GB/T 35342-2017 English PDFUS$359.00 ยท In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 35342-2017: Technical regulation for molecular detection of Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle Status: Valid
Basic dataStandard ID: GB/T 35342-2017 (GB/T35342-2017)Description (Translated English): Technical regulation for molecular detection of Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020 Word Count Estimation: 18,170 Date of Issue: 2017-12-29 Date of Implementation: 2018-07-01 Regulation (derived from): National Standards Bulletin 2017 No. 32 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China GB/T 35342-2017: Technical regulation for molecular detection of Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Technical regulation for molecular detection of Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle ICS 65.020 B16 National Standards of People's Republic of China Pine wood nematode molecular detection and identification of technical regulations Technical regulation of molecular dection of Bursaphelenchus xylophilus (SteinerandBuhrer) Nickle 2017-12-29 Posted 2018-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Committee for Phytosantry Standardization Technical Committee (SAC/TC271). This standard was drafted unit. Nanjing Forestry University, China Academy of Inspection and Quarantine. The main drafters of this standard. Ye Jianren, Wu Xiaoqin, Chen Feng Mao, Ge Jianjun, Huang Lin, Sun wave.IntroductionThe issuing authority of this document draws attention to the fact that the declaration of conformity with this document may relate to the information in paragraphs 5.1.2 and 5.3.2 relating to the molecular marker of pine wood nematode Use of the relevant patents for the primer. The issuing authority of this document has no position whatsoever about the truthfulness, validity and scope of this patent. The patentee has assured the issuing authority of this document that he is willing to work with any applicant on reasonable and non-discriminatory terms and conditions, Negotiate a patent license. The patent holder's statement has been filed with the issuing authority of this document. Related information can be passed below Contact to get. The name of the patent holder Address. No. 159 Longpan Road, Nanjing Forestry University (210037) Please note that in addition to the above patents, some of the contents of this document may still involve patents. The issuing agencies of this document are not responsible for identifying these specializations Lee's responsibility. Pine wood nematode molecular detection and identification of technical regulations1 ScopeThis standard specifies the pine wood nematode molecular detection and identification of technical regulations. This standard applies to entry and exit phytosanitary, phytosanitary and forestry pests survey of pine wood nematode and its host plant and Products carrying pine wood nematode molecular testing identification.2 Terms and definitionsThe following terms and definitions apply to this document. 2.1 Pinewood nematode pinewoodnematode [Bursaphelenchusxylophilus (SteineretBuhrer) Nickle] An invertebrate, belonging to Nematoda, Secernentea, Aphelenchida, Aphelenchoidoidea, Aphelenchoididae, Bursaphelenchinae, (Bursaphelenchus) plant parasitic nematodes. 2.2 Pine wilt disease pinewiltdiseasecausedbypinewoodnematode Also known as pine wilt disease, is a pine devastating forest disease caused by pine wood nematode parasitism in the pine body. 2.3 Molecular detection moleculardetection With functional genes, ribosomal ITS and other regions as the target, using molecular biology methods to identify species at the gene level. 2.4 Host plants and their products pinewoodandwoodproducts Bursaphelenchus xylophilus host plants and their logs, sawn timber, and wood materials used to carry, package, bedding, support and reinforce goods such as wooden crates, Wooden crates, wooden pallets, wooden frames, casks, wooden shafts, wedges, mats, sleepers and lining wood. After artificial synthesis or by heating, pressure and other deep processing Packaging wood materials, except plywood, fibreboard, etc. 2.5 Primer primer A short nucleotide sequence. Its function is as a starting point for nucleotide polymerization, in the polymerization reaction, guide the synthesis of a template Complementary DNA sequence. 2.6 TaqMan Probe TaqManprobe An oligonucleotide sequence labeled with a reporter fluorophore and a quencher fluorophore at each end. When the probe is intact, the reporter group is reported The fluorescent signal emitted is absorbed by the quencher group. During PCR amplification, the 5'-3 'exonuclease activity of the Taq enzyme degrades the probe so that the fluorescence of the reporter The groups are separated from the quenching fluorophore so that the fluorescence monitoring system can receive the fluorescent signal, ie for each amplification of a DNA strand, there is one The formation of optical molecules, fluorescent signals and amplified copy number has a one-to-one relationship. 2.7 Cycle threshold, Cyclethresholdvalue The meaning of the cycle threshold (Ct value) is the inflection point of the fluorescence signal from the background into the exponential growth phase during PCR amplification ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 35342-2017_English be delivered?Answer: Upon your order, we will start to translate GB/T 35342-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. 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