GB/T 38578-2020 English PDF

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GB/T 38578-2020: Rapid determination of the allergenic protein from aquatic animals origin - Capillary electrophoresis
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 38578-2020	149	Add to Cart	3 days	Rapid determination of the allergenic protein from aquatic animals origin - Capillary electrophoresis	Valid

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Basic data

Standard ID: GB/T 38578-2020 (GB/T38578-2020)
Description (Translated English): Rapid determination of the allergenic protein from aquatic animals origin - Capillary electrophoresis
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A21
Classification of International Standard: 07.080
Word Count Estimation: 8,829
Date of Issue: 2020-03-31
Date of Implementation: 2020-03-31
Quoted Standard: GB/T 6682; GB/T 30891
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration
Summary: This standard specifies the method for the determination of allergenic proteins from aquatic products by capillary electrophoresis. This standard applies to the rapid detection of crustacean tropomyosin, crustacean arginine kinase and fish parvalbumin content.

GB/T 38578-2020: Rapid determination of the allergenic protein from aquatic animals origin - Capillary electrophoresis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Rapid determination of the allergenic protein from aquatic animals origin--Capillary electrophoresis ICS 07.080 A21 National Standards of People's Republic of China Rapid detection of allergenic proteins of aquatic origin Capillary electrophoresis 2020-03-31 release 2020-03-31 Implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by China National Institute of Standardization. This standard was drafted by. China National Institute of Standardization, Greentown Agricultural Testing Technology Co., Ltd., Zhejiang Technology and Business University, Beijing Technology and Business University, Beijing Sambe Technology Co., Ltd., Jimei University, Hunan Agricultural University, Wenzhou University, Rongcheng Huatong Marine Biotechnology Co., Ltd. The main drafters of this standard. Ma Aijin, Wang Chuanpi, He Xiaoming, Zhou Jinru, Fu Linglin, Jia Yingmin, Wu Qi, Hao Shuai, Liu Guangming, Zhou Hui, Chen Xiaojing, Wang Chong, Liu Yixiang, Liu Zhongfu, Wang Yanbo. Rapid detection of allergenic proteins of aquatic origin Capillary electrophoresis

1 Scope

This standard specifies the method for determination of aquatic allergenic proteins by capillary electrophoresis. This standard applies to the rapid detection of the content of crustaceous myosin, crustaceous arginine kinase and fish albumin.

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis laboratory specifications and test methods GB/T 30891 Standard for Sampling of Aquatic Products

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Allergenic protein A protein that can cause an allergic reaction in the body.

4 Principle

The allergenic protein in the sample is extracted by protein extract and purified by anion exchange chromatography, measured by capillary electrophoresis, migration time Qualitative, quantitative by external standard method.

5 Reagents or materials

Unless otherwise specified, only analytical reagents are used. 5.1 Water Grade 1 water specified in GB/T 6682. 5.2 Methanol Chromatographically pure. 5.3 Tris-hydroxymethylaminomethane-hydrochloric acid buffer Trimethylolaminomethane 1.21g, add 900mL of double-distilled water to dissolve, adjust the pH to 7.5 with 6mol/L hydrochloric acid solution, set with distilled water Make up to 1000mL, filter with 0.45μm filter membrane, ultrasonically shake (power.200W) for 30min, and store at room temperature. ......

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