GB/T 40448-2021 English PDF

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GB/T 40448-2021: Detection and identification of ergot
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 40448-2021	359	Add to Cart	4 days	Detection and identification of ergot	Valid

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Basic data

Standard ID: GB/T 40448-2021 (GB/T40448-2021)
Description (Translated English): Detection and identification of ergot
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 18,137
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40448-2021: Detection and identification of ergot

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of ergot ICS 65.020.01 CCSB16 National Standards of People's Republic of China Ergot quarantine and identification method Released on 2021-08-20 2022-03-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed and managed by the National Plant Quarantine Standardization Technical Committee (SAC/TC271). Drafting organizations of this document. Wuhan Customs Technology Center, Nanjing Customs Animal, Plant and Food Testing Center, Changchun Customs Technology Center, China Quality Wuhan Branch of the Quantitative Certification Center. The main drafters of this document. Wang Zhenhua, Wang Qin, Peng Chao, Liu Xiaoyu, Zhao Hui, Li Qiao, Zeng Xiandong, Zheng Jian, Xu Xue, Wu Cuiping, Wei Chunyan. Ergot quarantine and identification method

1 Scope

This document describes the quarantine and identification methods for ergot. This document is applicable to the quarantine and identification of ergot in plants and their products.

2 Normative references

There are no normative references in this document.

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Basic information of ergot

Scientific name. ClavicepsTul. English name. ergot Taxonomic status. Fungi, Ascomycota, Ascomycotina, Pezizomyco- tina, Sordariomycetes, Hypocreales, Clavicipaceae, Claviceps. Asexual generation belongs to asexual fungi Deuteromycetes, Moniliales, Tuberculariaceae, and Meliaceae SphaceliaLeveile. There are more than 40 species of ergot, the most common is Claviceps purpurea, which has the widest host range. Transmission route. Plant disease residues, infected seeds, sclerotia, etc. are the main routes of long-distance transmission, and spores are caused by wind, rain or insects to invade again Dyed and popular. See Appendix A for additional information on ergot.

5 Principles of the method

According to the morphological characteristics of ergot sclerotia, the morphology of colonies, hyphae, conidia, ascus shells, ascos and ascospores grown on the medium are combined The PCR amplified fragment size and sequencing results are used to determine the results.

6 Instruments and main reagents

6.1 Equipment Biological microscope (with measurement function), stereo microscope, PCR amplification instrument, gel imaging system, electrophoresis instrument, ultra-clean workbench, high-speed cooling Freezing centrifuge, pure water meter, autoclave, ice maker, light incubator, balance, pH meter, water bath, ultra-low temperature refrigerator, conventional refrigerator, vortex Oscillator. 6.2 Main reagents Unless otherwise specified, all reagents are analytical reagents or biochemical reagents. ......

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