GB/T 36809-2018 English PDF

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GB/T 36809-2018: Detection and identification of Moniliophthora perniciosa
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36809-2018	279	Add to Cart	3 days	Detection and identification of Moniliophthora perniciosa	Valid

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Basic data

Standard ID: GB/T 36809-2018 (GB/T36809-2018)
Description (Translated English): Detection and identification of Moniliophthora perniciosa
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,114
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36809-2018: Detection and identification of Moniliophthora perniciosa

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Moniliophthora perniciosa ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of cocoa arbuscular bacteria Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. Publication of this document The organization is not responsible for identifying these patents. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Hainan Entry-Exit Inspection of the People's Republic of China Epidemic situation. The main drafters of this standard. Feng Lixia, Wang Weifang, He Rirong, Wei Shuang, Liu Fuxiu, He Ruifang. Method for quarantine and identification of cocoa arbuscular bacteria

1 Scope

This standard specifies the quarantine and identification methods for the Phytosanitary Phytophthora inoculation (Moniliophthoraperniciosa). This standard applies to the quarantine and identification of cocoa arbuscular bacteria.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 1538.1 Guidelines for the preparation of medium - Part 1

3 Principle of the method

The standard of the disease, the morphological characteristics, the culture characteristics and the molecular biological characteristics of the cocoa arbuscular bacteria on the host is the development of this standard. The main basis. See Appendix A for background information on the taxonomic status, host range, distribution, and symptoms of the disease.

4 equipment

4.1 Instrumentation Stereo microscope, biological microscope, electronic balance (sensitivity 0.001g), autoclave, ultra-clean workbench, biological incubator, PCR Instruments, electrophoresis and gel imaging systems. 4.2 Laboratory equipment Labels, qualitative filter paper, sample bags, storage bags, hand-held magnifiers, tweezers, scalpels, alcohol lamps, inoculation needles, petri dishes (9.0 cm in diameter), Beakers, measuring cylinders, flasks, slides, coverslips, adjustable pipettes (adjustable range 2.5μL~1000μL), centrifuge tubes.

5 reagents and media

5.1 Reagents Penicillin, streptomycin, absolute ethanol, potato, glucose, agar, 70% ethanol, sterile double distilled water, 2% CTAB extraction buffer, 10% SDS solution, 3mol/L sodium acetate solution, PCR reagent (including Taq polymerase, dNTP, buffer), phenol, chloroform, iso Pentanol, Tris-HCl, EDTA (ethylenediaminetetraacetic acid), agarose, molecular weight standard, TAE running buffer. 5.2 medium Potato dextrose agar medium (PDA)..200 g of peeled potato, 20 g of glucose, 18 g of agar, add water to 1000 mL, Autoclave at 121 ° C for 20 min. PDA selective medium. PDA after autoclaving is cooled to 45 ° C ~ 50 ° C, 1000 mL medium under aseptic processing conditions ......

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