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GB/T 38421-2019 English PDF

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GB/T 38421-2019: Fur - Detection of animal derived material - Qualitative real-time polymerase chain reaction (PCR) methods
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 38421-2019199 Add to Cart 3 days Fur - Detection of animal derived material - Qualitative real-time polymerase chain reaction (PCR) methods Valid

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Basic data

Standard ID: GB/T 38421-2019 (GB/T38421-2019)
Description (Translated English): Fur - Detection of animal derived material - Qualitative real-time polymerase chain reaction (PCR) methods
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: Y46
Classification of International Standard: 59.140.30
Word Count Estimation: 10,180
Date of Issue: 2019-12-31
Date of Implementation: 2020-07-01
Quoted Standard: GB/T 6682; GB/T 19495.2; GB/T 21102-2007; GB/T 25165-2010
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration
Summary: This standard specifies the real-time fluorescent PCR detection method for qualitative analysis of animal-derived components in natural fur. This standard applies to the qualitative detection of the origin components of eight kinds of natural fur (mink, rabbit, raccoon, raccoon, horse, cow, goat and sheep).

GB/T 38421-2019: Fur - Detection of animal derived material - Qualitative real-time polymerase chain reaction (PCR) methods


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Fur--Detection of animal derived material--Qualitative real-time polymerase chain reaction (PCR) methods ICS 59.140.30 Y46 National Standards of People's Republic of China Real-time fluorescence characterization of fur-derived components Polymerase chain reaction (PCR) detection method polymerasechainreaction (PCR) methods 2019-12-31 release 2020-07-01 implementation State Administration of Market Supervision Published by the National Standardization Administration

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed by the China Light Industry Federation. This standard is under the jurisdiction of the National Leather Industry Standardization Technical Committee (SAC/TC252). This standard was drafted. Guangzhou Inspection, Testing and Certification Group Co., Ltd., National Leather Quality Supervision and Inspection Center (Zhejiang), Shenzhen Huada Gene Technology Service Co., Ltd., Zhejiang Fangyuan Detection Group Co., Ltd., China Leather Shoes Research Institute Co., Ltd., Shaanxi University of Science and Technology Science, China Jiliang University, Dongguan Weiside Technology Development Co., Ltd. The main drafters of this standard. Qin Fangfang, Huang Xinxia, Sun Hailu, Chen Zongliang, Wang Xuechuan, Feng Aiming, Zhang Chi, Sun Xia, Zhao Yang, and Zhang Wenfu. Real-time fluorescence characterization of fur-derived components Polymerase chain reaction (PCR) detection method

1 Scope

This standard specifies a real-time fluorescent PCR detection method for the qualitative analysis of animal-derived components in natural fur. This standard applies to the qualitative detection of the origin of eight natural furs (mink, rabbit, mule, raccoon, horse, cow, goat and sheep).

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 19495.2 Technical requirements for testing laboratory for genetically modified products GB/T 21102-2007 Qualitative detection of rabbit-derived components in animal-derived feeds Real-time fluorescent PCR GB/T 25165-2010 Qualitative detection method for bovine, sheep and pig-derived components in gelatin 3 Terms and definitions, acronyms 3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 Real-time polymerase chain reaction Add a fluorescent group to the polymerase chain reaction system, and use the accumulation of fluorescent signals to monitor the entire PCR process in real time, and Method of qualitative analysis of unknown template by increasing curve. Note. Rewrite GB/T 19495.4-2018, definition 3.1.2. 3.1.2 Ct value cyclethreshold The number of cycles that the fluorescence signal in each reaction tube goes through when it reaches a set threshold. [GB/T 19495.4-2018, definition 3.1.5] 3.2 Acronyms The following abbreviations apply to this document. DNA. deoxyribonucleicacid DTT. dithiothreitol dNTPs. deoxyribonucleoside triphosphate FAM. 6-carboxy-fluorescein Na2EDTA. ethylenediaminetetraaceticaciddisodiumsalt PCR. polymerase chain reaction SDS. sodium dodecylsulfate
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