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GB/T 38569-2020: Performance evaluation of industrial microorganism strain - Raman spectroscopy Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure Status: Valid
Similar standardsGB/T 38569-2020: Performance evaluation of industrial microorganism strain - Raman spectroscopy---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT38569-2020GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA ICS 07.080 A 21 Performance evaluation of industrial microorganism strain - Raman spectroscopy ISSUED ON: MARCH 31, 2020 IMPLEMENTED ON: MARCH 31, 2020 Issued by: State Administration for Market Regulation; Standardization Administration of the People’s Republic of China. Table of ContentsForeword ... 3 1 Scope ... 4 2 Normative references ... 4 3 Terms and definitions ... 4 4 Principle ... 4 5 Reagents or materials ... 5 6 Instruments and apparatuses ... 5 7 Determination steps ... 5 8 Result calculation and analysis ... 7 Performance evaluation of industrial microorganism strain - Raman spectroscopy1 ScopeThis Standard specifies the method for performance evaluation of industrial microorganism strain by Raman spectroscopy. This Standard applies to the performance consistency evaluation of bacteria, fungi, and microalgae for industrial fermentation relative to the reference strain.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only the dated version applies to this document. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682, Water for analytical laboratory use - Specification and test methods3 Terms and definitionsThe following terms and definitions are applicable to this document. 3.1 Performance evaluation of industrial microorganism strain Use Raman spectroscopy to carry out performance consistency evaluation of industrial microorganism strain. 3.2 Reference strain The identified stock strain, whose fermentation performance is clear, for fermentation production.4 PrincipleThe superimposed information of the Raman spectrum of the compound inside the cell can reflect the comprehensive phenotype of the cell. By comparing the similarity of the single-cell Raman spectrum of the to-be-evaluated strain with the reference strain, it can be judged whether the to-be-evaluated strain has comparable fermentation performance with the reference strain. Use a micro-pipette to respectively transfer 200 μL of the activated reference strain and the to-be-evaluated strain; respectively inoculate them into 3 containers that contain 20 mL of liquid medium; select the corresponding temperature and speed according to the production process parameters; cultivate to the stable period. 7.3 Sample preparation 7.3.1 Respectively transfer 1 mL of the cultured reference strain and the to-be- evaluated strain into a sterile tube. 7.3.2 Select the corresponding speed for centrifugation; discard the supernatant; add 1 mL of sterile double-distilled water; mix the bacteria. 7.3.3 Repeat 2 times in accordance with 7.3.2; transfer 10 μL of bacterial solution to a sterile tube; add 990 μL of sterile double-distilled water; mix well; pipette 2 μL of diluted bacterial solution; place it in a clean calcium fluoride glass slide for sample application; air-dry at room temperature in a sterile environment. 7.4 Measurement 7.4.1 Place the air-dried calcium fluoride glass slide on the micro Raman spectrometer stage; select a single cell in the transmissive light field mode; switch to the spectrum acquisition mode; measure the Raman spectrum signal. 7.4.2 Select at least 20 single cells randomly from each sample as the sample signal. 7.4.3 In the sample application area, collect 3 acellular signals; calculate the average value as the background signal. 7.5 Data processing 7.5.1 Perform background signal subtraction (sample signal minus background signal), baseline correction ( >5th order function) and area-based normalization of the raw data of single-cell Raman spectrum. 7.5.2 Calculate the signal-to-noise ratio, where the signal uses the signal intensity corresponding to the sharp Raman peak at 1 001 cm-1; discard data whose signal-to-noise ratio is less than 3; supplement the number of data, so that the to-be-evaluated strain group and the reference strain group have the same number of data. ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. 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