GB/T 37508-2019 English PDF

US$139.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 37508-2019: Determination of preservatives in moulding clay - High performance liquid chromatography method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 37508-2019	139	Add to Cart	3 days	Determination of preservatives in moulding clay - High performance liquid chromatography method	Valid

Similar standards

GB/T 37647   GB/T 36668.2   GB/T 36668.1   GB/T 45277   GB/T 45275   

Basic data

Standard ID: GB/T 37508-2019 (GB/T37508-2019)
Description (Translated English): Determination of preservatives in moulding clay - High performance liquid chromatography method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: Y57
Classification of International Standard: 97.190
Word Count Estimation: 7,776
Date of Issue: 2019-06-04
Date of Implementation: 2019-10-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 37508-2019: Determination of preservatives in moulding clay - High performance liquid chromatography method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of preservatives in moulding clay--High performance liquid chromatography method ICS 97.190 Y57 National Standards of People's Republic of China Determination of preservatives in modelling clay High performance liquid chromatography 2019-06-04 release 2019-10-01 implementation State Administration of Market Supervision Published by China National Standardization Administration

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed and managed by the National Technical Committee for Standardization of Inspection Methods for Key Products of Quality Supervision (SAC/TC374). This standard was drafted. Taizhou Product Quality Supervision and Inspection Institute, China Inspection and Warner (Beijing) Quality Technology Center Co., Ltd., and China Inspection Alliance (Beijing) Quality Inspection Technology Research Institute Co., Ltd., Xinjiang Uygur Autonomous Region Product Quality Supervision and Inspection Institute, Luohe Luofu Stationery Manufacturing Co., Ltd. Co., Ltd., Pony Testing Group Shanghai Co., Ltd. The main drafters of this standard. Huang Yinbo, Chen Yan, Gao Junwei, Ye Ping, Meng Jie, Wen Haihua, Wang Aixia, Liu Li, Li Hongyi, Zheng Cunzhe, Yan Jinghui, Song Wei. Determination of preservatives in modelling clay High performance liquid chromatography

1 Scope

This standard specifies the method for the determination of preservatives in modeling clay (also known as "color mud") by high-performance liquid chromatography. This standard applies to children's labor, school teaching, model design, art modeling, etc., using flour or polymer resin as the main material 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, phenol, 1,2-benzyl isothiazolin- 3-keto Determination.

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Principle

2-methyl-4-isothiazoline-3-one, 5-chloro-2-methyl-4-isothiazoline-3-one, phenol, 1,2-benzylisothiazoline- 3-keto meridian Ultrasonic extraction, filtration, determination by high performance liquid chromatography, qualitative analysis based on retention time, quantification by external standard method

4 Reagents and materials

Unless otherwise specified, only analytical grade reagents or above are used. 4.1 Water, GB/T 6682, secondary. 4.2 Methanol. chromatographically pure. 4.3 Reference materials. 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, phenol, 1,2-benzylisothiazoline -3- Ketone, purity is not less than 98.0%. See Appendix A for the English name, CAS registration number and molecular formula of the reference material. 4.4 Standard stock solution. Weigh 10 mg (accurate to 0.1 mg) of 2-methyl-4-isothiazolin-3-one (4.3) and 5-chloro-2-methyl-4-isothia The oxazolin-3-one (4.3), phenol (4.3), and 1,2-benzylisothiazolin-3-one (4.3) were placed in 4 different 10 mL volumetric flasks, and The alcohol (4.2) is dissolved, the volume is adjusted to the mark, and shaken to obtain 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, Phenol, 1, A standard stock solution of 2-benzylisothiazolin-3-one at a concentration of 1000 mg/L. Stored at 0 ℃ ~ 4 ℃, the validity period of standard stock solution is 3 months. 4.5 Mixed standard intermediate solution. 0.5mL of 2-methyl-4-isothiazoline-3-one and 1mL of 5-chloro-2-methyl-4-isothiazoline-3- Ketone, 2 mL of phenol, and 2 mL of 1,2-benzyl isothiazolin-3-one standard stock solution (4.4) in the same 10 mL volumetric flask with methanol (4.2) Make up to volume and shake to obtain 2-methyl-4-isothiazolin-3-one concentration of 50mg/L, 5-chloro-2-methyl-4-isothiazolin-3-one concentration A mixed standard intermediate solution of 100 mg/L, a phenol concentration of.200 mg/L, and a 1,2-benzylisothiazolin-3-one concentration of.200 mg/L. 4.6 Mixed standard working solution. Pipette mixed standard intermediate solution (4.5) 0.1mL, 0.2mL, 0.4mL, 1mL, 2mL in 5 10mL In a volumetric flask, make up to volume with methanol (4.2) and shake to obtain 5 mixed standard working solutions of different concentrations. Mixed standard working solution The concentration of each component is shown in Table 1. Table 1 Concentration of each component in the mixed standard working solution name concentration mg/L 2-methyl-4-isothiazolin-3-one 0.5 1.0 2.0 5.0 10.0 5-chloro-2-methyl-4-isothiazolin-3-one 1.0 2.0 4.0 10.0 20.0 Phenol 2.0 4.0 8.0 20.0 40.0 1,2-benzylisothiazolin-3-one 2.0 4.0 8.0 20.0 40.0 4.7 Filter. Organic filter with a pore size of 0.45 μm.

