GB/T 33273-2016 PDF EnglishUS$135.00 · In stock · Download in 9 seconds
GB/T 33273-2016: Textiles - Determination of triclosan residues Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure Status: Valid
Similar standardsGB/T 33273-2016: Textiles - Determination of triclosan residues---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT33273-2016NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA ICS 59.080.01 W 04 Textiles - Determination of triclosan residues Issued on: DECEMBER 13, 2016 Implemented on: JULY 01, 2017 Issued by. General Administration of Quality Supervision, Inspection and Quarantine of the PRC; Standardization Administration of the PRC. Table of ContentsForeword... 3 1 Scope... 4 2 Normative references... 4 3 Principle... 4 4 Reagents and materials... 5 5 Apparatus... 5 6 Sample pretreatment... 6 7 Determination... 7 8 Blank tests... 13 9 Calculation of results... 13 10 Lower detection limit and recovery rate... 14 11 Precision... 14 12 Test report... 14 Appendix A (Informative) Typical chromatograms of triclosan and its derivative ... 154 Reagents and materialsUnless otherwise stated, the reagents used in the analysis are of analytical grade. 4.1 Water. The pretreatment water is the grade 3 water specified in GB/T 6682. The water for analytical instrument is the grade 1 water specified in GB/T 6682. 4.2 Methanol. Chromatographically pure. 4.3 N-hexane. Chromatographically pure. 4.4 Acetic anhydride. Purity≥98.5%. 4.5 Sodium tetraborate solution, 0.1 mol/L. WEIGH 38.1 g of sodium tetraborate; USE water to dissolve and dilute to 1000 mL. 4.6 Triclosan standard. CAS No. 3380-34-5.Purity >99.5%. 4.7 Standard stock solution. Accurately weigh an appropriate amount of triclosan standard (4.6) to the nearest 0.1 mg; USE methanol (4.2) to prepare a triclosan standard stock solution with a concentration of 100 mg/L. 4.8 Standard working solution. Accurately pipette an appropriate volume of triclosan standard stock solution (4.7); USE methanol (4.2) to dilute to the standard solution with required concentration. Note. The standard stock solution is stored in the refrigerator at 0 °C~4 °C; the expiry date is 6 months. The standard working solution is stored in the refrigerator at 0 °C~4 °C; the expiry date is 3 months. 4.9 Organic-phase filter membrane. 0.45 μm filter membrane.5 Apparatus5.1 Analytical instruments. According to the selected method, select the following instruments. 5.1.1 High-performance liquid chromatograph. Equipped with diode array detector or ultraviolet detector. 5.1.2 Liquid chromatograph-tandem mass spectrometer. Equipped with electrospray ionization source. 5.1.3 Gas chromatograph. Equipped with electron capture detector. times. The upper organic phase is filtered through a filter membrane (4.9); and then analyzed by gas chromatography (5.1.3), gas chromatography-mass spectrometry (5.1.4), or gas chromatography-tandem mass spectrometry (5.1.5). If necessary, perform instrument analysis after dilution.7 Determination7.1 High-performance liquid chromatography 7.1.1 Analysis conditions of high-performance liquid chromatography Since the test results depend on the instrument used, it is impossible to give general parameters for liquid chromatography analysis. The set parameters shall ensure that, during chromatographic determination, the measured component and other components can be effectively separated. The parameters given below are proved to be feasible. a) Chromatographic column. C18 reversed-phase chromatographic column; 250 mm (column length)×4.6 mm (inner diameter)×5 μm (particle size); or equivalent; b) Mobile phase. Methanol + water (90+10, volume ratio); c) Flow rate. 1.0 mL/min; d) Column temperature. 40 °C; e) Detection wavelength. 282 nm; f) Injection volume. 20.0 μL. 7.1.2 Standard working curve According to 7.1.1, the standard working solution (4.8) is injected for determination. USE the triclosan concentration as the abscissa and the peak area as the ordinate, to draw the standard working curve. Under the above liquid chromatography analysis conditions, the liquid chromatogram of the triclosan standard solution is shown in Figure A.1 a) in Appendix A. 7.1.3 Qualitative and quantitative analysis According to 7.1.1, the sample solution (6.2.1) is injected for determination. According to the retention time of chromatographic peak, perform qualitative analysis. When necessary, confirm in conjunction with ultraviolet absorption spectrogram. Based on the peak area of the chromatographic peak, use the external standard method for quantification. f) Injection volume. 1.0 μL; g) Injection method. Splitless injection. 