GB/T 41726-2022 English PDF

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GB/T 41726-2022: Identification and detection methods of monomer ginsenoside
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Basic data

Standard ID: GB/T 41726-2022 (GB/T41726-2022)
Description (Translated English): Identification and detection methods of monomer ginsenoside
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B04
Classification of International Standard: 65.020.01
Word Count Estimation: 38,348
Date of Issue: 2022-10-14
Date of Implementation: 2023-05-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 41726-2022: Identification and detection methods of monomer ginsenoside

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification and detection methods of monomer ginsenoside ICS 65.020.01 CCSB04 National Standards of People's Republic of China Identification and detection method of ginseng monomer saponins Published on 2022-10-12 2023-05-01 Implementation State Administration for Market Regulation Released by the National Standardization Administration directory Preface I Introduction II 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Basic information and structural formulas of 25 ginseng monomer saponins1 4.1 Basic information 1 4.2 Structure 4 5 Principles of the method 7 5.1 The principle of melting point 7 5.2 Principle of Specific Optical Rotation 7 5.3 Principles of thin-layer chromatography 7 5.4 Principles of mass spectrometry 7 5.5 Principles of Nuclear Magnetic Resonance 8 5.6 Principles of HPLC 8 6 Environmental conditions8 7 Reagents and Materials 8 8 Instruments 8 9 Identification and detection methods8 9.1 Sensory detection methods 8 9.2 Physical and chemical constant detection method 9 9.3 Qualitative detection methods 11 9.4 Quantitative detection methods 19 Appendix A (informative) 13C-NMR data of ginseng monomer saponins 20 Appendix B (normative) Quantitative detection method of ginseng monomer saponins (HPLC) 33

foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" drafted. Please note that some content of this document may be patented. The issuing agency of this document assumes no responsibility for identifying patents. This document is proposed and managed by the National Technical Committee for Standardization of Ginseng Products (SAC/TC403). This document is drafted by. Jilin Ginseng Research Institute, National Ginseng Product Quality Supervision and Inspection Center, Hangzhou Huqingyutang National Medicine Co., Ltd. Division, Liaoning Province Ginseng Product Quality Supervision and Inspection Center, Tonghua City Product Quality Inspection Institute, Baishan City Product Quality Inspection Institute, Zhejiang Shouxiangu Medical Center Pharmaceutical Co., Ltd., Jilin Provincial Horticultural Specialty Management Station, Jilin Provincial Institute of Standards, China National Institute of Standardization, College of Chemistry, Jilin University, Tonghua Normal University Ginseng College. The main drafters of this document. Cao Zhiqiang, Xu Fangfei, Wu Lunpeng, Yang Zhongying, Liu Lifeng, Ci Hui, Tang Chongming, Li Zhenhao, Li Lei, Feng Jia, Zhang Hongjie, Chu Qiao, Xie Lijuan, Kang Chenkai, Preliminary Jun, Xu Jing, Ma Qingshu, Shan Jiping, Tian Jingxin, Jiang Lingxia, Yang Huailei, Xi Xingjun, Zhang Honggui, Hu Yanwu, Lou Ziheng, Wang Zhicai, Sun Benchang, Xie Zengyou, Long Yan, Wang Guoming, Guo Changbing, Chi Meili, Ma Zhenghe, Guo Deshan, Song Yingying, Yue Lele.

Introduction

This document is the identification and detection method standard of 25 commonly used ginseng monomer saponins, including 20(S)-protopanaxadiol, panaxadiol, 20(S)-protopanaxatriol, panaxatriol, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rc, ginsenoside Rd, ginsenoside F1, ginsenoside F2, ginsenoside Rf, 20(S)-ginsenoside Rh1, 20(R)- Ginsenoside Rh1, 20(S)-ginsenoside Rg2, 20(R)-ginsenoside Rg2, 20(S)-ginsenoside Rh2, 20(R)-ginsenoside Rh2, Ginsenoside CK, ginsenoside Ro, pseudoginsenoside F11, 20(S)-ginsenoside Rg3, 20(R)-ginsenoside Rg3, etc. With the continuous deepening of experimental research, this document will add new ginseng monomer saponins in due course. Identification and detection method of ginseng monomer saponins

1 Scope

This document describes the principles of the identification and detection of 25 commonly used ginseng monomer saponins, the basic information and structures of ginseng monomer saponins formula, environmental conditions, reagents and materials, equipment, identification and detection methods. This document applies to the identification and detection of ginseng monomer saponins.

2 Normative references

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, dated citations documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Test Methods GB/T 26792 High Performance Liquid Chromatograph The Fourth Pharmacopoeia of the People's Republic of China

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 A method for identifying the measured substances by using technical methods such as moisture, melting point and specific rotation. 3.2 Detection methods for determining the types, properties, characteristics, and structural composition of substances by using relevant analytical techniques. 3.3 A detection method for accurately determining the content of components (elements or groups) in a substance or the purity of a substance by using relevant analytical techniques. 4 Basic information and structural formulas of 25 ginseng monomer saponins 4.1 Basic information The basic information of ginseng monomer saponins is shown in Table 1. ......

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