GB/T 33249-2016 English PDF

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GB/T 33249-2016: Nanotechnology -- Quantification of gold nanorods in living cells -- Extinction spectroscopy
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Basic data

Standard ID: GB/T 33249-2016 (GB/T33249-2016)
Description (Translated English): Nanotechnology -- Quantification of gold nanorods in living cells -- Extinction spectroscopy
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: G80
Classification of International Standard: 71.040.50
Word Count Estimation: 14,174
Date of Issue: 2016-12-13
Date of Implementation: 2017-07-01
Regulation (derived from): National Standard Notice No.189 of 2016
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 33249-2016: Nanotechnology -- Quantification of gold nanorods in living cells -- Extinction spectroscopy

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Nanotechnology - Quantification of gold nanorods in living cells - Extinction spectroscopy ICS 71.040.50 G80 National Standards of People's Republic of China Determination of gold nanorods in live cells of nanotechnology Extinction spectroscopy 2016-12-13 released 2017-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released Directory Preface I Introduction II 1 Scope 1 2 normative reference document 1 3 Terms and definitions 1 Basic principles 1 5 equipment 2 6 Preparation 2 7 Measurement method 3 Calculation of intracellular gold nanorods 9 Uncertainty Source 4 10 Measurements 4 Appendix A (informative) Gold Nano Bar Preparation Example 5 Appendix B (informative) Quantitative determination of live gold nanoparticles Appendix C (Informative Annex) Example of Uncertainty Assessment 8 Appendix D (informative) Verification of extinction spectroscopy by inductively coupled plasma mass spectrometry (ICP-MS) 9 Appendix E (informative) Test report on the determination of gold nanorods in live cells by extinction spectroscopy Form 10 Reference 11

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed by the Chinese Academy of Sciences. This standard is under the national standard of nanotechnology standardization technical committee (SAC/TC279). The drafting of this standard. Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, National Center for Nanoscience. The main drafters of this standard. Xu Haiyan, Wu Xiaochun, Zhang Weiqi, Ji Yinglu, Wen Tao.

Introduction

Gold nanorods are rod-like nanostructures made of gold atoms. Due to its unique physical and chemical properties, gold nanorods are used in organisms Medical testing, biomedical imaging, cancer and heat treatment and drug delivery and so on. When gold nanorods are used in the biomedical field, The content of intracellular gold nanorods is a very critical indicator, which can quantitatively reflect the efficiency of its entry into the cell. For the new nano-carrier And the design of contrast agents with reference value. Based on the extinction properties of gold nanorods, the method can be used to determine the content of gold nanorods in living cells by extinction spectroscopy. To obtain the aggregation information of the gold nanorods in the cells. Determination of gold nanorods in live cells of nanotechnology Extinction spectroscopy

1 Scope

This standard specifies the method for the determination of gold nanorods in living cells by UV/Vis/near infrared extinction spectroscopy. This standard is suitable for quantitative measurement of the content of gold nanorods in live cells, quantitative measurement of other rods within the living cells of precious metal nanostructures The content of the structure can also be carried out with reference to this standard.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB/T 24369.1-2009 Characterization of gold nanorods - Part 1. UV/Vis/near infrared absorption spectroscopy

3 terms and definitions

The following terms and definitions apply to this document. 3.1 Extinction spectrum When the concentration of the substance to be measured and the thickness of the extinction cup are constant, the extinction (or any function of the extinction) corresponds to the wavelength (or any function of the wavelength) The curve. Note. modify GB/T 9721-2006, definition 3.2. 3.2 Surface plasmon resonance surfaceplasmonresonance; SPR When the incident electromagnetic waves are irradiated onto the metal surface, the free electrons in the metal are driven by an electric field with respect to the lattice of positive ions The frequency of the coherent oscillation occurs. [GB/T 24369.1-2009, definition 3.2] 3.3 Gold nanorods (AuNRs) The ratio of the scale to the other two dimensions of the nanometer scale rod-shaped particles made of gold elements is greater than one. in The dimensions on the two dimensions of the smaller dimension should be between 1 nm and 100 nm. Basic principles The surface plasmon resonance of Au nanorods has two characteristic extinction bands, according to the UV/Vis/near infrared absorption spectroscopy method (see GB/T 24369.1-2009) to obtain the gold nanorod extinction spectrum, see Figure 1a), in which the lateral plasmon resonance peak (1) at 480nm ~ 600nm, the peak area is positively correlated with the concentration of gold nanorods; the longitudinal surface plasmon resonance peak (2) is located in the near infrared spectral region, The peak position is determined by the axial ratio, shape, and external dielectric environment of the gold nanorod. In vitro viable cells were phosphate buffered saline (PBS) suspension on peak 1 without ......

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