GB/T 37628-2019 English PDF

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GB/T 37628-2019: Determination of astragaloside, paeoniflorin, forsythin and forsythoside A in cosmetics - High performance liquid chromatography
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 37628-2019	279	Add to Cart	3 days	Determination of astragaloside, paeoniflorin, forsythin and forsythoside A in cosmetics - High performance liquid chromatography	Valid

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Basic data

Standard ID: GB/T 37628-2019 (GB/T37628-2019)
Description (Translated English): Determination of astragaloside, paeoniflorin, forsythin and forsythoside A in cosmetics - High performance liquid chromatography
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: Y42
Classification of International Standard: 71.100.70
Word Count Estimation: 14,153
Date of Issue: 2019-06-04
Date of Implementation: 2020-01-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 37628-2019: Determination of astragaloside, paeoniflorin, forsythin and forsythoside A in cosmetics - High performance liquid chromatography

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Determination of astragaloside, paeoniflorin, forsythin and forsythoside A in cosmetics--High performance liquid chromatography ICS 71.100.70 Y42 National Standards of People's Republic of China Astragaloside, paeoniflorin, forsythin and Determination of Forsythiaside A by High Performance Liquid Chromatography Determinationofastragaloside, paeoniflorin, forsythinandforsythosideAin Published on.2019-06-04 2020-01-01 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is under the jurisdiction of the National Spice and Fragrance Cosmetics Standardization Technical Committee (SAC/TC257). This standard was drafted. Hebei Food Inspection Institute (National Fruit and Agricultural Processing Products Quality Supervision and Inspection Center), Suzhou Genealogy Testing Technology Co., Ltd., Shanghai Daily Chemical Industry Research Institute. The main drafters of this standard. Zhang Yan, Li Hui, Cao Meirong, Sun Wenyi, Wu Chunmin, Zhao Xiaoya, Liu Liang, Liu Hongyu, Meng Zhijuan, Wang Juan, Wang Yan, Zhang Chunlin, Dai Dan. Astragaloside, paeoniflorin, forsythin and Determination of Forsythiaside A by High Performance Liquid Chromatography

1 Scope

This standard specifies the qualitative and quantitative HPLC method for the content of astragaloside, paeoniflorin, forsythin and forsythiaside A in cosmetics. The principle, equipment and materials of the detection method, analysis steps, calculation of results, recovery and precision, allowable difference, etc. This standard applies to the determination of the content of astragaloside, paeoniflorin, forsythin and forsythiaside A in water, emulsion and cream. The detection limit of this standard method for astragaloside IV is 15mg/kg, the limit of quantification is 45mg/kg; the detection limit of paeoniflorin is 3mg/kg, The limit is 8 mg/kg; the detection limit of forsythin and forsythiaside A is 20 mg/kg, and the limit of quantification is 60 mg/kg.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 astragaloside IV

3.1 Principle Astragaloside is dissolved in hot water and then extracted with water-saturated n-butanol, washed with ammonia solution, purified by C18 solid phase extraction column, high performance liquid chromatography Separation, evaporative light scattering detector measurement, external standard method for quantification. 3.2 Reagents and materials Unless otherwise specified, the reagents used are chromatographically pure, and the water is the first grade water specified in GB/T 6682. 3.2.1 Methanol. 3.2.2 20% methanol solution. Measure methanol (3.2.1) 20 mL and mix with 80 mL of water. 3.2.3 n-butanol. analytically pure. 3.2.4 Acetonitrile. 3.2.5 Ammonia. 3.2.6 Ammonia test solution. Take 400 mL of ammonia water (3.2.5) and dilute to 1 L with deionized water. 3.2.7 Astragaloside standard substance. purity is not less than 96%. Chinese and English name, CAS number, molecular formula and relative molecular mass of astragaloside See Table A.1 in Appendix A for the structural formula. 3.2.8 Standard working solution of astragaloside. accurately weigh 100mg of astragaloside standard substance (3.2.7) (accurate to 0.1mg) In a 100mL volumetric flask, dilute to the mark with methanol, prepare a standard stock solution of astragaloside IV at a concentration of 1000mg/L, and avoid it at 4°C. Light preservation, valid for 2 months. Accurately transfer 2 mL, 3 mL, 4 mL, 5 mL, 10 mL, 50 mL of the standard stock solution of Astragaloside After 100 mL of the brown volumetric flask, the volume was adjusted to the mark with methanol, and the mass concentrations were 20 mg/L, 30 mg/L, and 40 mg/L, respectively. 50mg/L, 100mg/L, 500mg/L of the standard working solution of astragaloside, stored at 4 ° C in the dark, can be used for one week. 3.2.9 C18 solid phase extraction column..200 mg, 3 mL. ......

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