GB/T 35517-2017 English PDFUS$759.00 · In stock
Delivery: <= 6 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 35517-2017: Chemicals -- Fish short term reproduction toxicity assay Status: Valid
Basic dataStandard ID: GB/T 35517-2017 (GB/T35517-2017)Description (Translated English): Chemicals -- Fish short term reproduction toxicity assay Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: A80 Classification of International Standard: 13.300; 13.020.40 Word Count Estimation: 38,392 Date of Issue: 2017-12-29 Date of Implementation: 2018-07-01 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China GB/T 35517-2017: Chemicals -- Fish short term reproduction toxicity assay---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Chemicals-Fish short term archetype toxicity assay ICS 13.300; 13.020.40 A80 National Standards of People's Republic of China Short-term test methods for chemical fish reproductive toxicity Published on.2017-12-29 2018-07-01 Implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Administration released Directory Preface III Introduction IV 1 Scope 1 2 Terms, definitions and abbreviations 1 3 Method Overview 1 4 Quality Assurance and Quality Control 2 5 Equipment 2 6 Test preparation 2 6.1 Test Water 2 6.2 Domestication of fish 2 6.3 Pre-exposure and fish selection 3 7 Test procedure 3 7.1 General instructions 3 7.2 Selection of test concentration 3 8 Test procedure 3 8.1 Selection and weighing of test fish 3 8.2 Exposure conditions 3 8.2.1 Time 3 8.2.2 Domestication 3 8.2.3 Lights and Temperature 4 8.3 Test frequency 4 8.4 Observation 4 8.4.1 Mortality 4 8.4.2 Behavior and appearance 4 8.4.3 Fertility 4 8.4.4 Euthanasia of fish 4 8.4.5 Observing Secondary Characteristics 4 8.4.6 Determination of vitellogenin 5 8.4.7 Gonadal Histopathology Evaluation 5 9 Data and Reports 5 9.1 Evaluation of Biomarker Response Using ANOVA 9.2 Test Result Report 5 Appendix A (informative) Sample collection procedure for vitellogenin analysis 7 Appendix B (informative annex) Assessment of the detection of secondary sexual characteristics of blackheadhead and barley with specific endocrine-active substances16 Appendix C (Informative Appendix) Experimental Conditions for Fish Endocrine Screening Test 21 Appendix D (informative) Spawning substrates for zebrafish and blackheaded fish23 Appendix E (Informative) Some Chemical Characteristics of Acceptable Dilution Water 25 Appendix F (Informative Appendix) Analysis of Yolk Protein Addition and Inter-Platform Standards 26 Appendix G (Informative) Statistical Analysis Process 27 Appendix H (Normative Appendix) Standards for Interpretation and Acceptability of Test Results 28 References 29 Figure A.1 Cut along the pectoral fins with scissors 8 Figure A.2 Using scissors along the abdomen line into the abdomen about 2mm from the head of the anus Figure A.3 Using surgical forceps to open the abdominal wall, exposing the liver and other internal organs (or securing the abdominal wall laterally) 9 Figure A.4 Slowly separating and resecting the liver with forceps 10 Figure A.5 Slowly removing the intestine with surgical forceps 10 Figure A.6 End of the bowel and any mesentery appendages cut with scissors 11 Figure A.7 (female) Same for the female fish process 11 Figure A.8 Complete the entire process 12 Figure A.9 Segmentation of zebrafish fish head 15 Figure B.1 Number and size of black-headed fish beads 17 Figure B.2 Gender Differences in Anal Fin Shape and Size 19 Figure B.3 Protuberance process on the joint plate of the hip fin line 20 Figure B.4 Fish with cutting points Photo 20 Figure D.1 Zebrafish Spawning Substrates 23 Figure D.2 Blackhead spawning substrate 24 Figure G.1 Statistical analysis flow chart 27 Table B.1 Blackhead Fish Star Template 18 Table C.1 Experimental conditions for the fish endocrine screening test 21 Table E.1 Some Chemical Characteristics of Acceptable Dilution Water 25 ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. The technical content of this standard and the Organization for Economic Cooperation and Development (OrganizationforEconomicCooperationandDevelopment, OECD) Test Guideline 229 (2012) "Fish Short Term Reproductive Toxicity Test (2012)" (in English) Consistent technical content. This standard is proposed and managed by the National Standardization Technical Committee for Hazardous Chemicals Management (SAC/TC251). This standard was drafted by. China Academy of Inspection and Quarantine, China National Accreditation Service for Conformity Assessment, Ningbo Entry-Exit Inspection and Quarantine Bureau, Guangdong Province Microbial Analysis and Testing Center, China Chemical Industry Economic and Technological Development Center, and Shanghai Testing Center. The main drafters of this standard. Cheng Yan, Cui Yuan, Tang Wei, Sun Yun, Chen Huiming, Li Haishan, Xie Wenping, Cao Mengran, Yin Haowen, Zhang Jingyi, Zeng Guoji, Mei Chengfang.IntroductionThis test method describes an in vivo screening test that exposes sexually mature male fish to females in the oviposition phase to chemistry 21d in the product. At the end of the 21-day exposure period, the end points of the biomarkers for male and female fish were determined as test chemical substances Secretion activity index. These test endpoints include vitellogenin and secondary characteristics for yolks of black-headed sturgeon, Japanese clams, and zebrafish Protoplasma assays, while secondary sexuality can only be determined on blackheaded and Japanese barley. Monitor fertility every day throughout the testing process; Retention of gonads can assess the reproductive health of the test animals by histopathology and also provide stronger evidence for other test endpoints. Vitellogenin is usually stimulated by circulating endogenous estrogens and is produced by the liver of female oviparous vertebrates. Vitellogenin is The precursor of vitellin, once produced in the liver, enters the ovary from the blood stream and is