GB/T 35942-2018 English PDF

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GB/T 35942-2018: Nested PCR method for detection of Cryptosporidium spp.
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 35942-2018	199	Add to Cart	3 days	Nested PCR method for detection of Cryptosporidium spp.	Valid

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Basic data

Standard ID: GB/T 35942-2018 (GB/T35942-2018)
Description (Translated English): Nested PCR method for detection of Cryptosporidium spp.
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 10,189
Date of Issue: 2018-02-06
Date of Implementation: 2018-09-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 35942-2018: Nested PCR method for detection of Cryptosporidium spp.

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Nested PCR method for detection of Cryptosporidium spp. ICS 11.220 B41 National Standards of People's Republic of China Cryptosporidium nested PCR detection method 2018-02-06 released 2018-09-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized. This standard was drafted. Shanghai Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences, China Animal Health and Epidemiology Center. The main drafters of this standard. Chen Zhaoguo, Mi Rongsheng, Huang Yan, Liu Lu Shi, Wang Yan. Cryptosporidium nested PCR detection method

1 Scope

This standard specifies Cryptosporidium nested PCR detection method. This standard applies to animal detection of Cryptosporidium infection, epidemiological investigation and exit inspection and quarantine.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods Application of Polymerase Chain Reaction (PCR) in Clinical Diagnosis

3 Abbreviations

The following abbreviations apply to this document. PCR. Polymerase Chain Reaction. PBS. Phosphate Buffered Saline. TAE. Tris-acetate buffer (Tris Acetate-EDTA Buffer). EB. EthidiumBromide. BSA. Bovine Serum Albumin. 4 instruments, materials and reagents 4.1 Instrument 4.1.1 PCR gene amplification instrument. 4.1.2 high-speed desktop refrigerated centrifuge. 4.1.3 low-speed high-capacity centrifuge. 4.1.4 trace adjustable pipette. 2μL, 10μL, 20μL,.200μL, 1000μL. 4.1.5 nucleic acid electrophoresis instrument. 4.1.6 nucleic acid electrophoresis tank. 4.1.7 gel imaging analysis system. 4.1.8 biological sample homogeneous mill. 4.1.9 high-pressure steam sterilizer. 4.2 Materials 4.2.1 disposable sealing pouch. 4.2.2 cotton swab. 4.2.3 disposable PE gloves. ......

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