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Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 31270.11-2025: Test guidelines on environmental safety assessment for chemical pesticides - Part 11: Silkworm acute toxicity test Status: Valid GB/T 31270.11: Historical versions
Basic dataStandard ID: GB/T 31270.11-2025 (GB/T31270.11-2025)Description (Translated English): Test guidelines on environmental safety assessment for chemical pesticides - Part 11: Silkworm acute toxicity test Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B15 Classification of International Standard: 65.100 Word Count Estimation: 14,185 Date of Issue: 2025-08-29 Date of Implementation: 2026-03-01 Older Standard (superseded by this standard): GB/T 31270.11-2014 Issuing agency(ies): State Administration for Market Regulation, National Standardization Administration GB/T 31270.11-2025: Test guidelines on environmental safety assessment for chemical pesticides - Part 11: Silkworm acute toxicity test---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT31270.11-2025 ICS 65.100 CCSB15 National Standard of the People's Republic of China Replaces GB/T 31270.11-2014 Test Guidelines for Environmental Safety Assessment of Chemical Pesticides Part 11.Acute toxicity test for silkworm Released on August 29, 2025 Implementation on March 1, 2026 State Administration for Market Regulation The National Standardization Administration issued Table of ContentsPreface III Introduction V 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Principle 1 5 Test conditions 2 6 Reagents and Materials 2 7 Instruments and Equipment 2 8 Test items 2 9 Test Step 3 10 Experimental data processing 4 11 Quality Control 4 12 Test Report 4 References 5 Preface This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document is Part 11 of GB/T 31270 "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides". GB/T 31270 has been published. The following parts. --- Part 1.Soil metabolism test; --- Part 2.Hydrolysis test; --- Part 3.Photolysis test; --- Part 4.Soil adsorption/desorption test; --- Part 5.Soil leaching test; --- Part 6.Volatility test; --- Part 7.Bioconcentration test; --- Part 8.Water-sediment system metabolism test; --- Part 9.Short-term feeding toxicity test for birds; --- Part 10.Honey bee acute toxicity test; --- Part 11.Silkworm acute toxicity test; --- Part 12.Fish acute toxicity test; --- Part 13.Daphnia acute immobilization test; --- Part 14.Algal growth inhibition test; --- Part 15.Earthworm acute toxicity test; --- Part 16.Soil microbial toxicity test; --- Part 17.Acute toxicity test for natural enemy Trichogramma; --- Part 18.Acute toxicity test for natural enemies and amphibians; --- Part 19.Non-target plant impact test; --- Part 20.Short-term feeding toxicity test for livestock; --- Part 21.Toxicity test for large crustaceans; --- Part 22.Soil surface photolysis test; --- Part 23.Birds acute oral toxicity test. This document replaces GB/T 31270.11-2014 "Test Guidelines for Environmental Safety Assessment of Chemical Pesticides Part 11.Acute Toxicity to Silkworms" Compared with GB/T 31270.11-2014, in addition to structural adjustments and editorial changes, the main technical changes are as follows. a) The definition of the term "50% lethal concentration" has been changed (see 3.1, 2.1 of the.2014 edition); b) Deleted the relevant provisions on test substances, chemical pesticides, technical materials, preparations, active ingredients, and reference substances (see 2.2~ 2.7); c) The test principle has been changed (see Chapter 4, Chapter 3 of the.2014 edition); d) Added reagents required for the test (see 6.1); e) The requirements for test organisms have been changed (see 6.2, 4.1.1 of the.2014 edition); f) Added the requirements for test mulberry leaves (see 6.3); g) The requirements for test substances have been changed (see Chapter 8, 4.1.2 of the.2014 edition); h) The description of the test steps has been changed (see Chapter 9, 4.2 of the.2014 edition); i) The test data processing method has been changed (see Chapter 10, 4.3 of the.2014 edition); j) The reference substance test (see 4.4 of the.2014 edition) has been deleted. Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Technical Committee on Pesticide Standardization (SAC/TC133). This document was drafted by. Pesticide Control Institute, Ministry of Agriculture and Rural Affairs. The main drafters of this document are. Yuan Shankui, Zhang Yan, Zhou Yanming, Chen Lang, Qu Mengmeng, Zhou Xinxin, Liu Liang, Zhao Xiuzhen, Zhu Lizhen, Chen Siqi, Gao Xinyue and Li Jiangyu. The previous versions of this document and the documents it replaces are as follows. ---First published in.2014 as GB/T 31270.11-2014; ---This is the first revision.introductionIn order to reduce the impact of pesticide use on the ecological environment and provide data that meets the requirements of pesticide environmental risk assessment, it is necessary to carry out environmental safety assessment. Tests are conducted to clarify the metabolism, degradation, adsorption, leaching, bioaccumulation and other characteristics of pesticides in the environment as well as their toxicity to non-target organisms. GB/T 31270 "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides" is a test method standard guiding the environmental safety evaluation test of pesticides in my country. The standard aims to standardize the pesticide environmental safety evaluation test work and improve the scientificity and comparability of pesticide