GB/T 33412-2016 English PDFUS$199.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 33412-2016: Determination of hydroxycitric acid in biochemical products -- HPLC method Status: Valid
Basic dataStandard ID: GB/T 33412-2016 (GB/T33412-2016)Description (Translated English): Determination of hydroxycitric acid in biochemical products -- HPLC method Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: G66 Classification of International Standard: 71.040.30 Word Count Estimation: 10,113 Date of Issue: 2016-12-30 Date of Implementation: 2017-07-01 Regulation (derived from): National Standard Notice No.27 of 2016 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China GB/T 33412-2016: Determination of hydroxycitric acid in biochemical products -- HPLC method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Determination of hydroxycitric acid in biochemical products - HPLC method ICS 71.040.30 G66 National Standards of People's Republic of China Determination of hydroxy citric acid in biological products High performance liquid chromatography Determination ofhydroxycitricacidinbiochemicalproducts- HPLCmethod 2016-12-30 release 2017-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released ForewordThis standard is drafted in accordance with the rules given in GB/T 1.1-2009. This standard by the National Biochemical Testing Standardization Technical Committee (SAC/TC387) proposed and centralized. The drafting of this standard. Shanghai Institute of Metrology and Testing Technology. The main drafters of this standard. Liu Gang, Li Lanying, Ding Min, Wen Yanli, Xu Qin, Xu Li. Determination of hydroxy citric acid in biological products High performance liquid chromatography1 ScopeThis standard specifies the method for the determination of hydroxy citric acid in health (functional) biological products by high performance liquid chromatography (HPLC). This standard applies to oral liquid, capsules, granules and other biological products in the determination of hydroxy citric acid.2 normative reference documentsThe following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods3 principleThe citrate in the sample was extracted with water. After centrifugation, the supernatant was filtered through a 0.45 μm microfiltration membrane and separated by high performance liquid chromatography. Detection of external detectors, external standard method of quantitative determination of hydroxyl citrate content.4 reagents and solutionsUnless otherwise stated, the reagents used in this method are of analytical grade and water is a primary water specified in GB/T 6682. 4.1 methanol. pure chromatography. 4.2 Phosphoric acid. 4.3 volume fraction 0.1% phosphoric acid aqueous solution. accurate extraction of 1mL analysis of pure phosphoric acid, diluted with water and volume to 1000mL. 4.4 hydroxy citrate standard (CAS. 6205-14-7). purity ≥ 98%. 4.5 hydroxy citric acid standard stock solution. accurately weighed 1g (accurate to 0.001g) after 95 ℃ ± 2 ℃ drying 2h hydroxy citrate standard Quasi-goods, add appropriate amount of water dissolved, transferred to 100mL volumetric flask, water volume, shake. Hydroxyl citrate standard stock solution concentration To 10.000 mg/mL. 4.6 hydroxy citric acid standard solution. before use, 10.000mg/mL hydroxy citrate standard stock solution diluted with water, made of concentrated 100 mg/mL, 0.500 mg/mL, 0.200 mg/mL, 0.100 mg/mL, 0.010 mg/mL, 0.001 mg/mL hydroxyl Citric acid standard solution.5 instruments and equipmentLaboratory conventional equipment and the following. 5.1 High Performance Liquid Chromatography. with UV detector. 5.2 Analysis of balance. 0.01g, 0.001g. 5.3 high-speed centrifuge. 10000g. 5.4 Filtration. 0.45 m, aqueous phase. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 33412-2016_English be delivered?Answer: Upon your order, we will start to translate GB/T 33412-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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