GB/T 38482-2021 English PDF

US$139.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 38482-2021: Determination of animal type I collagen - Polyacrylamide gel electrophoresis
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 38482-2021	139	Add to Cart	3 days	Determination of animal type I collagen - Polyacrylamide gel electrophoresis	Valid

Similar standards

GB/T 38484   GB/T 38480   GB/T 38481   GB/T 38479   GB/T 38478   

Basic data

Standard ID: GB/T 38482-2021 (GB/T38482-2021)
Description (Translated English): Determination of animal type I collagen - Polyacrylamide gel electrophoresis
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A21
Word Count Estimation: 6,690
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 38482-2021: Determination of animal type I collagen - Polyacrylamide gel electrophoresis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of animal type Ⅰ collagen -- Polyacrylamide gel electrophoresis ICS 07:080 A21 National Standards of People's Republic of China Determination of animal-derived type I collagen composition polyacrylamide gel electrophoresis Published on 2021-12-31 2022-07-01 Implementation State Administration for Market Regulation Released by the National Standardization Administration

foreword

This standard was drafted in accordance with the rules given in GB/T 1:1-2009: This standard is proposed and managed by China National Institute of Standardization: This standard was drafted by: Yantai Institute of Coastal Zones, Chinese Academy of Sciences, China National Institute of Standardization, Shanghai Ocean University, China Ocean University Science, Shandong Hongbo Standardization Service Office, and Institute of Process Engineering, Chinese Academy of Sciences: The main drafters of this standard: Qin Song, Li Jie, Wu Wenhui, Hou Hu, Ma Aijin, Cui Yulin, Chen Jun, Han Chunmei, Huang Yongdong: Determination of animal-derived type I collagen composition polyacrylamide gel electrophoresis

1 Scope

This standard specifies a method for determining the composition of animal-derived type I collagen by polyacrylamide gel electrophoresis (SDS-PAGE): This standard applies to the determination of type I collagen components in animal-derived collagen products:

2 Normative references

The following documents are essential for the application of this document: For dated references, only the dated version applies to this article pieces: For undated references, the latest edition (including all amendments) applies to this document: GB/T 6682 Analysis Laboratory Water Specifications and Test Methods Pharmacopoeia of the People's Republic of China (2015 Edition)

3 Terms and Definitions

The following terms and definitions apply to this document: 3:1 Collagen Three peptide chains of left-handed helical repeating Gly-XY sequence prepared from purified (non-crosslinked) type I collagen Structural protein coiled into a right-handed supercoiled structure characteristic: Note: Gly is glycine, X is usually proline, and Y is usually hydroxyproline or hydroxylysine: 3:2 typeⅠcollagen Collagen with a complete triple helix structure composed of 2 α1 chains and 1 α2 peptide chain:

4 Principles

The use of type I collagen has a triple helix structure that other proteins do not have, and with the action of specific collagenase, through Coomassie Brilliant blue was used to determine the staining limit of bovine serum albumin (BSA), and the composition of type Ⅰ collagen was determined by SDS-PAGE:

5 Reagents or materials

Unless otherwise specified, only analytical grade reagents were used: 5:1 Bacterial collagenase: 5:2 Bovine serum albumin (BSA) standard: 5:3 Water: It should meet the first-class water regulations in GB/T 6682: 5:4 20mmol/L sodium dihydrogen phosphate solution, pH7:4: ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 38482-2021_English be delivered?

Answer: Upon your order, we will start to translate GB/T 38482-2021_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 38482-2021_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 38482-2021_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.