GB/T 30483-2013 English PDFUS$169.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 30483-2013: Determination of theaflavins in tea -- High performance liquid chromatography Status: Valid
Basic dataStandard ID: GB/T 30483-2013 (GB/T30483-2013)Description (Translated English): Determination of theaflavins in tea -- High performance liquid chromatography Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: X04 Classification of International Standard: 67.140.10 Word Count Estimation: 7,762 Quoted Standard: GB/T 8302; GB/T 8303 Regulation (derived from): National Standards Bulletin 2013 No. 27 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the (HPLC) determination of tea and tea products in the instrumentation, reagents, methods of operation theaflavin content by HPLC, the calculated result. This standard applies to the determination of tea and tea products in the th GB/T 30483-2013: Determination of theaflavins in tea -- High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Determination of theaflavins in tea. High performance liquid chromatography ICS 67.140.10 X04 National Standards of People's Republic of China Tea theaflavins HPLC Issued on. 2013-12-31 2014-06-22 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by China Federation of Supply and Marketing Cooperatives. This standard by the National Standardization Technical Committee of tea (SAC/TC339) centralized. This standard was drafted. China Federation of Supply and Marketing Cooperatives Hangzhou Tea Research Institute, the National Tea Quality Supervision and Inspection Center. The main drafters of this standard. Zhou Weilong, Xu Jianfeng, Lu Xiaolei, Xu Ling, Ye Meijun. Tea theaflavins HPLC1 ScopeThis standard specifies the determination of tea and tea products theaflavin content of the instrument, reagents, methods of operation, the junction by high performance liquid chromatography (HPLC) If the calculations. This standard applies to the determination of tea and tea products theaflavin content also applies to caffeine, catechins and gallic acid determination did not.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 8302 sampling tea Sample Preparation and Determination of dry matter content GB/T 8303 Tea grated Principle 3 Milled sample tea theaflavin (catechins) with 70% methanol solution at 70 ℃ water bath extract, instant tea with hot 10% acetic Nitrile dissolved. Theaflavin (catechins) was measured using C18 column, detection wavelength 278nm, gradient, HPLC analysis, tea flavin (catechins) Standard Substance direct quantitative external standard method.4 Instrument4.1 Analysis of balance. a sense of the amount of 0.0001g. 4.2 water bath. 4.3 centrifuge. speed 3500r/min. 4.4 Mixer. 4.5 high performance liquid chromatography (HPLC). contains gradient elution and UV detector (detection wavelength 278nm). 4.6 Liquid Column. C18 (particle size 5μm, 250mm × 4.6mm).5 Reagents5.1 Unless otherwise indicated, the methods used were of analytical grade reagents (AR), distilled water. 5.2 acetonitrile. chromatography. 5.3 methanol. 5.4 glacial acetic acid. 5.5 70% aqueous methanol (volume fraction). 5.6 10mg/mLEDTA-2Na solution. now with. 5.7 10mg/mL ascorbic acid solution. now with. 5.8 stable solution. respectively 25mLEDTA-2Na solution (5.6), 25mL ascorbic acid solution (5.7), 50mL acetonitrile (5.2) plus ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 30483-2013_English be delivered?Answer: Upon your order, we will start to translate GB/T 30483-2013_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 30483-2013_English with my colleagues?Answer: Yes. 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