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GB/T 32131-2015 English PDF

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GB/T 32131-2015: Determination of the activity of horse radish peroxidase -- Colorimetric method
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 32131-2015109 Add to Cart 3 days Determination of the activity of horse radish peroxidase -- Colorimetric method Valid

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Basic data

Standard ID: GB/T 32131-2015 (GB/T32131-2015)
Description (Translated English): Determination of the activity of horse radish peroxidase -- Colorimetric method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: G60;G69
Classification of International Standard: 07.080
Word Count Estimation: 5,525
Date of Issue: 2015-10-09
Date of Implementation: 2016-03-01
Quoted Standard: GB/T 6682
Regulation (derived from): National Standard Announcement 2015 No.31
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This Standard specifies the horseradish peroxidase activity detection method. This Standard applies to biological and chemical reagents, industrial products - Determination of horseradish peroxidase activity.

GB/T 32131-2015: Determination of the activity of horse radish peroxidase -- Colorimetric method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Horseradish peroxidase activity colorimetric detection method) ICS 07.080 G60/69 National Standards of People's Republic of China Horseradish peroxidase activity colorimetric detection method Issued on. 2015-10-09 2016-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard biochemical tests by the National Standardization Technical Committee (SAC/TC387) and focal points. This standard was drafted. Shenzhen Academy of Metrology and Quality Inspection, China Testing Technology Research Institute, Sichuan Exit Inspection and Quarantine, beads Sea-Exit Inspection and Quarantine. The main drafters of this standard. Laixin Tian, \u200b\u200bZhang Shiwei Tang Dong, Zhou Hua, Hu Kexin, Hong Xiaoming, Yanqiong Ying, Liu Bin, Fan Xuejun, Ze, Yang Guowu. Horseradish peroxidase activity colorimetric detection method

1 Scope

This standard specifies the horseradish peroxidase activity detection method. This standard applies to biological and chemical reagents, industrial products - Determination of horseradish peroxidase activity.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Activity unit activityunit At 25 ℃, pH7.0, the catalytic conversion of one micromole of hydrogen peroxide per minute of the enzyme. Principle 4 Horseradish peroxidase can rapidly catalyze the oxidation of guaiacol (also known as guaiacol, chemical formula. C7H8O2) generates Brown Colored neighbors methoxy phenol even calculate the enzyme activity by changing the value of the absorbance at 436nm. Reaction is.

5 equipment and appliances

5.1 pH meter. 5.2 Analysis of balance. a sense of the amount of 0.0001g. 5.3 UV - visible spectrophotometer.
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