5 Instruments and equipment

5.1 High Performance Liquid Chromatograph. equipped with UV variable wavelength detector. 5.2 Electronic balance. Sensitivity 0.1mg. 5.3 Ultrasonic cleaner. 5.4 Vortex mixer.

6 Analysis steps

6.1 Sample processing Cut the sample into small pieces less than 3mm × 3mm × 3mm, and weigh 0.5g (accurate to 0.1mg) small samples in 10mL In a centrifuge tube with a stopper, add 10 mL of methanol (4.2), seal, vortex on a vortex mixer (5.4) for 1 min, sonicate for 60 min, and cool It was measured at room temperature after filtration through a filter (4.7). 6.2 Instrument settings The measurement conditions of the high performance liquid chromatography (5.1) are set as follows. a) Chromatographic column. C18 column (250mm × 4.6mm, 5μm), or equivalent column; b) Mobile phase. methanol water = 20 80 (volume ratio); c) Flow rate. 1.0mL/min; d) Detection wavelength. 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, phenol, 275nm; different Thiazolin-3-one, 320nm; e) Column temperature. 35 ℃; f) injection volume. 10 μL; g) running time. 30min; h) Post-running time. 10min. Note. The chromatographic conditions such as flow ratio and flow rate vary with the instrument.The operator can choose the best operating conditions according to the test to make the target peak and interference peaks completely Separation. 6.3 Determination Under the chromatographic conditions set in 6.2, take the mixed standard working solution (4.6) and inject them separately. Record the chromatogram. The area is the ordinate and a standard working curve is drawn. Take a sample of the test solution obtained in 6.1 and record the chromatogram to characterize the retention time. Measure the peak area and obtain it according to the standard working curve. The mass concentration of each component in the test solution. The response value of the test object in the sample solution should be within the linear range of the standard curve and exceed the line. The range should be determined after dilution. 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, phenol, and 1,2-benzylisothiazolin-3-one Mixed standard See Appendix B for the liquid chromatogram of the working solution. 6.4 Blank test Except that the sample is not weighed, the same test procedure (6.1 ~ 6.3) as the sample test is adopted.

7 Results calculation

The result is calculated according to formula (1). wi = (ci-c0) × m × f (1) Where. wi --- the content of the measured component in the sample, the unit is milligram per kilogram (mg/kg); ci --- Obtain the mass concentration of the component to be measured in the sample solution from the standard working curve, the unit is milligram per liter (mg/L); c0 --- Obtain the mass concentration of the component to be measured in the blank solution from the standard working curve, the unit is milligram per liter (mg/L); V --- sample solution volume, unit is milliliter (mL); f --- dilution factor; m --- sample mass in grams (g). The calculation results retain two significant digits.

8 Limit of detection and limit of quantitation

The detection limit of 2-methyl-4-isothiazolin-3-one and 5-chloro-2-methyl-4-isothiazolin-3-one is 0.9 mg/kg, and the limit of quantification is 3.0 mg/kg; The detection limit of phenol and 1,2-benzylisothiazolin-3-one was 1.8 mg/kg, and the limit of quantification was 6.0 mg/kg.

9 Precision

The absolute difference between two independent determination results obtained under repeatable conditions should not exceed 10% of the arithmetic mean.

Appendix A

(Normative appendix) Chinese name, English name, CAS number and molecular formula of the standard substance The Chinese name, English name, CAS number and molecular formula of the standard substance are shown in Table A.1. Table A.1 Chinese name, English name, CAS number and molecular formula of the reference material Serial number Chinese name English name CAS number Molecular formula 1 2-methyl-4-isothiazolin-3-one 2-Methyl-4-isothiazolin-3-one 2682-20-4 C4H5NOS 2 5-chloro-2-methyl-4-isothiazolin-3-one 5-Chloro-2-methyl-4-isothiazolin-3-one 26172-55-4 C4H5Cl2NOS 3 Phenol 108-95-2 C6H5OH 4 1,2-Benzylisothiazolin-3-one 1,2-Benzisothiazolin-3-one 2634-33-5 C7H5NOS

Appendix B

(Informative appendix) Liquid chromatogram of mixed standard working solution A typical chromatogram of a mixed standard working solution is shown in Figure B.1. Explanation. 1--2-methyl-4-isothiazolin-3-one (3.529min); 2 --- 5-chloro-2-methyl-4-isothiazolin-3-one (11.367min); 3--phenol (18.423min); 4 --- 1,2-benzylisothiazolin-3-one (25.100min). Figure B.1 Liquid chromatogram of a mixed standard working solution ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 37508-2019_English be delivered?

Answer: Upon your order, we will start to translate GB/T 37508-2019_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 37508-2019_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 37508-2019_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.