GB/T 41906-2022 English PDF

US$119.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 41906-2022: Assay method of superoxide dismutase activity
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 41906-2022	119	Add to Cart	3 days	Assay method of superoxide dismutase activity	Valid

Similar standards

GB/T 41907   GB/T 44828   

Basic data

Standard ID: GB/T 41906-2022 (GB/T41906-2022)
Description (Translated English): Assay method of superoxide dismutase activity
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C27
Classification of International Standard: 07.080
Word Count Estimation: 6,614
Date of Issue: 2022-10-14
Date of Implementation: 2022-10-12
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 41906-2022: Assay method of superoxide dismutase activity

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Assay method of superoxide dismutase activity ICS 07.080 CCSC27 National Standards of People's Republic of China Superoxide dismutase activity detection method Published on 2022-10-12 2022-10-12 Implementation State Administration for Market Regulation Released by the National Standardization Administration directory Preface I Introduction II 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Principle 1 5 Reagents or materials 1 6 Instruments 2 7 Sample preparation 2 8 Test Step 2 9 Result calculation 3 10 Quality Control 3

foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" drafted. Please note that some content of this document may be patented. The issuing agency of this document assumes no responsibility for identifying patents. This document is proposed and managed by the National Tool Enzyme Standardization Working Group (SAC/SWG11). This document is drafted by. Leigu (Xiamen) Biopharmaceutical Co., Ltd., Xiahe (Shenzhen) Biotechnology Co., Ltd., Guangdong Zhongding Testing Technology Co., Ltd., Anhui Public Inspection Research Institute Co., Ltd., Bengbu Product Quality Supervision and Inspection Research Institute, Suzhou Kunqi Biotechnology Co., Ltd. Co., Ltd., Shenzhen Hudaguao Biotechnology Co., Ltd., Fujian Nansheng Technology Co., Ltd., Shanghai Yingzi Life Technology Co., Ltd., Fujian Huacan Pharmaceutical Co., Ltd., Shandong University, Fujian Agriculture and Forestry University, Fudan University, Suzhou Beaver Biomedical Engineering Co., Ltd., Xiamen University, Shanghai Chuyu Biotechnology Co., Ltd., Wuhan University of Science and Technology, Institute of Metrology and Analysis of China Institute of Metrology. The main drafters of this document. Huang Facan, Zheng Dengzhong, Wang Tongzhen, Tong Chengliang, Zhao Yi, Tang Qingwen, Feng Xiang, Liu Bin, Zhong Jiang, Zhu Li, Yao Juan, Ren Hui, Zhang Yongyou, Xing Zhigang, Yang Zhonghua, Quan Can.

Introduction

Superoxide dismutase (EC1.15.1.1, referred to as SOD) is a metalloenzyme widely present in aerobic microorganisms, animals and plants, which can catalyze The superoxide anion radical undergoes a disproportionation reaction. 2O2·- 2H → H2O2 O2.SOD maintains superoxide anion freedom in living organisms It plays an important role in the dynamic balance of base generation and elimination. To formulate national standards for the detection method of superoxide dismutase activity to promote the The industrialization of tool-like enzymes is of great significance for the production and use of superoxide dismutase. Superoxide dismutase activity detection method

1 Scope

This document describes the principles, reagents or materials, equipment, sample preparation, test procedures, Results calculation and quality control. This document applies to the detection of superoxide dismutase activity.

2 Normative references

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, dated citations documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Experimental Methods

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 superoxide dismutase Metalloenzymes are widely present in aerobic microorganisms, animals and plants, and can catalyze the disproportionation of superoxide anion free radicals. Note. SOD plays an important role in maintaining the dynamic balance of the production and elimination of superoxide anion free radicals in organisms. 3.2 At 25°C, the amount of enzyme that inhibits the auto-oxidation rate of pyrogallol by 50% per minute in 1 mL of the reaction solution is one superoxide dismutase activity. unit (U).

4 Principles

Pyrogallol is stable in an acidic environment, but will undergo auto-oxidation in a weakly alkaline environment, usually when a molecule of oxygen is completely reduced. 4 electrons generate 2O2- to further generate water. While pyrogallol only accepts one electron during auto-oxidation to generate superoxide anion free base, and produce colored intermediates at a certain rate during its auto-oxidation process. SOD can disproportionate O2- into H2O2 and O2, thereby inhibiting the auto-oxidation rate of pyrogallol, so the activity of the enzyme can be indirectly estimated based on the ability of SOD to decompose O2.

5 Reagents or materials

Unless otherwise specified, the reagents used are of analytical grade, and the water is secondary water specified in GB/T 6682. 5.1 A solution. 0.1moL/L hydrochloric acid solution Measure 4.166mL of hydrochloric acid, add water to make up to 500mL. ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 41906-2022_English be delivered?

Answer: Upon your order, we will start to translate GB/T 41906-2022_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 41906-2022_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 41906-2022_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.