GB/T 35521-2017 English PDF

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GB/T 35521-2017: Chemicals -- Embryotoxicity -- Post-implantation embryo culture test
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 35521-2017	279	Add to Cart	3 days	Chemicals -- Embryotoxicity -- Post-implantation embryo culture test	Valid

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Basic data

Standard ID: GB/T 35521-2017 (GB/T35521-2017)
Description (Translated English): Chemicals -- Embryotoxicity -- Post-implantation embryo culture test
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A80
Classification of International Standard: 13.300; 11.100
Word Count Estimation: 14,120
Date of Issue: 2017-12-29
Date of Implementation: 2018-07-01
Regulation (derived from): National Standards Bulletin 2017 No. 32
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 35521-2017: Chemicals -- Embryotoxicity -- Post-implantation embryo culture test

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals - Embryotoxicity - Post-implantation embryo culture test ICS 13.300; 11.100 A80 National Standards of People's Republic of China Chemical embryotoxicity Whole rat embryo culture after implantation 2017-12-29 Posted 2018-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard by the National Standardization Technical Committee of hazardous chemicals management (SAC/TC251) and focal point. This standard was drafted by China Academy of Inspection and Quarantine, Ningbo Exit Inspection and Quarantine. The main drafters of this standard. Li Hai Shan, Cui Yuan, Han Hui, Chen Huiming, Chen Xiaoqing, Cheng Yan, Ai Wenchao, Xie Wenping. Chemical embryotoxicity Whole rat embryo culture after implantation

1 Scope

This standard specifies the implantation of rat whole embryo culture method to detect chemical embryotoxicity terms and definitions, test principle, test side Law, End Description, Quality Control, Data Analysis and Evaluation. This standard applies to the application of whole rat embryo culture method after testing the embryotoxicity of chemicals. This standard can also be used as drugs, consumption Reference methods for the detection of embryotoxicity of products, agricultural chemicals, food additives and contaminants.

2 Terms and definitions

The following terms and definitions apply to this document. 2.1 Half teratogenic concentration IC50 formalformations IC50 Fifty percent of embryos have deformities of test substance concentrations. 2.2 3T3 half-cell growth inhibitory concentration IC50 for inhibition of hegrowthof3T3cels IC50 (3T3) 50% of 3T3 cell growth is inhibited by the concentration of the test article. 2.3 Maximum teratogenic concentration ICmax formalformations ICmax The maximum ratio of deformity of the test substance minimum concentration. 2.4 Overall morphology score no effect concentration ICNOEC fortotal morphologicalscore ICNOECTMS No significant effect on the overall morphology score the highest concentration.

3 test principle

After 48 h culture of rat embryos with 1-5 body segments, their growth and morphological differentiation were similar to those of the same age in vivo The embryo in the period of organ formation, sensitive to foreign chemicals. Rotary flask culture is conducive to the embryo culture medium and the gas exchange above it. Due to the growing need for oxygen for embryonic development, gas exchange is very important for optimal oxygen saturation of the broth. After the test is completed, The morphology of the embryos was evaluated and the embryos of the control group and the experimental group were compared to obtain embryotoxicity results of the test substance.

4 test methods

4.1 Equipment Use equipment and appliances as follows. ......

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