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GB/T 38478-2021 English PDF

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GB/T 38478-2021: Determination of astaxanthin optical isomers content - Liquid chromatography
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 38478-2021159 Add to Cart 3 days Determination of astaxanthin optical isomers content - Liquid chromatography Valid

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Basic data

Standard ID: GB/T 38478-2021 (GB/T38478-2021)
Description (Translated English): Determination of astaxanthin optical isomers content - Liquid chromatography
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A21
Word Count Estimation: 7,746
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 38478-2021: Determination of astaxanthin optical isomers content - Liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of astaxanthin opticalisomers content -- Liquid chromatography ICS 07:080 CCSA21 National Standards of People's Republic of China Determination of optical isomer content of astaxanthin liquid chromatography Published on 2021-12-31 2022-07-01 Implementation State Administration for Market Regulation Released by the National Standardization Administration

foreword

This document is in accordance with the provisions of GB/T 1:1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" drafted: This document is proposed and managed by China National Institute of Standardization: This document is drafted by: Yantai Institute of Coastal Zones, Chinese Academy of Sciences, Institute of Oceanography, Chinese Academy of Sciences, China National Institute of Standardization, Shandong Provincial Institute of Standardization, Yunnan Longbray Biotechnology Co:, Ltd:, Fujian Normal University Fuqing Branch, and Institute of Process Engineering, Chinese Academy of Sciences: The main drafters of this document: Liu Jianguo, Qin Song, Su Fang, Han Chunmei, Liang Wenwei, Cui Yulin, Ma Aijin, Chen Jun, Wang Yinchu, Huang Yongdong: Determination of optical isomer content of astaxanthin liquid chromatography

1 Scope

This document specifies a method for the determination of optical isomer content in astaxanthin by liquid chromatography: This document is applicable to the determination of the optical isomer content of astaxanthin in astaxanthin products:

2 Normative references

The contents of the following documents constitute essential provisions of this document through normative references in the text: Among them, dated citations documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to this document: GB/T 6682 Analysis Laboratory Water Specifications and Test Methods

3 Terms and Definitions

There are no terms and definitions that need to be defined in this document:

4 Principles

After grinding and leaching the astaxanthin in the sample, it was enzymatically hydrolyzed by cholesterol esterase to obtain free astaxanthin, which was separated by a chiral chromatographic column and used to obtain free astaxanthin: It was determined by liquid chromatograph equipped with UV detector and quantified by external standard method:

5 Reagents or materials

Unless otherwise specified, only analytical grade reagents were used: 5:1 Water It should comply with the secondary water regulations in GB/T 6682: 5:2 Acetonitrile Chromatographically pure: 5:3 Cholesterol esterase Enzyme activity ≥ 10U/mg: 5:4 Methyl tert-butyl ether Chromatographically pure:
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