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GB/T 41907-2022 PDF English

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GB/T 41907-2022: Assay method of enterokinase activity
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GB/T 41907-2022: Assay method of enterokinase activity

---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT41907-2022
GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA ICS 07.080 CCS C 27 Assay method of enterokinase activity ISSUED ON: OCTOBER 12, 2022 IMPLEMENTED ON: OCTOBER 12, 2022 Issued by: State Administration for Market Regulation; Standardization Administration of the PRC.

Table of Contents

Foreword ... 3 Introduction ... 4 1 Scope ... 5 2 Normative references ... 5 3 Terms and definitions ... 5 4 Reagents or materials ... 5 5 Apparatus ... 6 6 Test procedure ... 6 7 Result calculation ... 7 8 Quality control ... 7

Foreword

This document is drafted in accordance with GB/T 1.1-2020 "Directives for standardization - Part 1: Rules for the structure and drafting of standardizing documents". Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. The issuing authority of this document shall not be held responsible for identifying any or all such patent rights. This document was proposed by and shall be under the jurisdiction of National Tool Enzyme Standardization Working Group (SAC/SWG 11). Drafting organizations of this document: Leigu (Xiamen) Biomedicine Co., Ltd.; General Biosystems (Anhui) Co., Ltd.; Suzhou Kunqi Biotechnology Co., Ltd.; Xiahe (Shenzhen) Biotechnology Co., Ltd.; Xiamen Yinxiang Group Co., Ltd.; Xiamen Zhong Ji Xin Testing Technology Co., Ltd.; Langfang Biotecnovo Medical Laboratory Co., Ltd.; Fujian Nansheng Technology Co., Ltd.; Shanghai Yingzi Life Technology Co., Ltd.; Suzhou Beaver Biomedical Engineering Co., Ltd.; Angel Yeast Co., Ltd.; Shandong University; Fujian Agriculture and Forestry University; Fudan University; Shanghai Chuyu Biotechnology Co., Ltd.; Division of Chemical Metrology and Analytical Science, National Institute of Metrology, China; Shenzhen BGI Life Science Research Institute. Main drafters of this document: Huang Facan, Zheng Dengzhong, Yong Jingui, Zhao Yi, Zhang Zhigang, Jiang Feng, Pang Tieliang, Zhu Li, Zhong Jiang, Yao Juan, Liu Bin, Chen Xiulan, Ren Hui, Xing Zhigang, Zhao Chao, Quan Can, Zeng Feng, Zhang Wenwei, Dong Yuliang, Xi Feng, Cheng Lei. Assay method of enterokinase activity

1 Scope

This document describes the reagents or materials, apparatus, test procedure, result calculation, and quality control for the assay method of enterokinase activity. This document applies to the assay of enterokinase activity.

2 Normative references

The contents of the following documents, through normative references in this text, constitute indispensable provisions of this document. Among them, for dated references, only the edition corresponding to that date applies to this document. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Water for analytical laboratory use - Specification and test methods

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Enterokinase A proteolytic enzyme secreted by duodenal cells, which can cleave the lysine carboxy- terminal peptide bond containing 4 aspartic acids (aspartic acid-aspartic acid-aspartic acid-aspartic acid-lysine). 3.2 Activity unit of enterokinase (U) The amount of enzyme - required to degrade 95% of 0.5 mg of Thioredoxin-NP-27 to NP-27 within 16 h at 37 °C - is 1 activity unit of enterokinase (U).

4 Reagents or materials

Unless otherwise specified, the reagents used in this method shall be of analytical grade. All reagent solutions should be prepared in large volumes; subpackaged in small volumes and sterilized by autoclaving before use. Reagents that are not suitable for ......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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