absorbed and modified by the developing egg. The vitellogenin is incomplete The plasma of the cooked females and males was barely detectable because they lacked enough circulating estrogen but were stung by exogenous estrogens. When stimulated, the liver can synthesize and secrete vitellogenin. The determination of vitellogenin can detect different estrogenic modes of action for chemicals, and the detection of estrogens may pass The production of vitellogenin in males was measured. Reduction of estrogen circulating levels in females, eg by inhibiting the conversion of endogenous males An aromatase that is a natural estrogen 17β-estradiol causes a decrease in vitellogenin levels, which is used to detect aromatase Inhibiting properties of chemicals. Use standardized tests that have been standardized. Use of immunochemical, species-specific enzyme-linked immunosorbent assay (ELISA) assays In the method, the blood of blackheaded fish, the homogenate of the blood or head/tail of the zebrafish, and the liver of the barley were collected as a sample for the measurement of VTG. for Barley, VTG measured in the blood has a good correlation with the liver. Appendix A provides analysis of vitellogenin Sample collection recommended procedure. The vitellogenin can be tested by different methods, but all are based on validated species-specific ELISA. method. The secondary sexual characteristics of males of specific species are observable and quantitatively responsive to endogenous androgen circulating levels. These characteristics are in blackheads. Fish and barley can be reflected, but the zebrafish is not reflected because the zebrafish does not have a quantifiable secondary sexuality. For blackheaded fish, the main indicator of exposure to exogenous substances is the number of bead stars located on the female's lips. For barley, the number of mastoids The major markers of exogenous exposure to androgens in females were composed. Appendix B illustrates the use of blackheaded fish and barley The process of evaluating sexual characteristics. The 21-day fish test included quantitative fecundity and gonadal histopathology. Additional end points may be required in the experiment A more complete assessment of the reproductive health of the subject was conducted, or to prevent the vitellogenin and secondary sexual characteristics from responding to chemical exposure. although Some of the endpoints are clear conclusions (eg male induces vitellogenin formation, female induces formation of bead) but not all of the tests Endpoints (eg, fecundity and gonadal histopathology) directly indicate the mechanism of toxicity. In addition, endocrine disruption To speculate. Although not a specific endocrine effect, due to its sensitivity to endocrine-disrupting active substances, fertility is considered Consider the important end. Because when it and other endpoints are not affected, it is more certain that this compound is likely not to have endocrine dryness. Disturb activity. When fertility is affected, it will provide strong evidence for inference. This test provides further interpretation of data results Guidance on the acceptability of test results. Short-term test methods for chemical fish reproductive toxicity1 ScopeThis standard specifies the methodological overview, quality assurance and quality control, instrumentation and equipment for short-term test methods for chemical fish reproductive toxicity. Preparations, test procedures, test procedures, data and reports. This standard applies to chemical fish reproductive toxicity test. 2 Terms, definitions and abbreviations 2.1 Terms and Definitions The following terms and definitions apply to this document. 2.1.1 Loading rate loadingrate The wet weight of a fish in a unit volume of water. 2.1.2 Carrying density stockingdensity The number of fish per unit volume of water. 2.1.3 Vitellogenin vitelogenin;VTG A phospholipid glycoprotein, a precursor of yolk protein, which is commonly found in sexually mature female individuals of all oviparous animals. 2.1.4 Maximum tolerated concentration maximumtoleratedconcentration;MTC The test article causes the highest test concentration of less than 10% mortality. 2.2 Abbreviations The following abbreviations apply to this document. CV. coefficient of variation ELISA. enzyme-linked immunosorbent assay HPG axis. hypothalamic-pituitary-gonadal axis3 Method OverviewFemales and males in the breeding period were co-exposed to the test substance. According to the selected test fish species its relevant biomarker endpoint Indicators are measured. Among them, sexual characteristics can be visually confirmed after dissection. See Appendix C for other biological test indicators. Set 3 test substances As well as a blank control, a solvent control can be used if necessary. For barley and zebrafish, at least 2 test vessels per test concentration (5 male fish and 5 female fish per test container); for blackhead fish, at least 4 test containers per test concentration (per The test vessel included 2 tail males and 4 females). Exposure was conducted for 21 days and fish were sampled on Day 21 exposure. On the 21st day, all animals were euthanized. For the detection of secondary sexual characteristics of black-headed finfish and barley, refer to Appendix B; Blood samples of zebrafish and blackheaded fish, or zebrafish head/tail tissue homogenate samples for detection of vitellogenin, see Appendix A; for blue Alas, collect the liver for the detection of vitellogenin (see Appendix A). After dissection, the whole gland is fixed for pathological evaluation. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 35517-2017_English be delivered?Answer: Upon your order, we will start to translate GB/T 35517-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 4 ~ 6 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 35517-2017_English with my colleagues?Answer: Yes. 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