GB/T 35542-2017 English PDFUS$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 35542-2017: Taq DNA polymerase Status: Valid
Basic dataStandard ID: GB/T 35542-2017 (GB/T35542-2017)Description (Translated English): Taq DNA polymerase Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: C27 Classification of International Standard: 07.080 Word Count Estimation: 14,172 Date of Issue: 2017-12-29 Date of Implementation: 2018-07-01 Regulation (derived from): National Standards Bulletin 2017 No. 32 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China GB/T 35542-2017: Taq DNA polymerase---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Taq DNA polymerase ICS 07.080 C27 National Standards of People's Republic of China Taq DNA polymerase 2017-12-29 Posted 2018-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released Directory Foreword Ⅲ Introduction IV 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 technical requirements5 test methods 1 6 packaging, transportation and storage 2 7 shelf life 2 Appendix A (Normative) TaqDNA polymerase enzyme activity test 3 Appendix B (Normative) Exonuclease assay 6 Appendix C (Normative) Endonuclease assay 8ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard by the National Tooling Standardization Working Group (SAC/SWG11) centralized. This standard drafting unit. China Agricultural University, Xiamen Zhi Shan Biotechnology Co., Ltd., Fujian Hua Can Pharmaceutical Co., Ltd., Fujian South Health Technology Co., Ltd., Xiamen University, Hua Can Nan Sheng (Xiamen) Biotechnology Co., Ltd., Beijing University of Chemical Technology, Beaver Nano Technology (Suzhou) Limited, Angel Yeast Co., Ltd., Fudan University, Shandong University, Fujian Agriculture and Forestry University, Suzhou University, Shanghai 100 赛 Biotechnology Co., Ltd. Company, Shanghai Boshi Biological Technology Co., Ltd. The main drafters of this standard. Songna Jie, Huangfa Can, Zheng Dengzhong, He Changhua, Zhan Xuexiong, Li Quanhong, Li Qing Court, Zhao Jing, Chen Jinchun, Chen Xiulan, Yao Juan, Zhang Xiying, Liu Bin, Zhong Jiang, Huang Fa Xi, Ren Hui, Xing Zhigang, Zhu Li, Teresa, Liu Pei, Zhang Lili.IntroductionTaq DNA polymerase is a thermostable enzyme derived from recombinant E. coli and contains all of the clones that are cloned from Thermus species Long TaqDNA polymerase gene, molecular weight of 85ku. The temperature of 55 ℃ ~ 110 ℃ catalytic DNA replication, 74 ℃ ~ 75 ℃ The optimum temperature. TaqDNA polymerase to develop national standards to promote the industrialization of such tools, enzymes, TaqDNA polymerase The production and use is of great significance. Taq DNA polymerase1 ScopeThis standard specifies the technical requirements TaqDNA polymerase, test methods, packaging, transportation, storage and shelf life. This standard applies to TaqDNA polymerase extracted from recombinant E. coli.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 191 Packaging - Pictorial signs.3 Terms and definitionsThe following terms and definitions apply to this document. 3.1 Taq DNA polymerase A thermostable enzyme with a molecular mass of 85 ku derived from recombinant E. coli and containing the full length cloned from Thermus species TaqDNA polymerase gene. The temperature of 55 ℃ ~ 110 ℃ catalytic DNA replication, 74 ℃ ~ 75 ℃ for the optimum temperature. 3.2 Taq DNA polymerase activity unit Using a synthetic hairpin-type oligonucleotide sequence as a template/primer, 1.17 nmol of deoxynucleotides were polymerized into The amount of enzyme required in double stranded DNA is 1 viable unit (U).4 technical requirements4.1 product appearance Clear transparent liquid, no sediment. 4.2 enzyme activity ≥5000U/mL. 4.3 Impurities It should not contain exonucleases and endonucleases.5 test methods5.1 appearance Pour the sample directly or into a colorless, transparent test tube and visually observe under natural light conditions. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 35542-2017_English be delivered?Answer: Upon your order, we will start to translate GB/T 35542-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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