GB/T 36850-2018 English PDFUS$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 36850-2018: Detection and identification of Tomato severe leaf curl virus Status: Valid
Basic dataStandard ID: GB/T 36850-2018 (GB/T36850-2018)Description (Translated English): Detection and identification of Tomato severe leaf curl virus Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020.01 Word Count Estimation: 14,152 Date of Issue: 2018-09-17 Date of Implementation: 2019-04-01 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 36850-2018: Detection and identification of Tomato severe leaf curl virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection and identification of Tomato severe leaf ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of severe leaf curl virus in tomato Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. China Academy of Inspection and Quarantine, Xiamen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people Heguo Guangdong Entry-Exit Inspection and Quarantine Bureau and Fujian Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Zhang Yongjiang, Liao Furong, Xiang Jun, Feng Lixia, Shen Jianguo. Method for quarantine and identification of severe leaf curl virus in tomato1 ScopeThis standard specifies the quarantine and identification methods for immunological and molecular biology of tomato severe leaf curl virus. This standard applies to the quarantine and identification of host plants and their products that may carry tomato severe leaf curl virus.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products SN/T 2964 Plant Virus Testing Practice3 Basic informationChinese name. Tomato severe leaf curl virus Scientific name. Tomatoesevereleafcurlvirus Abbreviation. ToSLCV Synonym. tomato severe leaf curl virus Taxonomic status. Geminiviridae, a genus of Begomovirus. See Appendix A for additional information on tomato severe leaf curl virus.4 Principle of the methodThe immunogenicity and genomic characteristics of tomato severe leaf curl virus (ToSLCV) are the basis for the quarantine and identification of the virus. According to ToSLCV Specific antibody-to-antibody reaction, the sample was subjected to double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA); according to ToSLCV Genomic features establish polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) detection methods; A combination of effects to determine if the sample has a ToSLCV. 5 instruments, utensils and reagents 5.1 Instrument Electronic balance (sensitivity 0.001g), high-speed centrifuge, small instantaneous centrifuge, ordinary refrigerator, ultra-low temperature refrigerator (-80 ° C), ice machine, Vortex oscillator, magnetic stirrer, autoclave, pH meter, ultra clean bench, PCR machine, microwave oven, electrophoresis, electrophoresis tank, gel imaging Analyzer, microplate reader. 5.2 Appliances Enzyme-linked plate, adjustable pipette (2.5μL, 10μL, 20μL, 100μL,.200μL, 1000μL) and adjustable pipette tip, centrifuge tube ...... |
