GB/T 35543-2017 English PDF

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GB/T 35543-2017: Ribonuclease A
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 35543-2017	199	Add to Cart	3 days	Ribonuclease A	Valid

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Basic data

Standard ID: GB/T 35543-2017 (GB/T35543-2017)
Description (Translated English): Ribonuclease A
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C27
Classification of International Standard: 07.080
Word Count Estimation: 10,173
Date of Issue: 2017-12-29
Date of Implementation: 2018-07-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 35543-2017: Ribonuclease A

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Ribonuclease A. ICS 07.080 C27 National Standards of People's Republic of China A ribonuclease A 2017-12-29 Posted 2018-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released Directory Preface Ⅰ Introduction Ⅱ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1

4 technical requirements

5 test methods 1 6 packaging, transportation and storage 2 7 shelf life 2 Appendix A (Normative) RNase A enzyme activity test 3 Appendix B (Normative) Endonuclease assay 5 Appendix C (Normative) Exonuclease assay 6

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard by the National Tooling Standardization Working Group (SAC/SWG11) centralized. This standard was drafted unit. South Fujian Science and Technology Co., Ltd., Fujian Huacan Pharmaceutical Co., Ltd., Shandong University, Beijing University of Chemical Technology, Hua Chan Nan Sheng (Xiamen) Biotechnology Co., Ltd., Fudan University, Beaver Nano Technology (Suzhou) Co., Ltd., Angel Yeast Co., Ltd., Shanghai Boshi Biological Technology Co., Ltd., China Agricultural University, Fujian Agriculture and Forestry University, Suzhou University, Shanghai 100 赛 Biological Technology Co., Ltd. The main drafters of this standard. Huangfa Can, Zheng Dengzhong, Xu Ninglong, Zhan Xuexiong, Chen Xiulan, Zeng Ling Kun, Chen Jinchun, Zhao Jing, Zhang Lili, Huang Fa-xi, Wang Guang-huang, Zhong Jiang, Yao Juan, Liu Bin, Ren Hui, Zhang Xiying, Xing Zhigang, Zhu Li, Li Quanhong, Teresa Teng.

Introduction

Ribonuclease A is derived from bovine pancreas and is an endonuclease that catalyzes RNA degradation, alters the host cell metabolism, inhibits disease Toxicity, but not DNA, can be used to remove contaminated RNA from DNA products. Developed ribonuclease A national standards, To promote the industrialization of this kind of tool enzyme, it is of great significance for the production and use of RNase A. A ribonuclease A

1 Scope

This standard specifies the technical requirements of ribonuclease A, test methods, packaging, transportation, storage and shelf life. This standard applies to the RNA extracted from the bovine pancreas RNA.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 191 Packaging - Pictorial signs

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 An endonuclease capable of cleaving the 3 ', 5'-phosphodiester bond at the pyrimidine base (U and C) of an RNA molecule to form a 2', 3'-cyclic Phosphate derivatives Oligonucleotides. 3.2 The excess of yeast tRNA as a substrate at 37 ° C, pH5.0 conditions, the reaction solution per milliliter per minute at 260nm absorbance value The amount of enzyme required to increase 1.0 is 1 viable unit, ie 1 Pananunit. Note .50Facanunit≈1kunitzunit.

4 technical requirements

4.1 appearance White or almost white powder. 4.2 enzyme activity (specific activity) ≥ 3500Facanunit/mg protein. 4.3 Impurities It should not contain other endonucleases and exonucleases.

5 test methods

5.1 appearance Pour the sample directly or into a colorless, transparent test tube and visually observe under natural light conditions. ......

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