GB/T 31270.18-2014 PDF EnglishUS$110.00 · In stock · Download in 9 seconds
GB/T 31270.18-2014: Test guidelines on environmental safety assessment for chemical pesticides - Part 18: Amphibian acute toxicity test Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure Status: Valid
Similar standardsGB/T 31270.18-2014: Test guidelines on environmental safety assessment for chemical pesticides - Part 18: Amphibian acute toxicity test---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT31270.18-2014 GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA ICS 65.100 B 13 Test guidelines on environmental safety assessment for chemical pesticides - Part 18. Amphibian acute toxicity test Issued on: OCTOBER 10, 2014 Implemented on: MARCH 11, 2015 Issued by. General Administration of Quality Supervision, Inspection and Quarantine; Standardization Administration Committee. Table of ContentsForeword ... 3 1 Scope ... 5 2 Terms and definitions ... 5 3 Test overview ... 6 4 Test methods ... 6 5 Test report ... 10 Appendix A ... 11 Appendix B ... 12 References ... 13ForewordGB/T 31270 “Test guidelines on environmental safety assessment for chemical pesticides” is divided into 21 parts. - Part 1. Transformation in soils; - Part 2. Hydrolysis; - Part 3. Phototransformation; - Part 4. Adsorption/Desorption in soils; - Part 5. Leaching in soil; - Part 6. Volatility; - Part 7. Bioconcentration test; - Part 8. Degradation in water-sediment systems; - Part 9. Avian acute toxicity test; - Part 10. Honeybee acute toxicity test; - Part 11. Silkworm acute toxicity test; - Part 12. Fish acute toxicity test; - Part 13. Daphnia sp. acute immobilisation test; - Part 14. Alga growth inhibition test; - Part 15. Earthworm acute toxicity test; - Part 16. Soil microorganism toxicity test; - Part 17. Trichogramma acute toxicity test; - Part 18. Amphibian acute toxicity test; - Part 19. Effects on non-target plants; - Part 20. Livestock short-term dietary toxicity test; - Part 21. Macro-crustacean toxicity test. This Part is Part 18 of GB/T 31270. Test guidelines on environmental safety assessment for chemical pesticides - Part 18. Amphibian acute toxicity test1 ScopeThis Part of GB/T 31270 specifies the amphibian acute toxicity test’s material, conditions, operation, quality control, data processing, test report and other basic requirements. This Part is applicable to amphibian acute toxicity test for chemical pesticide registration. Other types of pesticides may refer for use. This Part is not applicable to volatile and insoluble chemical pesticides.2 Terms and definitionsThe following terms and definitions are applicable to this document. 2.1 Median lethal concentration In the acute toxicity test, the concentration of test substance when it causes 50% of test biology dead, represented in LC50. Note. The unit is mg a.i./L. 2.2 Test substance The substance to be tested in the test. 2.3 Chemical pesticide The pesticide that is synthetically made of chemical substances. Some take active substance in natural product as parent body to imitate and structurally transform, which are called biomimetic synthesis pesticides. Synonym. synthetic organic pesticide [NY/T 1667.1-2008, Definition 2.3.1] 2.4 Technical material The final product that is made of active ingredients and impurities obtained in the manufacturing process. It must not contain visible foreign matters or any additives. If necessary, add a small amount of stabilizer. [NY/T 1667.2-2008, Definition 2.5.1] 2.5 Formulation product The product that is made of pesticide (or parent drug) and additive, and has stable state of use. [NY/T 1667.2-2008, Definition 2.4] 2.6 Semi-static test During the test, replace the liquid at a regular interval (e.g. 24h), so as to maintain the concentration of the test liquid not less than 80% of the initial concentration. 2.7 Static test Do not replace the test liquid during the test. 2.8 Flow-through test Continuously refresh the test liquid during the test.3 Test overviewThe test methods for amphibian acute toxicity are static test, semi-static test, and flow-through test. It shall adopt suitable method according to the nature of the test substance. Respectively prepare different concentrations of the test substances. Observe and record tadpole’s symptoms of poisoning and number of deaths every day during 96 h test. And calculate LC50 values and 95% confidence limits of 24 h, 48 h, 72 h and 96 h respectively.4 Test methods4.1 Material and conditions 4.1.1 Test biology Rana Limnocharis or Xenopus laevis tadpole is recommended. Specific age and appropriate water temperature can refer to Appendix A. If use other amphibians as test material, they shall meet corresponding domestication and test conditions of physiological requirements, and explanation shall be given as - Mortality rate during the test shall not exceed 10%, and no abnormal behavior occurs; - During the test, the dissolved oxygen content of test liquid shall not be less than 60% of air saturation value (ASV).5 Test reportTest report shall at least contain the following items. - Information of test substance, including test pesticide’s common name, chemical name, structural formula, CAS number, purity, basic physical and chemical properties, origin, etc.; - Test biology’s name, origin, size and domesticated situation; - Test conditions, including test temperature, illumination, dilution water’s temperature, dissolved oxygen concentration and pH, etc.; - Test liquid concentration, LC50 value of 24 h, 48 h, 72 h, and 96 h, 95% confidence limits, and calculation method; - If tadpoles of the control group die or have abnormal reactions; - Observed toxic effects, e.g., any abnormal behavior or poisoning symptom of test tadpoles; - Classification of amphibian toxicity refers to Appendix B.Appendix B(Informative) Acute toxicity classification of pesticide to tadpole According to median lethal concentration LC50 (96h) of pesticide to tadpole, classify the acute toxicity of pesticide to tadpole into four grades, as shown in Table B.1. Table B.1 Toxicity classification of pesticides to amphibian Toxicity classification LC50 (96h) / (mg a.i./L) Severe toxicity LC50 ≤ 0.1 High toxicity 0.1 < LC50 ≤ 1.0 Medium toxicity 1.0< LC50 ≤ 10 Low toxicity LC50 >10 References [1] NY/T 1667.1-2008 Terminology of pesticide registration management - Part 1. Basic terminology [2] NY/T 1667.2-2008 Terminology of pesticide registration management - Part 2. Product chemistry [3] OECD (2009). Guideline 231. The Amphibian Metamorphosis Assay, OECD Guidelines for the Testing of Chemicals. [4] US EPA (1996). Tadpole / Sediment Subchronic Toxicity Test (OPPTS 850.1800). Ecological effects test guidelines. [5] ASTM (2007). Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macr. Oinvertebrates, and Amphibians. American Society for Testing and Materials, ASTM E729-96. [6] OECD (l992). Guideline 203. Fish, Acute Toxicity Test. OECD Guidelines for the Testing of Chemicals. [7] Cai Daoji. Research on Pesticide Environmental Toxicology. Beijing. China Environmental Science Press, 1999. ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of English version of GB/T 31270.18-2014 be delivered?Answer: The full copy PDF of English version of GB/T 31270.18-2014 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice.Question 2: Can I share the purchased PDF of GB/T 31270.18-2014_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 31270.18-2014_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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