts can be directly detected by ESR spectrometers. The ESR spectra of spin adducts are specific and can provide ROS presence fingerprint characteristics. This document specifies the detection of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) produced by metal oxide nanomaterials using ESR and adducts of hydroxyl radicals, 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO) and superoxide anions, to and a method for the adduct of 2,2,5,5-tetramethyl-3-pyrroline-3-carbamide (TPC) and singlet oxygen. This document provides a Methods for assessing ROS production from metal oxide nanomaterials in a cellular environment. The method may be in the evaluation stage of physicochemical properties and has not been carried out yet. Cytotoxicity tests of nanomaterials that provide valuable predictive information on ROS-mediated cytotoxicity. Nanotechnology Electron Spin Resonance (ESR) detection of metal oxide nanomaterials Generated reactive oxygen species (ROS)

1 Scope

This document describes a method for detecting reactive oxygen species generated from metal oxide nanomaterials in aqueous solution using electron spin resonance (ESR). (ROS)(·OH, O2- and 1O2). This document does not apply to ESR detection methods that do not use ROS-specific spin traps.

2 Normative references

There are no normative references in this document. 3 Terms, Definitions and Abbreviations 3.1 Terms and Definitions The following terms and definitions apply to this document. 3.1.1 nanomaterial Any material whose external dimension, internal or surface structure is on the nanoscale. Note 1.This generic term includes nano-objects and nano-structured materials. Note 2.See ISO /T S80004-1.2015, 2.8-2.10. [Source. ISO /T S80004-1.2015, 2.4] 3.1.2 test sample testsample Materials, devices, parts of devices, components, extracts or parts thereof for use in biological testing, chemical testing or evaluation. [Source. GB/T 16886.5-2017, 3.5] 3.1.3 zero baseline control zerobaselinecontrol Equivalent to a positive control when no free radicals are detected. Note. For example, the zero baseline control of the Fenton reaction positive control is a mixed solution of iron-free H2O2 and DMPO; hypoxanthine-xanthine oxidase (HX- XO) system is hypoxanthine and BMPO without HX-XO; Bengal red photosensitization system is Bengal red and TPC without light conditions. 3.1.4 positive control positivecontrol Well-characterized materials and/or substances. The material and/or substance, when used in the evaluation of a specified test method, demonstrates that the test procedure The sequence is suitable for obtaining a reproducible, appropriately positive or reactive response in the test system. [Source. GB/T 16886.12-2017, 3.12] 3.2 Abbreviations The following abbreviations apply to this document. ......

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