GB/T 36851-2018 English PDFUS$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 36851-2018: Detection and identification of Xanthomonas vesicatoria Status: Valid
Basic dataStandard ID: GB/T 36851-2018 (GB/T36851-2018)Description (Translated English): Detection and identification of Xanthomonas vesicatoria Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020.01 Word Count Estimation: 14,127 Date of Issue: 2018-09-17 Date of Implementation: 2019-04-01 Regulation (derived from): National Standard Announcement No. 11 of 2018 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 36851-2018: Detection and identification of Xanthomonas vesicatoria---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection and identification of Xanthomonas vesicatoria ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of pepper bacterial spot disease Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. Publication of this document The organization is not responsible for identifying these patents. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. China Academy of Inspection and Quarantine, Suifenhe Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Chinese People The Gansu Entry-Exit Inspection and Quarantine Bureau of the Republic, the Institute of Biotechnology of the Chinese Academy of Agricultural Sciences, and the Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Tian Hao, Zhang Jian, You Bo, Wang Xiqiao, Zhao Wenjun, Li Weimin, Li Yan. Method for quarantine and identification of pepper bacterial spot disease1 ScopeThis standard specifies the methods for sample preparation, isolation culture and molecular biology of capsicum bacteria. This standard is applicable to the quarantine and identification of samples of pepper plants and seeds that may carry the bacterial spot disease of capsicum.2 Basic information of pepper bacterial spot diseaseChinese name. Capsicum bacterial spot disease Scientific name. Xanthomonasvesicatoria (egDoidge1920) Vauterinetal. (1995) Synonym. Xanthomonascampestrispv.vesicatoria (Doidge)Dye, Bacteriumexitiosum Gardner Kendrick1921, BacteriumvesicatoriaDoidge1920, Bacteriumvesicatorium Doidge1920, Phytomonas Exitiosa (Gardner Monasvesicatoria (Doidge) Bergeyetal.1930, Pkytomonasexitiosum (G. Pseudomonasexitiosa Gardner PseudomonasgardneriSutic1957, Pseudomonasgardnerivar.capsiciSutic1957, Pseudomonasvesi- Catoria (Doidge) Stevens1925, Xanthomonasaxonopodispv.vesicatoria, Xanthomonasaxonopodispv. Vesicatoria (Doidge) Vauterinetal.,.1995, Xanthomonasvesicatoria (D.) Dowson English name. Bacterialleafspotofpepper Classification status. Bacteria, Proteobacteria, γ-Proteobacteria, Yellow Xanthomonodales, Xanthomonodaceae, Xanthomonas. Route of transmission. Capsicum bacterial spot disease is mainly transmitted by long-distance transmission with infected seeds, and also with infected plant debris and stems. And so on. In the greenhouse, the carrier seeds are the only important source of initial infection, but seedling transplanting is also an important route of transmission. Close communication Spread by rain splash or spray irrigation. See Appendix A for additional information on pepper bacterial spotted bacteria.3 Principle of the methodPreliminary determination based on the symptoms of capsicum bacterial spot disease on host plants; according to the specificity of pepper bacterial spot disease DNA sequence for molecular biological detection; culture traits, biological characteristics, host range and hazard based on bacterial bacterial spot disease Isolation, culture and pathogenicity determination of pathogenic bacteria.4 instruments and main reagents4.1 Instruments and equipment PCR instrument, real-time fluorescence PCR instrument, ultra-clean workbench, autoclave, constant temperature incubator, ice machine, high-speed refrigerated centrifuge, table Small centrifuges, ultra-low temperature refrigerators, conventional refrigerators, vortex oscillators, micro-injectors, electrophoresis systems, gel imaging systems, etc. 4.2 Primary reagents All reagents were of analytical grade unless otherwise stated. See Appendix B for the preparation of selective media; reagents for molecular biology testing ...... |