7.3.2 Standard working curve After the standard working solution (4.8) is derivatization pretreated according to the method of 6.2.2, according to 7.3.1, it is injected for determination. USE the triclosan concentration as the abscissa and the peak area as the ordinate, to draw the standard working curve. Under the above conditions, the gas chromatogram of the standard solution of triclosan derivative is shown in Figure A.2 a) of Appendix A. 7.3.3 Qualitative and quantitative analysis According to the analysis conditions of 7.3.1, the sample solution (6.2.2) is injected for determination. According to the retention time of chromatographic peak, perform qualitative analysis. When necessary, confirm in conjunction with gas chromatography-mass spectrometry (7.4). Based on the peak area of the chromatographic peak, use the external standard method for quantification. 7.4 Gas chromatography-mass spectrometry 7.4.1 Analysis conditions of gas chromatography-mass spectrometry Since the test results depend on the instrument used, it is impossible to give general parameters for gas chromatography-mass spectrometry analysis. The set parameters shall ensure that, during chromatographic determination, the measured component and other components can be effectively separated. The parameters given below are proved to be feasible. 7.4.1.1 Analysis conditions of gas chromatography. a) Chromatographic column. DB-5MS quartz capillary column; 30 m (column length)×0.25 mm (inner diameter)×0.25 μm (film thickness); or equivalent; b) Chromatographic column temperature. 150 °C 280 °C (3.5 min); c) Inlet temperature. 280 °C; d) Chromatograph-mass spectrometer interface temperature. 280 °C; e) Carrier gas. Helium, purity≥99.999%. Flow rate is 1.0 mL/min; f) Injection volume. 1.0 μL; g) Injection method. Splitless injection. Open the valve after 1.0 min. to draw the standard working curve. Under the above conditions, the gas chromatography-tandem mass spectrometry chromatogram of the standard solution of triclosan derivative is shown in Figure A.2 c) of Appendix A. 7.5.3 Qualitative and quantitative analysis According to 7.5.1, the sample solution (6.2.2) is injected for determination. According to the retention time of chromatographic peak, perform qualitative analysis. Confirm in conjunction with the mass spectrogram. Based on the peak area of the chromatographic peak, use the external standard method for quantification.8 Blank testsExcept that no sample is added, the determination steps of 6.1~7.5 above are followed.9 Calculation of resultsAccording to formula (1), calculate the content of triclosan in textiles. Where. x - The content of triclosan in the sample, in milligrams per kilogram (mg/kg); c - The concentration of triclosan read from the standard working curve of the sample solution, in milligrams per liter (mg/L); c0 - The concentration of triclosan read from the standard working curve in the blank test, in milligrams per liter (mg/L); V - The final constant volume of the sample, in milliliters (mL); F - The dilution factor of the sample; m - The mass of the sample, in grams (g). The calculation result is shown to one decimal place. When below the lower detection limit, the test result is not-detectable. ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of English version of GB/T 33273-2016 be delivered?Answer: The full copy PDF of English version of GB/T 33273-2016 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice.Question 2: Can I share the purchased PDF of GB/T 33273-2016_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 33273-2016_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. www.ChineseStandard.us -- GB/T 33273-2016 -- Click this link and select your country/currency to pay, the exact amount in your currency will be printed on the invoice. Full PDF will also be downloaded/emailed in 9 seconds.How to buy and download a true PDF of English version of GB/T 33273-2016?A step-by-step guide to download PDF of GB/T 33273-2016_EnglishStep 1: Visit website https://www.ChineseStandard.net (Pay in USD), or https://www.ChineseStandard.us (Pay in any currencies such as Euro, KRW, JPY, AUD).Step 2: Search keyword "GB/T 33273-2016". Step 3: Click "Add to Cart". If multiple PDFs are required, repeat steps 2 and 3 to add up to 12 PDFs to cart. Step 4: Select payment option (Via payment agents Stripe or PayPal). Step 5: Customize Tax Invoice -- Fill up your email etc. Step 6: Click "Checkout". Step 7: Make payment by credit card, PayPal, Google Pay etc. After the payment is completed and in 9 seconds, you will receive 2 emails attached with the purchased PDFs and PDF-invoice, respectively. Step 8: Optional -- Go to download PDF. Step 9: Optional -- Click Open/Download PDF to download PDFs and invoice. See screenshots for above steps: Steps 1~3 Steps 4~6 Step 7 Step 8 Step 9 |
