GB/T 37746-2019 English PDF

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GB/T 37746-2019: Triplex RT-PCR assay for detection of grass carp reoviruses
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 37746-2019	279	Add to Cart	3 days	Triplex RT-PCR assay for detection of grass carp reoviruses	Valid

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Basic data

Standard ID: GB/T 37746-2019 (GB/T37746-2019)
Description (Translated English): Triplex RT-PCR assay for detection of grass carp reoviruses
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 65.020.30
Word Count Estimation: 14,151
Date of Issue: 2019-06-04
Date of Implementation: 2020-01-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 37746-2019: Triplex RT-PCR assay for detection of grass carp reoviruses

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Triplex RT-PCR assay for detection of grass carp reoviruses ICS 65.020.30 B41 National Standards of People's Republic of China Triple RT-PCR detection method for grass carp reovirus Published on.2019-06-04 2020-01-01 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic This standard is under the jurisdiction of the National Aquaculture Standardization Technical Committee (SAC/TC156). This standard was drafted. Zhujiang Fisheries Research Institute, Chinese Academy of Fishery Sciences. The main drafters of this standard. Zeng Weiwei, Wang Qing, Wu Shuqin, Wang Yingying, Shi Cunbin, Song Xinjian, Li Yingying, Zhao Changchen.

Introduction

The issuing body of this document draws attention to the fact that, when the statement conforms to this document, it may involve Chapter 7 "Operating Procedures" and Appendix B. "" and "Triple PCR detection primer set, kit and method for diagnosing grass carp reovirus" (patent number. ZL201210039379.8), etc. Use of related patents. The issuing organization of this document has no position on the authenticity, validity and scope of the patent. The holder of the patent has assured the issuing authority of this document that he is willing to work with any applicant on reasonable and non-discriminatory terms and conditions. Negotiate on patent licensing. The patent holder's statement has been filed with the issuing authority of this document. Related information can be passed the following Contact information obtained. Patent holder. Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences Address. Zhujiang Fisheries Research Institute, No. 1 Xingyu Road, Xitun, Liwan District, Guangzhou Please note that in addition to the above patents, certain aspects of this document may still involve patents. The issuing organization of this document does not undertake to identify these special Liability. Triple RT-PCR detection method for grass carp reovirus

1 Scope

This standard specifies three RT-PCR detection techniques for three different genotypes of grass carp reovirus. This standard is applicable to the detection of nucleic acids of three genotype reoviruses of grass carp and the identification of isolates.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods SC/T 7103 Technical specification for quarantine sampling of aquatic animals

3 Abbreviations

The following abbreviations apply to this document. DEPC. diethylpyrocarbonate (diethypyrocarbonate) dNTP. deoxy-riboncleosidetriphosphate EB. ethidium bromide (ethidiumbromide) GCRV. grass carp reovirus (grasscarpreovirus) M-MLVRT. Moloney murine leukemia virus reverse transcriptase (moloneymurineleukemiavirusreversetran- Scriptase) PBS. phosphate buffer solution PCR. polymerase chain reaction (polymerase chain reaction) RNA. ribonucleic acid RNaseinhibitor. RNase inhibitor RT-PCR. reverse-transcription polymerase chain reaction TaqDNApolymerase. Taq DNA polymerase Triple RT-PCR. triple reverse transcription-polymerase chain reaction (triplexreversetranscriptasepolymerasechainreac- Tion)

4 Technical principles

Grass carp reovirus (GCRV) has three genotypes of type I, II and III, which are shared according to different strains of the same genotype. The sequences are designed with specific primers, which can specifically amplify the nucleic acids of the corresponding genotype isolates, but cannot amplify other genes. A nucleic acid of a type isolate. Three pairs of conserved primers for three genotypes were added to the same polymerase chain reaction (PCR) reaction system. Simultaneous amplification of three nucleic acid fragments of different sizes can be distinguished by the molecular weight of the electrophoresis bands of agarose gel electrophoresis. The purpose of specifically detecting and identifying different genotypes of GCRV is achieved. ......

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