environmental safety evaluation tests. It consists of 23 parts. constitute. --- Part 1.Soil metabolism test. The purpose is to clarify the metabolic pathways and metabolic rates of pesticides in soil. --- Part 2.Hydrolysis test. The purpose is to clarify the hydrolysis pathway and hydrolysis rate of pesticides. --- Part 3.Photolysis test. The purpose is to clarify the photolysis pathway and photolysis rate of pesticides. --- Part 4.Soil adsorption/desorption test. The purpose is to clarify the soil adsorption characteristics of pesticides. --- Part 5.Soil leaching test. The purpose is to clarify the leaching of pesticides. --- Part 6.Volatility test. The purpose is to clarify the volatility of pesticides. --- Part 7.Bioconcentration test. The purpose is to determine the bioconcentration of pesticides in fish. --- Part 8.Water-sediment system metabolism test. The purpose is to clarify the metabolic pathways and metabolism of pesticides in water-sediment systems. Thanks for the rate. --- Part 9.Short-term feeding toxicity test for birds. The purpose is to determine the short-term feeding toxicity of pesticides to birds. --- Part 10.Bee acute toxicity test. The purpose is to determine the acute toxicity of pesticides to bees. --- Part 11.Acute toxicity test to silkworm. The purpose is to determine the acute toxicity of pesticides to silkworm. --- Part 12.Fish acute toxicity test. The purpose is to determine the acute toxicity of pesticides to fish. --- Part 13.Daphnia acute activity inhibition test. The purpose is to determine the acute toxicity of pesticides to Daphnia. --- Part 14.Algal growth inhibition test. The purpose is to clarify the toxicity of pesticides to algae. --- Part 15.Earthworm acute toxicity test. The purpose is to determine the acute toxicity of pesticides to earthworms. --- Part 16.Soil microbial toxicity test. The purpose is to clarify the toxicity of pesticides to soil microorganisms. --- Part 17.Acute toxicity test for natural enemy Trichogrammatis. The purpose is to clarify the toxicity of pesticides to Trichogrammatis. --- Part 18.Acute toxicity test for natural enemies and amphibians. The purpose is to clarify the acute toxicity of pesticides to amphibians. --- Part 19.Non-target plant effects test. The purpose is to clarify the toxicity of pesticides to non-target plants. --- Part 20.Short-term feeding toxicity test for livestock. The purpose is to clarify the feeding toxicity of pesticides to livestock. --- Part 21.Toxicity test to large crustaceans. The purpose is to determine the toxicity of pesticides to large crustaceans. --- Part 22.Soil surface photolysis test. The purpose is to clarify the photolysis pathway and photolysis rate of pesticides on the soil surface. --- Part 23.Bird acute oral toxicity test. The purpose is to determine the acute oral toxicity of pesticides to birds. Test Guidelines for Environmental Safety Assessment of Chemical Pesticides Part 11.Acute toxicity test for silkworm Warning. Personnel using this document should have practical experience in regular laboratory work. This document does not address all possible safety issues. The user is responsible for taking appropriate safety and health measures and ensuring that the conditions stipulated by relevant national laws and regulations are met.1 ScopeThis document describes a test method for the acute toxicity of chemical pesticides to silkworms for the environmental safety assessment. This document applies to acute toxicity tests on silkworms conducted for registration of chemical pesticides.2 Normative referencesThe contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories NY/T 2882.5 Guidelines for Environmental Risk Assessment for Pesticide Registration Part 5.Silkworm NY/T 4195.4 Pesticide Registration Environmental Impact Test Biological Material Cultivation Part 4.Silkworm3 Terms and DefinitionsThe following terms and definitions apply to this document. 3.1 In the spray toxicity test, the concentration of the test substance in mulberry leaves that causes 50% of the test organisms to die is expressed as LC50. Note. The unit is mg test substance/kg mulberry leaf or mga.i./kg mulberry leaf. 4 Principles 4.1 Spray toxicity test The second-instar silkworms were fed with sprayed mulberry leaves and the poisoning symptoms and death of the tested silkworms were observed after 24h, 48h, 72h and 96h. After the test, the data were statistically analyzed to determine the half-lethal concentration (LC50) and its 95% confidence interval. 4.2 Fumigation toxicity test Tests on simulating indoor application conditions for sanitary drugs should be conducted in a fumigation test device or fumigation room that meets the test requirements. The fumigation test device or fumigation chamber should be designed with relevant parameters according to the recommended dosage, provided that the test requirements are met. The device is placed in a fumigation chamber for quantitative combustion (or electric heating). From the start of fumigation, observe and record the amount of household items in the fumigation test device at 0.5h, 2h, 4h, 6h, and 8h. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 31270.11-2025_English be delivered?Answer: Upon your order, we will start to translate GB/T 31270.11-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 31270.11-2025_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 31270.11-2025_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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