GB/T 35806-2018 English PDF

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GB/T 35806-2018: Animal influenza detection -- Protocol of duplex real-time RT-PCR for influenza virus subtypes H7 and N9
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 35806-2018	199	Add to Cart	3 days	Animal influenza detection -- Protocol of duplex real-time RT-PCR for influenza virus subtypes H7 and N9	Valid

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Basic data

Standard ID: GB/T 35806-2018 (GB/T35806-2018)
Description (Translated English): Animal influenza detection -- Protocol of duplex real-time RT-PCR for influenza virus subtypes H7 and N9
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 10,174
Date of Issue: 2018-02-06
Date of Implementation: 2018-09-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 35806-2018: Animal influenza detection -- Protocol of duplex real-time RT-PCR for influenza virus subtypes H7 and N9

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Animal influenza detection-Protocol of duplex real-time RT-PCR for influenza virus subtypes H7 and N9 ICS 11.220 B41 National Standards of People's Republic of China Animal Flu Detects H7N9 Influenza Virus Dual fluorescent RT-PCR detection method Published on.2018-02-06 2018-09-01 Implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed by the Ministry of Agriculture. This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This standard was drafted by. Beijing Entry-Exit Inspection and Quarantine Bureau Inspection and Quarantine Technology Center, China Entry-Exit Inspection and Quarantine Association, China Agriculture Harbin Institute of Veterinary Science, Chinese Academy of Inspection and Quarantine. The main drafters of this standard. Pu Jing, Gao Zhiqiang, Wang Lin, Qiao Caixia, Zhang Xiquan, Zhou Qi, Zhang Hexiao, Zhang Wei, Yin Hao, Bai Yaxi, Liu Yanhua, Liu Huan, Wang Xiurong, Bao Hongmei, Han Xueqing, and Wang Huiyan. Animal Flu Detects H7N9 Influenza Virus Dual fluorescent RT-PCR detection method

1 Scope

This standard specifies TaqMan dual-fluorescence RT-PCR procedures for the simultaneous detection of influenza A nucleic acid in H7 and N9 subtype animals. method. This standard applies to the rapid detection and screening of animal influenza virus H7 and N9 subtypes.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Test Methods GB/T 19438.1-2004 Avian influenza virus universal fluorescence RT-PCR detection method GB 19489 General Requirements for Laboratory Biosafety

3 Abbreviations

The following abbreviations apply to this document. Ct (or Cp) value. the number of cycles experienced by the amount of fluorescence signal in each reaction tube reaching the set threshold (ThresholdCycle, or CrossingPoint) DEPC. Diethypyrocarbonate FAM. Carboxyfluorescein HA. Hemagglutinin HEX. Hexachlorofluorescein MGB . Minor GrooveBinder NA. Neuraminidase TAMRA. Carboxytetramethylrhodamine Fluorescence RT-PCR. Real-time RT-PCR

4 Reagents and materials

4.1 Reagents 4.1.1 General. Unless otherwise stated, all reagents used are of analytical grade. All liquid reagents used shall be packed in RNase-free containers. 4.1.2 TRIzol. Store at 2°C to 25°C. 4.1.3 Chloroform. Precooling at 2°C to 8°C. 4.1.4 Isopropyl alcohol. Precooled at -20°C. 4.1.5 DEPC water. See A.1 for recipes. ......

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