GB/T 35867-2018 English PDF

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GB/T 35867-2018: Inspection of grain and oils -- Determination of phospholipids content in lecithin -- High performance liquid chromatography using a light-scattering detector
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 35867-2018	264	Add to Cart	3 days	Inspection of grain and oils -- Determination of phospholipids content in lecithin -- High performance liquid chromatography using a light-scattering detector	Valid

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Basic data

Standard ID: GB/T 35867-2018 (GB/T35867-2018)
Description (Translated English): Inspection of grain and oils -- Determination of phospholipids content in lecithin -- High performance liquid chromatography using a light-scattering detector
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.040
Word Count Estimation: 14,134
Date of Issue: 2018-02-06
Date of Implementation: 2018-09-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 35867-2018: Inspection of grain and oils -- Determination of phospholipids content in lecithin -- High performance liquid chromatography using a light-scattering detector

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of grain and oils - Determination of phospholipids content in lecithins - High performance liquid chromatography using a light-scattering detector ICS 67.040 X04 National Standards of People's Republic of China Determination of lecithin phospholipid content of grain and oil testing High performance liquid chromatography with evaporative light scattering detection (ISO 11701..2009, Vegetablefats andoils-Determination of phospholipids contentinlecithinsbyHPLCusingalight-scatteringdetector, IDT) 2018-02-06 released 2018-09-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released Directory Foreword Ⅲ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 principle 1 5 Reagents and materials 1 6 instruments and equipment 2 7 sample stick 2 Preparation of test samples 2 9 Analysis Step 3 10 Results Calculation and Presentation 4 11 method precision 4 12 Test Report 4 Appendix A (informative) HPLC Figure 5 Appendix B (informative) joint laboratory test results 6 Reference 9

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard uses the translation method identical with ISO 11701..2009 "Edible vegetable oil HPLC Determination of Evaporative Light Scattering Detection Phospholipids in the content of phospholipids. " The documents of our country that are consistent with the corresponding international documents that are normative references in this standard are as follows. Preparation of animal and vegetable fat samples (ISO 661..2003, IDT). For ease of use, this standard made the following editorial changes. --- Change the standard name (in order to facilitate the existing standard series). This standard proposed by the State Food Administration. This standard by the National Cereals and Oils Standardization Technical Committee (SAC/TC270) centralized. This standard was drafted. Wuhan Food Cosmetics Inspection Institute, Wuhan Product Quality Supervision, Inspection, Wuhan University of Light Industry. The main drafters of this standard. Li Jun, Jiang Xiaoming, Yang Yong, Lu Yupeng, Duan Jiankun, Hu Xiaojing, Fang Huiwen, He Dongping, Zhang Shihong, Zhou Yujuan, Wang Fangfang, Wang Shu. Determination of lecithin phospholipid content of grain and oil testing High performance liquid chromatography with evaporative light scattering detection

1 Scope

This standard specifies the use of high performance liquid chromatography - evaporative light scattering detection quantitative determination of phospholipid content. This standard applies to the crude oil lecithin, oil-free lecithin and lecithin extracted from plant oils. As lysophosphatidylethanolamine, lysophosphatidylinositol, lysophosphatidic acid separation is not good, this standard does not apply to animal lecithin, Ruminant lecithin and enzymatic lecithin.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. ISO 661 Animal and vegetable fats sample preparation (Animalandvegetablefatsandoils-Preparationoftestsam- ple

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 One-component phospholipid contentofanindividualphospholipid The N-acyl-phosphatidylethanolamine (N-acyl-PE), phosphatidylcholine (PC), phosphatidylethanol Amine (PE), phosphatidylinositol (PI), phosphatidic acid (PA), lysophosphatidylcholine (LPC) mass fraction.

4 principle

Each phospholipid fraction was separated by a high performance liquid chromatograph equipped with a diol column, detected by an evaporative light scattering detector, and compared with a standard substance Quantitative.

5 Reagents and materials

Warning - should strictly follow the rules for the use of toxic substances. Take effective measures to ensure the safety of organizations and individuals. Unless otherwise specified, only analytical reagents are used. 5.1 water. a water. 5.2 n-Hexane. Chromatography pure. Isopropyl alcohol. Chromatography pure. Acetic acid. mass fraction ≥ 99.8%. 5.5 Triethylamine. 5.6 Mixed solvent. mixed solvent consists of 80 mL n-hexane (5.2) and 20 mL isopropanol (5.3) (n-hexane volume fraction ......

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