GB/T 40368-2021 English PDF

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GB/T 40368-2021: Determination of kunitz trypsin inhibitor of plant metabolites - Enzyme-linked immunosorbent assay
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 40368-2021	119	Add to Cart	3 days	Determination of kunitz trypsin inhibitor of plant metabolites - Enzyme-linked immunosorbent assay	Valid

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Basic data

Standard ID: GB/T 40368-2021 (GB/T40368-2021)
Description (Translated English): Determination of kunitz trypsin inhibitor of plant metabolites - Enzyme-linked immunosorbent assay
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A40
Word Count Estimation: 6,685
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40368-2021: Determination of kunitz trypsin inhibitor of plant metabolites - Enzyme-linked immunosorbent assay

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of kunitz trypsin inhibitor of plant metabolites - - Enzyme-linked immunosorbent assay ICS 07.080 CCSA40 National Standards of People's Republic of China Determination of trypsin inhibitor of plant metabolites Enzyme-linked immunosorbent assay Released on 2021-08-20 2022-03-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed and managed by the National Standardization Technical Committee for Biochemical Testing (SAC/TC387). Drafting organizations of this document. Jiangnan University, Beijing Technology and Business University, Beijing Sambo Technology Co., Ltd., Lishui Lancheng Agricultural Science Testing Technology Co., Ltd. company. The main drafters of this document. Ma Aijin, Kuang Hua, Hao Shuai, Liu Liqiang, Xu Xinxin, Lin Lu, Zhang Shuqi. Determination of trypsin inhibitor of plant metabolites Enzyme-linked immunosorbent assay

1 Scope

This document specifies the enzyme-linked immunosorbent assay method for plant metabolite trypsin inhibitor. This document is applicable to the determination of trypsin inhibitor of the metabolite of soybean and its products.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to This document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Principle

After the sample is processed, the trypsin inhibitor in the sample solution is bound to the trypsin inhibitor antibody (capture antibody) coated in the microplate. Combine, wash the plate to remove unbound other components, and then combine with the enzyme-labeled antibody of trypsin inhibitor, add the enzyme-labeled substrate to develop the color, and use the microplate reader Measure the absorbance value at 450nm wavelength and calculate the trypsin inhibitor content.

5 Reagents and materials

Unless otherwise specified, the chemical reagents used in this method are analytically pure, and the water is secondary water specified in GB/T 6682. 5.1 Trypsin Inhibitor Detection Kit. See Appendix A. 5.2 Trypsin inhibitor. purity ≥95%, CAS number is 9035-81-8. 5.3 The required solution should be prepared in accordance with the instructions of the kit.

6 Equipment

6.1 Small grinder. 6.2 Sample sieve. the aperture is 0.9mm. 6.3 Homogenizer. 6.4 Vortex mixer. 6.5 Electronic balance. a sense of 0.01g. 6.6 Centrifuge tube with stopper. 15mL. ......

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