GB/T 41712-2022 English PDF

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GB/T 41712-2022: Assay method of deoxyribonuclease I activity and impurity
Status: Valid

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Basic data

Standard ID: GB/T 41712-2022 (GB/T41712-2022)
Description (Translated English): Assay method of deoxyribonuclease I activity and impurity
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C27
Classification of International Standard: 07.080
Word Count Estimation: 10,114
Date of Issue: 2022-10-14
Date of Implementation: 2023-05-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 41712-2022: Assay method of deoxyribonuclease I activity and impurity

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Assay method of deoxyribonuclease I activity and impurity ICS 07.080 CCSC27 National Standards of People's Republic of China Deoxyribonuclease I enzyme activity and impurity detection method Published on 2022-10-12 2023-05-01 Implementation State Administration for Market Regulation Released by the National Standardization Administration directory Preface III Introduction IV 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Reagents or materials 1 5 Instruments 2 6 Test Step 2 7 Quality Control5

foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" drafted. Please note that some content of this document may be patented. The issuing agency of this document assumes no responsibility for identifying patents. This document is proposed and managed by the National Tool Enzyme Standardization Working Group (SAC/SWG11). This document is drafted by. Fujian Nansheng Technology Co., Ltd., Xiahe (Shenzhen) Biotechnology Co., Ltd., Shenzhen Zhongding Testing Technology Co., Ltd. Co., Ltd., Suzhou Kunqi Biotechnology Co., Ltd., Bengbu Product Quality Supervision and Inspection Institute, Nanjing Novizan Biotechnology Co., Ltd. Company, Xi'an Guolian Quality Inspection Technology Co., Ltd., Fujian Huacan Pharmaceutical Co., Ltd., Shanghai Yingzi Life Technology Co., Ltd., Leigu (Xiamen) Biopharmaceutical Co., Ltd., Beijing Zhongtian Biaoke Standardization Technology Research Institute Co., Ltd., Fudan University, Xiamen Zhishan Biotechnology Co., Ltd. Company, Shandong University, Angel Yeast Co., Ltd., Shanghai Chuyu Biotechnology Co., Ltd., Wuhan University of Science and Technology, China Metrology Research Institute Institute of Metrology and Analytical Sciences, Xiamen Aide Biomedical Technology Co., Ltd. The main drafters of this document. Huang Facan, Zheng Dengzhong, Kan Guanglei, Zhao Yi, Guo Qing, Wang Cui, Zhou Gaohuai, Zhong Jiang, Zhu Li, Chen Xiulan, Yao Juan, Song Najie, Quan Can, Xu Wenlai, Xing Zhigang, Yang Zhonghua, Li Chao.

Introduction

Deoxyribonuclease I (DNaseI, EC3.1.21.1) is the most representative endonuclease, which degrades single- and double-stranded DNA and produces Produces a decomposition product with a 5'-phosphate end, and its decomposition product contains a single nucleotide or an oligonucleotide of 8 to 12 bases under normal conditions acid, with an average size of about 4 nucleotides. Formulate the national standard for DNAzyme I to promote the industrialization of this type of tool enzyme. The production and use of oxyribonuclease I is of great significance. Deoxyribonuclease I enzyme activity and impurity detection method

1 Scope

This document describes the detection methods for deoxyribonuclease I enzyme activity and impurities. This document is applicable to the detection of deoxyribonuclease I enzyme activity and impurities.

2 Normative references

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, dated citations documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Experimental Methods

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 deoxyribonuclease Nucleases that hydrolyze phosphodiester bonds in deoxyribonucleic acid (DNA) to generate oligonucleotides or mononucleotides. 3.2 deoxyribonuclease I deoxyribonuclease I Hydrolysis of single- and double-stranded DNA to generate intranucleic acid mononucleotides or oligonucleotides (8-12 bases) with 5'-phosphate ends Dicer. 3.3 Using calf thymus DNA as a substrate, in a solution at 25°C, pH 5.0, the absorbance changes by 0.001 per milliliter per minute at 260 nm The degree value is one unit of enzyme activity (kunitzunit). 3.4 Impurity Other endonucleases or exonucleases that affect the substrate.

4 Reagents or materials

4.1 Water Comply with the secondary water specified in GB/T 6882. 4.2 1mol/L sodium acetate buffer Accurately weigh 8.2 g of anhydrous sodium acetate, dissolve it in water, and adjust the pH to 5.0 with 5 mol/L HCl. After mixing, the volume is adjusted to 100 mL. ......

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