GB/T 33409-2016 English PDF

US$119.00 · In stock
Delivery: <= 2 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 33409-2016: Determination of the activity of beta-galactosidase -- Spectrophotometric method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 33409-2016	119	Add to Cart	2 days	Determination of the activity of beta-galactosidase -- Spectrophotometric method	Valid

Similar standards

GB/T 678   GB/T 15478   HG/T 3489   GB/T 33411   GB/T 33410   

Basic data

Standard ID: GB/T 33409-2016 (GB/T33409-2016)
Description (Translated English): Determination of the activity of beta-galactosidase -- Spectrophotometric method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: G66
Classification of International Standard: 71.040.30
Word Count Estimation: 6,676
Date of Issue: 2016-12-30
Date of Implementation: 2017-07-01
Regulation (derived from): National Standard Notice No.27 of 2016
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 33409-2016: Determination of the activity of beta-galactosidase -- Spectrophotometric method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the activity of beta-galactosidase - Spectrophotometric method ICS 71.040.30 G66 National Standards of People's Republic of China Determination of β - galactosidase activity by spectrophotometry 2016-12-30 release 2017-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. This standard by the National Biochemical Testing Standardization Technical Committee (SAC/TC387) proposed and centralized. The drafting of this standard. Shenzhen Institute of Measurement and Quality Inspection, China Testing Technology Research Institute. The main drafters of this standard. Zhang Shiwei, Zhou Lihua, Lai Xintian, Chen Shuijian, Tang Dong, Hong Xiaoming, Wang Zhenni, Yang Guowu. Determination of β - galactosidase activity by spectrophotometry

1 Scope

This standard specifies Kluyveromyces lactis, Kluyveromyces fragilis, Kluyveromyces marxianus, Saccharomyces fragilis, large intestine Escherichia coli, Aspergillus melalea, Aspergillus niger, Detection of glycosidase activity. This standard is applicable to the determination of β-galactosidase activity in biochemical reagents and industrial enzymes.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 terms and definitions

The following terms and definitions apply to this document. 3.1 β-galactosidase activity unit beta-galactosidaseactivityunit The amount of enzyme that catalyzes the conversion of one micromolar o-nitrobenzene-β-D-galactoside per minute under specified reaction conditions.

4 principle

β-galactosidase can rapidly catalyze o-nitrobenzene-β-D-galactoside to produce o-nitrophenol and galactose by changing the absorbance value The amount of o-nitrophenol was calculated to calculate the enzyme activity.

5 Instruments and equipment

5.1 pH meter. accurate to 0.01pH. 5.2 Analysis of the balance. the amount of 0.0001g. 5.3 UV-Vis spectrophotometer. Wavelength Accuracy ± 1nm, absorbance value accurate to 0.001. 5.4 1cm quartz cuvette. 5.5 constant temperature water bath. temperature control accuracy ± 0.5 ℃.

6 reagent

Unless otherwise specified, the reagents used in this method are of analytical grade and water is the tertiary water specified in GB/T 6882. ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 33409-2016_English be delivered?

Answer: Upon your order, we will start to translate GB/T 33409-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 2 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 33409-2016_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 33409-2016_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.