GB/T 29764-2013 English PDF

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GB/T 29764-2013: Chemicals -- Fish (Oryzias latipes, d-rR Medaka) early life stage toxicity test
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 29764-2013	369	Add to Cart	3 days	Chemicals -- Fish (Oryzias latipes, d-rR Medaka) early life stage toxicity test	Valid

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Basic data

Standard ID: GB/T 29764-2013 (GB/T29764-2013)
Description (Translated English): Chemicals -- Fish (Oryzias latipes, d-rR Medaka) early life stage toxicity test
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A80
Classification of International Standard: 13.300; 11.100
Word Count Estimation: 16,186
Quoted Standard: GB/T 27861
Regulation (derived from): National Standards Bulletin No. 18 of 2013
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies: medaka fish (d-rR strain medaka, hereinafter referred to as "fish") early life stage toxicity testing of chemicals the method outlined, equipment, test preparation, test procedures, quality assurance and quality control, data and

GB/T 29764-2013: Chemicals -- Fish (Oryzias latipes, d-rR Medaka) early life stage toxicity test

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals. Fish (Oryzias latipes, d-rR Medaka) early life stage toxicity test ICS 13.300; 11.100 A80 National Standards of People's Republic of China Chemicals medaka fish early life stage toxicity test Issued on. 2013-09-18 2014-03-20 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Table of Contents

Introduction Ⅲ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 test substance information 5 Summary of Method 2 6 Equipment 2 7 Test Preparation 2 8 Test Procedure 3 9 Quality Assurance and Quality Control 6 10 Data and Reports 6 Appendix A (informative) medaka (Oryziaslatipes, d-rRMedaka) domestication and breeding and nurturing environment parameters 8 Annex B (informative) qualified diluted chemical characteristics of water 10 Formulating ingredients in Appendix C (informative) hatching fluid of 11 Reference 12

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard reference to the Organisation for Economic Co-operation and Development (OECD) guidelines for testing of chemicals No.210 (1992 Nian) "fish early life stages Toxicity Test "(in English), and adds the following main elements. --- Test biological selection d-rR strain medaka, increased feeding times d-rR strain medaka newly hatched fry, juveniles and parents, investment Feeding Amount and other information; --- Anomalies adds multiple embryos and larvae damage deformity statements; --- An increase in the sex ratio of male and female forms distinguish statements; --- Statistical parameters increased at the end of the test the sex ratio of male and female fish. This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points. This standard was drafted. Research Center for Eco-Environmental Sciences, Chinese Academy of Inspection and Quarantine, the Ministry of Environmental Protection Chemicals Gordon Hutchison Center, Shanghai testing center. The main drafters of this standard. Chagin Miao, Wang Zijian, Li Wei, Zhu Lifei, Lizhao Li, Li Xi, Cheng Yan, Liu Chun new. Chemicals medaka fish early life stage toxicity test

1 Scope

This standard specifies the medaka fish (d-rR strain medaka, hereinafter referred to as "fish") early life stage toxicity test chemical methods overview Equipment, test preparation, test procedures, quality assurance and quality control, data and reports. This standard applies to testing the toxicity of chemicals to fish early life stages (embryonic development and juvenile growth phase).

2 Normative references

The following documents for the application of this document is essential, dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 27861 Fish acute toxicity of chemicals

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Lowest observed effect concentration lowestobservedeffectconcentration; LOEC Compared with the control group, the test substance to produce a significant effect (p < 0.05) the lowest test concentration. 3.2 No observed effect concentration noobservedeffectconcentration; NOEC Directly below the test of the test substance concentration LOEC. 3.3 Median lethal concentration medianlethalconcentration; LC50 Test concentration causing 50% of the test fish died, expressed in LC50.

4 test substance information

a) structural formula; b) purity; c) solubility in water; d) vapor pressure; e) hydrolysis of the dissociation constant (pKa); f) n-octanol/water partition coefficient (Pow); g) stability in water and light in; h) in the test solution and reliable quantitative analysis of the test substance and accuracy, detection limit; i) stability under test conditions; j) d-rR strain medaka LC50 values; k) biodegradability test results. ......

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